Cell viability was determined using CellTiter-Glo® 2D or 3D cell viability assays (#G9242, #G9682, Promega). 500 cells/25 µl were seeded in flat bottom (#781,091, Greiner bio-one) or U-low adhesion (#4516, Corning) 384-well plate, 24 h prior to treatment. Increasing concentrations of compounds are deposited on cells using a HP Digital Drug Dispenser with DMSO total volume normalization. After 48 h, the CellTiter-Glo® 2D or 3D reagent was added (volume/volume) following manufacturer’s instructions. Plates were incubated at RT (room temperature) under agitation for 30 min and luminescence representing the number of viable cells was quantified with a Cytation 3 imaging reader (BioTek®). Experiments were performed independently three times with three technical replicas each.
Celltiter glo 2d or 3d
Manufactured by Promega
CellTiter-Glo® 2D or 3D is a luminescence-based assay that quantifies the amount of ATP present in cell cultures. The assay measures the metabolic activity of cells and can be used to assess cell viability and cytotoxicity in both two-dimensional (2D) and three-dimensional (3D) cell culture models.
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Lab products found in correlation
2 protocols using celltiter glo 2d or 3d
1
Cell Viability Assay for 2D and 3D Cultures
2
Cell Viability Assay with CellTiter-Glo
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Cell viability was determined using CellTiter-Glo® 2D or 3D cell viability assays (#G9242, #G9682, Promega). 500 cells/25 µl were seeded in at bottom (#781091, Greiner bio-one) or U-low adhesion (#4516, Corning) 384-well plate, 24 h prior to treatment. Increasing concentrations of compounds are deposited on cells using a HP Digital Drug Dispenser with DMSO total volume normalization. After 48 h, the CellTiter-Glo® 2D or 3D reagent was added (volume/volume) following manufacturer's instructions.
Plates were incubated at RT (room temperature) under agitation for 30 min and luminescence representing the number of viable cells was quanti ed with a Cytation 3 imaging reader (BioTek®).
Experiments were performed independently three times with three technical replicas each.
Plates were incubated at RT (room temperature) under agitation for 30 min and luminescence representing the number of viable cells was quanti ed with a Cytation 3 imaging reader (BioTek®).
Experiments were performed independently three times with three technical replicas each.
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