Invasion assays were performed using 48-well microchemotaxis chambers that contained 12 µm-pore membranes (Neuroprobe, Gaithersburg, MD, USA) pre-coated with 10 µg/mL Matrigel (BD Bioscience, San Jose, CA, USA) for the invasion assay as described previously [22 (link)]. The cells were seeded in the chambers, in triplicate, together with serum-free media, and incubated for 28 h for invasion. The membranes were fixed and stained using Diff-Quik reagent (Sysmex Corporation, Kobe, Japan). The invaded and migrated cells were photographed under a light microscope, and relative invasion and migration rates were calculated based on comparison with the control.
12 m pore membranes
Manufactured by Neuro Probe
Sourced in United States
The 12 µm-pore membranes are a laboratory equipment product designed for filtration and separation applications. They feature a uniform pore size of 12 micrometers, which allows for the efficient filtration and separation of particles and materials of specific sizes. The membranes are made from durable materials and are suitable for use in a variety of research and industrial settings.
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Lab products found in correlation
2 protocols using 12 m pore membranes
1
Matrigel-coated Invasion Assay
2
Cell Invasion and Migration Assay
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Invasion and migration assays were performed using 48-well micro-chemotaxis Boyden chambers that contained 12-µm-pore membranes (Neuroprobe, Gaithersburg, MD, USA) pre-coated with 10 µg/ml Matrigel (BD Bioscience, San Jose, CA, USA) for the invasion assay and 13 µg/ml collagen type I (Sigma Aldrich) for the migration assay as described previously [14] . The cells, H1299 (0.8 × 10 6 cells/ml) and Hcc1438 (1.3 × 10 6 cells/ml), were seeded in triplicate in the chambers, and incubated for 24-26 h. The resulting membranes were xed and stained using Diff-Quik reagent (Sysmex Corporation, Kobe, Japan). The invaded and migrated cells were photographed under a light microscope, and the relative invasion and migration rates were calculated based on comparison to the negative controls.
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