Texas green 5 dctp

Manufactured by Thermo Fisher Scientific

Sourced in China

Texas Green-5-dCTP is a fluorescently labeled nucleotide used in various molecular biology applications. It can be incorporated into DNA during synthesis or labeling reactions, enabling the detection and visualization of DNA molecules.

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Lab products found in correlation

Texas red 5 dutp, by Thermo Fisher Scientific (2 mentions)

2 protocols using texas green 5 dctp

Fluorescence in situ Hybridization and Multicolor GISH for Chromosome Analysis

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Fluorescence in situ hybridization was conducted using synthetic oligonucleotide probes as described by Tang et al. (2014 (link)) and Zhao et al. (2016 (link)). The probes Oligo-pSc119.2 (green) and Oligo-pTa535 (red) were synthesized and labeled with FAM or TAMRA by Shanghai Invitrogen Biotechnology Co. Ltd (Shanghai, China) and used for FISH as described by Tang et al. (2014 (link)).
For mc-GISH analysis, XM943 DNA was labeled with Texas Red-5-dUTP (Invitrogen) and SY41 DNA was labeled with Texas Green-5-dCTP (Invitrogen) (2014). SY183 DNA was used as the blocker. Chromosomes were counterstained with 4, 6-diamidino-2-phenylindole (DAPI). Fluorescent signals were scanned and photographed with an Olympus BX53 microscope equipped with a Photometrics SenSys CCD DP80 camera (Olympus, Tokyo, Japan). (Wang et al. 2016a (link), 2016b (link)).

Wang Y., Wang S., Jia X., Tian Z., Wang Y., Wang C., Zhang H., Liu X., Zhao J., Deng P, & Ji W. (2021). Chromosome karyotype and stability of new synthetic hexaploid wheat. Molecular Breeding : New Strategies in Plant Improvement, 41(10), 60.

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Multicolor FISH and GISH Analysis

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Fluorescence in situ hybridization (FISH) was completed using synthetic oligonucleotide probes as described by Tang et al. (2014) and Zhao et al. (2016) . Probes Oligo-pSc119.2 (green) and Oligo-pTa535 (red) were synthesized and labelled with FAM or TAMRA by Shanghai Invitrogen Biotechnology Co. Ltd.
were used for FISH as described by Tang et al. (2014) .
For the multicolor-GISH (mc-GISH) analysis, Triticum urartu Thum. DNA was labelled with Texas Red-5-dUTP (Invitrogen) and Ae. tauschii Coss. DNA was labeled with Texas Green-5-dCTP (Invitrogen) (2014). Ae. Speltoides DNA was blocker. Chromosomes were counterstained with 4,6-diamidino-2-phenylindole (DAPI). Fluorescent signals were scanned and photographed with an Olympus BX53 microscope equipped with a Photometrics SenSys CCD DP80 camera (Japan). (Wang et al. 2016) .

Wang Y., Wang S., Jia X., Tian Z., Wang Y., Wang C., Zhang H., Liu X., Zhao J., Deng P., & Ji W. (2021). Chromosome Karyotype and Stability Identification of New Synthetic Hexaploid Wheat.

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