Lipids were from Avanti Polar Lipids (Alabaster, AL), Sigma-Aldrich (St. Louis, MO) and Echelon Biosciences (Salt Lake City, UT). Dialysis tubing was from Repligen Corporation (Waltham, MA). GTP, ATP, ADP, ATPγS and Histodenz were from Sigma-Aldrich. BioBeads-SM2 were purchased from BioRad (Hercules, CA), and βoctylglucoside was from Anatrace (Maumee, OH). Cy5-Streptavidin was from SeraCare (Milford, MA). Biotinconjugated R-phycoerythrin was from Life Technologies Corporation (Eugene, OR) and unlabeled streptavidin was from Pierce Thermo Scientific (Rockford, IL). MARCKS Effector Domain peptide was from Vivitide (Gardner, MA). Proteins R (Nyv1), Qa (Vam3), Qb (Vti1), and Qc (Vam7; Mima et al., 2008; Schwartz and Merz, 2009) , sQb (Zick and Wickner, 2013) , Sec17 (Schwartz and Merz, 2009) , Sec18 (Haas and Wickner, 1996) , Ypt7-tm and GST-Nyv1 (Song et al., 2020) , GST-C1B (Johnson et al., 2000; Fratti et al., 2004) , and HOPS (Zick and Wickner, 2013) were purified as described, frozen in small aliquots in liquid nitrogen, and stored at -80°C.
Biotinconjugated r phycoerythrin
Manufactured by Thermo Fisher Scientific
Biotin-conjugated R-phycoerythrin is a fluorescent dye-protein complex. R-phycoerythrin is a naturally occurring chromophore-containing protein derived from red algae. When conjugated to biotin, it can be used as a labeling reagent for various biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Histodenz, by Merck Group (2 mentions)
Lipids, by Avanti Polar Lipids (2 mentions)
Dialysis tubing, by Repligen (2 mentions)
Atpγs, by Merck Group (1 mentions)
Cy5 streptavidin, by LGC (1 mentions)
β octylglucoside, by Anatrace (1 mentions)
Adenosine diphosphate (adp), by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Bio beads sm 2, by Bio-Rad (1 mentions)
Pi 3 p, by Echelon Biosciences (1 mentions)
Pierce protein a magnetic beads, by Thermo Fisher Scientific (1 mentions)
Bio beads sm 2 resin, by Bio-Rad (1 mentions)
2 protocols using biotinconjugated r phycoerythrin
1
Purification and Characterization of SNARE Proteins
2
Lipid Reagent Procurement and Handling
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Lipids were purchased from Avanti Polar Lipids (Alabaster, AL) with the exceptions of PI3P (p hosphatidylinositol 3-phosphate diC16) from Echelon Biosciences (Salt Lake City, UT) and ergosterol from Fluka (St. Louis, MO). Octylglucoside (n-octyl-ß-d -glucopyranoside) was from Anatrace (Maumee, OH), Cy5-labeled streptavidin from LGC Clinical Diagnostics (Milford, MA), biotin-conjugated R-phycoerythrin from Life Technologies Corporation (Eugene, OR), and Histodenz from Sigma-Aldrich (St. Louis, MO). Dialysis tubing was from Repligen Corporation (Waltham, MA) and Bio-Beads SM-2 Resin from Bio-Rad Laboratories (Hercules, CA). Underivatized streptavidin and Pierce Protein-A Magnetic Beads were from Thermo Scientific (Waltham, MA).
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