Anti mlkl clone 3h1

Manufactured by Merck Group

Anti-MLKL clone 3H1 is a laboratory reagent used for the detection of MLKL (mixed lineage kinase domain-like protein) in biological samples. MLKL is a key executioner of necroptosis, a form of programmed cell death. The Anti-MLKL clone 3H1 antibody can be used in various analytical techniques, such as Western blotting, to identify and quantify MLKL levels in cells or tissues.

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Lab products found in correlation

Actinomycin d, by Merck Group (2 mentions) Ap20187, by Takara Bio (2 mentions) Poly 1 c, by InvivoGen (2 mentions) Gsk 872, by Aobious (2 mentions) Anti caspase 8 1g12, by Enzo Life Sciences (2 mentions) Anti ripk1 clone 38, by BD (2 mentions) Cytotox96 ldh release kit, by Promega (2 mentions) Anti drp1 antibody, by BD (1 mentions) Anti il 1β, by GeneTex (1 mentions) Ultrapure lps, by InvivoGen (1 mentions) Pam3csk4, by InvivoGen (1 mentions)

Close spelling variants of this product

Anti-MLKL (15 citations) MLKL (clone 3H1) (2 citations)

2 protocols using anti mlkl clone 3h1

Immunoblotting of Cell Death Pathway

Cited 1 time

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Antibodies against caspase-1, NLRP3 and ASC were made in house and were described previously14 (link)25 (link)51 (link). Anti-DRP1 antibody (Catalogue No. 611738) and anti-RIPK1 clone 38 (Catalogue No. 610459) were from BD biosciences. Anti-caspase-8 1G12 was from Enzo (Catalogue No. ALX-804-447-C100). Anti-MLKL clone 3H1 was from EMD Millipore (Catalogue No. MABC604). Anti-IL-1β was from GeneTex (Catalogue No. GTX74034). Anti-RIPK3 antibody (Catalogue No. R4277), ATP and actinomycin D were obtained from Sigma. Ultrapure LPS, Pam3CSK4 and poly(I:C) were obtained from InvivoGen. zVAD was obtained from ApexBio. GSK'872 was obtained from Aobious. AP20187 was obtained from Clontech. CytoTox96 LDH-release kit was from Promega. All antibodies were used at 1/1,000–1/2,000 dilutions for western blot analyses.

Kang S., Fernandes-Alnemri T., Rogers C., Mayes L., Wang Y., Dillon C., Roback L., Kaiser W., Oberst A., Sagara J., Fitzgerald K.A., Green D.R., Zhang J., Mocarski E.S, & Alnemri E.S. (2015). Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3. Nature Communications, 6, 7515.

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Antibodies for Inflammasome and Cell Death

Cited 1 time

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Antibodies against caspase-1, NLRP3 and ASC were made in house and were described previously ^{14 (link), 25 (link), 51 (link)}. Anti-DRP1 antibody (Catalog No. 611738) and anti-RIPK1 clone 38 (Catalog No. 610459) were from BD biosciences. Anti-caspase-8 1G12 was from Enzo (Catalog No. ALX-804-447-C100). Anti-MLKL clone 3H1 was from EMD Millipore (Catalog No. MABC604). Anti-RIPK3 antibody (Catalog No. R4277), ATP and actinomycin D were obtained from Sigma. Ultrapure LPS, Pam3CSK4 and poly(I:C) were obtained from InvivoGen. zVAD was obtained from ApexBio. GSK’872 was obtained from Aobious. AP20187 was obtained from Clontech. CytoTox96 LDH-release kit was from Promega. All antibodies were used at 1/1000–1/2000 dilutions for western blot analyses.

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