Jnm al 400 nmr spectrometer

FABMS was performed using a JMS-MS 700 mass spectrometer (JEOL, Tokyo, Japan). 1 H and 13 C NMR spectra were recorded on a JNM-AL-400 NMR spectrometer (JEOL) at 400 MHz for 1 H and 100 MHz for 13 C in CD 3 OD (δ H 3.31, δ C 49.15). Optical rotations were measured with a JASCO P-2300 digital polarimeter (JASCO, Tokyo, Japan). UV spectra were obtained on a Hitachi U-3310 UV-Visible spectrophotometer (Hitachi, Tokyo, Japan) and IR spectra on a Perkin-Elmer Spectrum One Fourier transform infrared spectrometer (Perkin-Elmer, Waltham, MA, USA). CD spectra were measured with a spectrometer (J-720; JASCO). Secondary metabolites were analyzed by a LaChrom Elite HTA system (Hitachi). Preparative HPLC was conducted using a Toyosoda CCPU dual pump (Toyosoda Kogyo, Tokyo, Japan) with a Tosoh UV-8010 detector (Tosoh, Tokyo, Japan).

General Experimental Procedures Specific rotations were determined with a JASCO P-2300 digital polarimeter. UV spectra were measured on a U-3310 UV-Visible spectrophotometer (Hitachi) and IR spectra on a PerkinElmer, Inc. Spectrum One Fourier transform infrared spectrometer. ECD spectra were measured with a JASCO J-720 spectrometer. NMR spectra were recorded on a JEOL JNM-AL-400 NMR spectrometer (400 MHz for 1 H and 100 MHz for 13 C) in CDCl 3 (δ H 7.24, δ C 77.0). FAB-MS and HR-FAB-MS were performed using a JMS-MS 700 mass spectrometer (JEOL). Preparative HPLC was carried out with a Hitachi L-6200 system.
Materials PTP1B was purchased from Enzo Life Sciences

General Experimental Procedures Specific rotations were assessed with a JASCO P-2300 digital polarimeter (JASCO, Ltd., Tokyo, Japan). UV spectra were measured on a U-3310 UV-Vis spectrophotometer (Hitachi, Ltd., Tokyo, Japan) and IR spectra on a PerkinElmer, Inc. Spectrum One Fourier transform infrared spectrometer (PerkinElmer, Inc., Waltham, MA, U.S.A.). ECD spectra were measured with a J-720 spectrometer (JASCO). NMR spectra were recorded on a JEOL JNM-AL-400 NMR spectrometer (400 MHz for 1 H and 100 MHz for 13 C) in CDCl 3 (δ H 7.24, δ C 77.0). FAB-MS and HR-FAB-MS were performed using a JMS-MS 700 mass spectrometer (JEOL, Tokyo, Japan). Preparative HPLC was performed with a Hitachi L-6200 system (Hitachi, Ltd.).
Materials Middlebook 7H9 broth, polysorbate 80, and Middlebook OADC were purchased from BD (Franklin Lakes, NJ, U.S.A.). All other chemicals including organic solvents were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). ). 1 (link) H-and 13 C-NMR (CDCl 3 ), see Table 1.

General Experimental Procedures EI-MS and FAB-MS were measured on a JMS-MS 700 mass spectrometer (JEOL, Tokyo, Japan). 1 (link) H-and 13 C-NMR spectra were recorded on a JNM-AL-400 NMR spectrometer (JEOL) at 400 MHz for 1 H and 100 MHz for 13 (link) C in CDCl 3 (δ H 7.24, δ C 77.0). Specific rotations were obtained with the digital polarimeter P-2300 (JASCO, Tokyo, Japan). UV spectra were measured on the UV-visible (Vis) spectrophotometer U-3310 (Hitachi High Technologies Co., Ltd., Tokyo, Japan). ECD spectra were measured with a JASCO J-720 spectropolarimeter. IR spectra were recorded on the Fourier transform infrared spectrometer FT-710 (Horiba Ltd., Kyoto, Japan). Preparative HPLC was performed using an L-6200 HPLC system (Hitachi High Technologies Co., Ltd.).
Materials PTP1B was purchased from Enzo Life Sciences (Farmingdale, NY, U.S.A.). p-Nitrophenyl phosphate (pNPP) was purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). Oleanolic acid was purchased from Tokyo Chemical Industry (Tokyo, Japan). Plastic plates (96-well) were purchased from Corning Inc. (Corning, NY, U.S.A.). All other chemicals including organic solvents were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).