All proteins were resolved in 7.5% SDS-PAGE gels, except Sgs1 (6% SDS-PAGE gel), Rmi1 (10% SDS-PAGE gel), and histone H3 (15% SDS-PAGE gel). Proteins were transferred to polyvinylidene fluoride (PVDF) membranes using the TE70X semidry blotter system (Hoefer). The antibodies used were anti-HA (Roche, 3F10), anti-H3 (Abcam, ab1791), anti-myc (Roche, 9E10), anti-PGK1 (Thermo Scientific, 459250), anti-RFA (Agrisera, AS07 214), and anti-SMT3 (Abcam, ab14405). Blots were incubated with the ECL Prime Western blotting detection reagent (GE Healthcare) followed by exposure to high-performance chemiluminescence films (Amersham Hyperfilm ECL, GE Healthcare) to detect the signal.
As07 214
Manufactured by Abcam
AS07 214 is a lab equipment product offered by Abcam. It serves as a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Ab14405, by Abcam (1 mentions)
Amersham hyperfilm ecl, by GE Healthcare (1 mentions)
Ab1791, by Roche (1 mentions)
Te70x semidry blotter system, by Hoefer (1 mentions)
Ecl prime western blotting detection reagent, by GE Healthcare (1 mentions)
Ab113687, by Abcam (1 mentions)
Icp0380, by Agrisera (1 mentions)
2 protocols using as07 214
1
Western Blot Analysis of Protein Complexes
2
Acetyl-Lysine Immunoprecipitation in Yeast
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunoprecipitation with acetyl-lysine antibody was performed essentially as described previously [5] (link). Cells were grown in YPD until OD600 around 0.5 followed by addition of rapamycin (1ug/ml final) and DMSO or MMS (0.05% final) for 3hrs. Antibody used were acetyl-lysine (ImmuneChem, ICP0380), RPA (Agrisera, AS 07-214) and Pgk1 (Abcam, ab113687).
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