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2 protocols using p xylene d10

1

Determination of Volatile Amines in Seafood

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All solvents and analytical standards of dimethylamine (DMA), trimethylamine (TMA), trimethylamine-N-oxide (TMAO), cadaverine (CAD), hydrogen peroxide solution, anisole, guaiacol, and p-xylene-d10 were purchased from Sigma Merck (Merck, Milan, MI, Italy). High-performance liquid chromatography (HPLC)-grade water was obtained from a MilliQ Academic water purification system (Millipore, Milan, Italy). Before use, solvents were filtered through a 0.45 µm filter and degassed for 10 min in an ultrasonic bath.
Fishery products samples were: Atlantic bonito (Sarda sarda) mackerel-like fish of the family Scombridae; European squid (Loligo vulgaris) belonging to the family Loliginidae. Atlantic bonito and European squid samples were purchased in a local market or caught fresh in Tyrrhenian Sea and brought in the laboratory no later than 5 h after fishing, in ice.
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2

Headspace GC-MS Analysis of Terpenes

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The reference compounds (-)-menthol, menthone, menthyl acetate, and p-xylene-d10 were of analytical grade and purchased from Sigma-Aldrich (Zwijndrecht, the Netherlands). Methanol came from Biosolve BV (Valkenswaard, the Netherlands).
Headspace GC-MS analysis was performed on an Agilent 7890B gas chromatograph equipped with a 5977 single quadrupole mass spectrometer (Agilent Technologies, Amstelveen, the Netherlands) and a multi-purpose sampler (MPS-2, Gerstel, Mühlheimander Ruhr, Germany).
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