Bactec plus aerobic f culture

The environmental strains were cultured on Müller Hinton agar before the analysis. Clinical strains were obtained from positive blood cultures at the clinic. Reference strains were inoculated blood culture bottles (BD Bactec Plus Aerobic/F Culture and BD Bactec Plus Anaerobic Lytic/F) and incubated in an automated BACTEC FXTM blood culture system (Becton Dickinson, Franklin Lakes, NJ) until agged as positive. The preparation of the bacteria was as described in Axelsson et al., (2019) (9) . Brie y, 1 ml blood from the aerobic bottles were added to 200 µl Saponin (5%) and 1 ml of the mixture was then added to 200 µl lysis buffer (MALDI Sepsityper KIT, Bruker, Bremen, Germany). The preparation from the anaerobic bottles were treated the same except that no saponin was added. The samples were subsequently centrifuged at 13 000 x g for two minutes and the supernatant removed. The pellet was resuspended in 1 ml sterile MQ water. After an additional centrifugation at 13 000 x g for two minutes, the supernatant was removed and the pellet resuspended in Müller Hinton Cation Adjusted Broth (MHCA, Sigma-Aldrich, USA) to McFarland 1,0 before the analysis.

The environmental strains were cultured on Müller Hinton agar before the analysis. Clinical strains were obtained from positive blood cultures at the clinic. Reference strains were inoculated blood culture bottles (BD Bactec Plus Aerobic/F Culture and BD Bactec Plus Anaerobic Lytic/F) and incubated in an automated BACTEC FXTM blood culture system (Becton Dickinson, Franklin Lakes, NJ) until agged as positive. The preparation of the bacteria was as described in Axelsson et al., (2019) (9) . Brie y, 1 ml blood from the aerobic bottles were added to 200 µl Saponin (5%) and 1 ml of the mixture was then added to 200 µl lysis buffer (MALDI Sepsityper KIT, Bruker, Bremen, Germany). The preparation from the anaerobic bottles were treated the same except that no saponin was added. The samples were subsequently centrifuged at 13 000 x g for two minutes and the supernatant removed. The pellet was resuspended in 1 ml sterile MQ water. After an additional centrifugation at 13 000 x g for two minutes, the supernatant was removed and the pellet resuspended in Müller Hinton Cation Adjusted Broth (MHCA, Sigma-Aldrich, USA) to McFarland 1,0 before the analysis.