Ab71753

The protein was separated and transferred, and then incubated with 5% milk for 1 h. The protein was incubated at 4°C overnight using antibodies: PSMC4 (ab139184, Abcam), CBX3 (ab213167, Abcam), tropomodulin 3 (TMOD3, ab157215, Abcam), SUZ12 polycomb repressive complex 2 subunit (SUZ12, ab307891, Abcam), LCK proto‐oncogene (LCK, ab227975, Abcam), beta‐transducin repeat containing E3 ubiquitin protein ligase (BTRC, ab71753, Abcam), PI3 kinase p85 (PI3K, 4292S, CST, USA), phospho‐PI3K (BS‐3332R, Bioss, China), Akt (4685S, CST, USA), phospho‐Akt (4060S, CST, USA), mTOR (2983S, CST, USA), phospho‐mTOR (5536S, CST, USA), EGFR (ab52894, Abcam), Bax (ab32503, Abcam), Bcl‐2 (ab182858, Abcam), Caspase 3 (ab32351, Abcam), Cleaved‐Caspase 3 (C‐Caspase, ab32042, Abcam) and GAPDH (1:3000, AP0063, Bioworld). Then secondary antibody was performed and protein was verified using an imaging system (Tanon 5200, China).

Tumor tissues were homogenized in ice-cold lysis buffer. Protein was extracted from TNBC cells, THP-1 cells, exosomes and tumor homogenates using Protein Extraction Kit (Abcam) and quantified with bicinchoninic acid (BCA) kit (Bio-Rad, Hercules, CA, USA). 30 µg of protein was electrophoresed in 12% SDS-PAGE gel and transferred to polyvinylidene fluoride (PVDF) membranes (Bio-Rad). Membranes were blocked and incubated with rabbit antibodies against CD63 (1:1000, ab193349, clone: MX-49.129.5, Abcam), CD9 (1:1000, ab236630, clone: EPR23105-121, Abcam), TSG101 (1:2000, ab125011, clone: EPR7130(B), Abcam), HSP70 (1:500, ab2787, clone: 5A5, Abcam), Calnexin (1:1000, ab22595, Abcam), GM130 (1:1000, ab52649, clone: EP892Y, Abcam), E-cadherin (1:500, ab231303, clone: 4A2, Abcam), N-cadherin (1:1000, ab76011, clone: EPR1791-4, Abcam), Vimentin (1:500, ab92547, clone: EPR3776, Abcam), Snail (1:500, PA5-23482, Thermo Fisher Scientific), TFEB (1:1000, ab264421, clone: BLR070G, Abcam), BTRC (1:800, ab71753, Abcam) and GAPDH (1:5000, ab8245, clone: 6C5, Abcam) overnight. Membranes were rinsed and incubated with an HRP-conjugated secondary antibody. Enhanced chemiluminescence (ECL) substrate (Beyotime) was used to visualize bands. Antibodies were ordered from Abcam. Uncropped scans were supplied in Supplementary Figure no. 3.