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2 protocols using anti mbp antibody

1

Antibody Reagents for DNA Repair Analysis

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The NUDT16 antibody was purchased from Proteintech Group. The anti-phospho-RPA2 (S4/S8) was obtained from Bethyl Laboratories. The anti-FLAG M2 antibody was purchased from Sigma. Anti-PARP1 and anti-GAPDH antibodies were obtained from Santa Cruz. Anti-PAR and anti-PARP1 antibodies were purchased from Trevigen. The anti-RPA32 and anti-ubiquitin antibodies were purchased from Abcam. The anti-KU70, anti-KU80, anti-Myc and anti-α-Tubulin antibodies were obtained from Cell Signaling Technology. The anti-CtIP antibody was purchased from Active motif. The anti-MBP antibody was purchased from BioLegend.
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2

Comprehensive Antibody Catalog for DNA Damage Research

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The 53BP1 antibody we generated was described previously (11 (link),15 (link)). The anti-FLAG M2 (#F3165), anti-RPA32 (#SAB1406400), anti-γH2AX (#05-636), anti-Histone H3 (#04-928), and anti-β-actin (#A5411) antibodies were purchased from MilliporeSigma. The anti-HDGFRP3 antibody was obtained from Assay Biotech (#C30644) and Proteintech (#12380-1-AP). The anti-α tubulin antibody (#2144S), anti-H2AX (#2595S), anti-GST antibody (#2625S), anti-H4K20me1 antibody (#9724SS), anti-H4K20me3 antibody (#5737S), anti-PP2A antibody sample kit (#9780T) and anti-53BP1 antibody (#4937S) were obtained from Cell Signaling Technology. The anti-CtIP antibody (#61141) was purchased from Active motif. The anti-GAPDH antibody (#sc-47724) and anti-53BP1 antibody (#sc-517281) were obtained from Santa Cruz. The anti-MBP antibody (#906901), anti-Rat 53BP1 antibody (#933002), anti-Rat Flag antibody (#637301), Alexa Fluor® 488 anti-PCNA (#307909), and Alexa Fluor® 488 anti-γH2AX (#613405) were purchased from BioLegend. The anti-HA antibody (#PI26183) was obtained from Thermo Fisher Scientific. The anti-H4K20me2 antibody was purchased from Diagenode (#C15200220).
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