For in vitro studies, we used three categories of breast cell lines. For Luminal A (LumA) phenotype we used T47D (from ICLC cell biobank, Genova, It), for luminal B (LumB) we used the BT474 (kindly provided by Dr. Daniela Gaglio, IBFM-CNR), for HER2+ phenotype we used SKBR3 (ATCC, Manassas, Virginia, USA), and lastly, for TNBC phenotype we used MDA-MB-231 (ICLC). These cell lines have been selected as representative of the BC subtypes, as reported in [31 (link)]. MCF-10 (ATCC) cell line was used as control, being representative of a non-tumorigenic epithelial cell line. T47D cells were cultured in High Glucose DMEM (Gibco, Life Technologies, Carlsbad, California, Stati Uniti); SKBR3 and BT-474 in RPMI (Euroclone, Italy) and MDA-MB-231 and MCF-10 in Advanced DMEM (Euroclone, Italy). All of the media were supplemented with 10% heat-inactivated foetal bovine serum, penicillin and streptomycin (50 IU/mL), and 2 mM glutamine (all Euroclone, Italy). The cells were maintained in a humidified atmosphere of 5% CO2 at 37 °C.
Mcf 10
Manufactured by Euroclone
Sourced in Italy
The MCF-10 is a cell culture system designed for the growth and maintenance of non-transformed human breast epithelial cells. It provides a standardized and well-characterized in vitro model for the study of normal mammary cell biology.
Automatically generated - may contain errors
Lab products found in correlation
Skbr3, by American Type Culture Collection (2 mentions)
Mda mb 231, by American Type Culture Collection (2 mentions)
High glucose dmem, by Thermo Fisher Scientific (2 mentions)
Roswell park memorial institute (rpmi), by Euroclone (2 mentions)
Glutamine, by Euroclone (2 mentions)
Advanced dmem, by Euroclone (2 mentions)
Foetal bovine serum (fbs), by Euroclone (2 mentions)
Mcf 10, by American Type Culture Collection (2 mentions)
Penicillin, by Euroclone (2 mentions)
Streptomycin, by Euroclone (2 mentions)
Mda mb 231, by Euroclone (2 mentions)
Bt474, by Euroclone (2 mentions)
Skbr3, by Euroclone (2 mentions)
2 protocols using mcf 10
1
Breast Cancer Cell Line Characterization
2
Breast Cancer Cell Line Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For in vitro studies, we used three categories of breast cell lines. For Luminal A (LumA) phenotype we used T47D (from ICLC cell biobank, Genova, It), for luminal B (LumB) we used the BT474 (kindly provided by Dr. Daniela Gaglio, IBFM-CNR), for HER2+ phenotype we used SKBR3 (ATCC, Manassas, Virginia, USA), and lastly, for TNBC phenotype we used MDA-MB-231 (ICLC). These cell lines have been selected as representative of the BC subtypes, as reported in [31 (link)]. MCF-10 (ATCC) cell line was used as control, being representative of a non-tumorigenic epithelial cell line. T47D cells were cultured in High Glucose DMEM (Gibco, Life Technologies, Carlsbad, California, Stati Uniti); SKBR3 and BT-474 in RPMI (Euroclone, Italy) and MDA-MB-231 and MCF-10 in Advanced DMEM (Euroclone, Italy). All of the media were supplemented with 10% heat-inactivated foetal bovine serum, penicillin and streptomycin (50 IU/mL), and 2 mM glutamine (all Euroclone, Italy). The cells were maintained in a humidified atmosphere of 5% CO2 at 37 °C.
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