Acetylcholine iodide ach

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Acetylcholine iodide (ACh) is a chemical compound used as a laboratory reagent. It is the iodide salt of the neurotransmitter acetylcholine. ACh is commonly used in research and experiments involving the study of cholinergic systems and their functions.

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Acetylcholine (401 citations) Acetylcholine (ACh) (67 citations) Acetylcholine iodide (50 citations)

2 protocols using acetylcholine iodide ach

Acetylcholine and Thymol Pharmacological Protocol

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Acetylcholine iodide (ACh; Sigma–Aldrich, St. Louis, United States, Lot # MKCK 7580, https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A7000-BULKMKCK7580.pdf) with the purity ≥97% was dissolved in Tyrode or De Jalon solution (depending on the organ used) to achieve appropriate concentrations. Thymol (C₁₀H₁₄O) (Sigma–Aldrich, St. Louis, United States, Lot # SLBZ 9699, https://www.sigmaaldrich.com/specification-sheets/399/216/T0501-BULK.pdf) with the purity ≥98.5% was first dissolved in ethanol at a concentration of 50 mg/ml and then diluted to the selected concentration with Tyrode or De Jalon solution before starting the experiment.

Premrov Bajuk B., Prem L., Vake T., Žnidaršič N, & Snoj T. (2022). The effect of thymol on acetylcholine-induced contractions of the rat ileum and uterus under ex vivo conditions. Frontiers in Pharmacology, 13, 990654.

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Radioligand Binding Assay Protocols

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The radioisotopes [³H] Dopamine (7,8-³H at 20–40 Ci/mmol), carrier-free ⁸⁶RbCl (initial specific activity 13.6–18.5 Ci/μg), and [¹²⁵I]-epibatidine (2200 Ci/mmol) were purchased from Perkin Elmer (Waltham, MA). α-Conotoxin MII (α-CtxMII) was generously provided by J. Michael McIntosh, University of Utah, Salt Lake City, UT. NaCl, KCl, CaCl₂, MgSO₄, glucose, sucrose, HEPES, tetrodotoxin, (−) nicotine bitartrate, nicotine freebase, acetylcholine iodide (ACh), diisopropyl fluorophosphate (DFP), polyethelenimine, bovine serum albumin (BSA), and cytisine were purchased from Sigma Chemical Co (St. Louis, MO).

O’Neill H.C., Wageman C.R., Sherman S.E., Grady S.R., Marks M.J, & Stitzel J.A. (2018). The interaction of the Chrna5 D398N variant with developmental nicotine exposure. Genes, brain, and behavior, 17(7), e12474.

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