Cells were seeded into 96-well plates and treated with 1 µg/mL Porphyromonas gingivalis lipopolysaccharide (LPS-Pg; InvivoGen, San Diego, CA, USA), 20 µM of the pan-caspase inhibitor, zVAD (Promega, Madison, WI, USA) or 30 µM Nec-1 (Cambridge Bioscience, Cambridge, UK) for 24 h. Cell survival was determined using the CellTiter-Glo luminescent cell viability assay (Promega). Luminescence was read using an In nite M200 microplate reader (Tecan, Zürich, Switzerland).
In nite m200 microplate reader
Manufactured by Tecan
Sourced in Switzerland
The Infinite M200 microplate reader is a versatile instrument designed for sensitive and accurate absorbance measurements in microplates. It features a xenon flash lamp, a monochromator for wavelength selection, and a high-precision photomultiplier tube detector. The device supports a wide range of wavelengths from 200 to 1000 nm, allowing for various absorbance-based applications.
Automatically generated - may contain errors
Lab products found in correlation
Porphyromonas gingivalis lipopolysaccharide lps pg, by InvivoGen (2 mentions)
Nec 1, by Cambridge Bioscience (2 mentions)
Celltiter glo luminescent cell viability assay, by Promega (2 mentions)
Cell counting kit 8 assay kit, by Selleck Chemicals (1 mentions)
Fitc dextran 4, by Merck Group (1 mentions)
4 protocols using in nite m200 microplate reader
1
Investigating Cell Viability with LPS-Pg, zVAD, and Nec-1
2
Doxorubicin-Induced Cell Viability Assay
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HL-1 cells were seeded into 96-well culture plates with 5000 cells/well and treated with DOX at 5 μM for 9 h. We utilized the Cell Counting Kit-8 assay kit (CCK8, Bimake,China) to measure cell viability according to the manufacturer's instructions. We examined the Optical density (OD) values at 450 nm by an In nite™ M200 Microplate reader (Tecan, Mannedorf, Switzerland) [23] .
3
Investigating Cell Viability with LPS-Pg, zVAD, and Nec-1
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Cells were seeded into 96-well plates and treated with 1 µg/mL Porphyromonas gingivalis lipopolysaccharide (LPS-Pg; InvivoGen, San Diego, CA, USA), 20 µM of the pan-caspase inhibitor, zVAD (Promega, Madison, WI, USA) or 30 µM Nec-1 (Cambridge Bioscience, Cambridge, UK) for 24 h. Cell survival was determined using the CellTiter-Glo luminescent cell viability assay (Promega). Luminescence was read using an In nite M200 microplate reader (Tecan, Zürich, Switzerland).
4
In Vivo Intestinal Permeability Assessment
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In vivo intestinal permeability was assessed using uorescein dextran (FITC-Dextran 4, Sigma-Aldrich) as previously described [14] . Mice were orally gavaged with 0.75 mg/g body weight of 4 kDa FITC-labeled dextran and blood samples were obtained from the retro-orbital venous plexus 5 h after this administration. Blood samples were centrifuged for 10 min at 5000 rpm and plasma was taken and frozen at -20 °C and analyzed the following day. Intestinal permeability to 4 kDa FITC-labeled dextran was determined by measuring the uorescence intensity in plasma at 485 nm/525 nm using an automatic In nite M200 microplate reader (Tecan, Lyon, France).
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