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S2 biodisruptor

Manufactured by Covaris
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The S2 Biodisruptor is a laboratory instrument designed for the disruption and homogenization of biological samples. It utilizes focused acoustic energy to efficiently break down a variety of sample types, including cells, tissues, and other materials, to facilitate downstream analytical processes.

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Lab products found in correlation

Hiseq 2500, by Illumina (3 mentions) Spri bead, by Beckman Coulter (2 mentions) Hiseq x ten, by Illumina (1 mentions) Truseq nano, by Illumina (1 mentions)

3 protocols using s2 biodisruptor

1

Rapid Whole Genome Sequencing Protocols

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Genomic DNA was prepared for WGS using either Illumina TruSeq PCR-Free (rapid WGS) or TruSeq Nano (HiSeq X Ten) sample preparation according to the manufacturer’s protocols. Briefly, 500 ng of DNA was sheared with a Covaris S2 Biodisruptor, end-repaired, A-tailed, and adapter-ligated. Quantitation was carried out by real-time PCR. Libraries were sequenced by Illumina HiSeq 2500 instruments (2 × 100 nt) in rapid-run mode or by HiSeq X Ten (2 × 150 nt).
Soden S.E., Saunders C.J., Willig L.K., Farrow E.G., Smith L.D., Petrikin J.E., LePichon J.B., Miller N.A., Thiffault I., Dinwiddie D.L., Twist G., Noll A., Heese B.A., Zellmer L., Atherton A.M., Abdelmoity A.T., Safina N., Nyp S.S., Zuccarelli B., Larson I.A., Modrcin A., Herd S., Creed M., Ye Z., Yuan X., Brodsky R.A, & Kingsmore S.F. (2014). Effectiveness of exome and genome sequencing guided by acuity of illness for diagnosis of neurodevelopmental disorders. Science translational medicine, 6(265), 265ra168.
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2

Whole Genome Sequencing Library Prep

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Isolated genomic DNA was prepared for WGS with a modification of the Illumina TruSeq sample preparation4 ,13 . Briefly, 500 ng of DNA was sheared with a Covaris S2 Biodisruptor, end-repaired, A-tailed, and adaptor-ligated. Polymerase chain reaction (PCR) was omitted. Libraries were purified with SPRI beads (Beckman Coulter). Quantitation was carried out by real-time PCR. Libraries were denatured with 0.1 M NaOH and diluted to 2.8 pM in hybridization buffer. Samples were each loaded onto two flowcells, followed by 2 × 100 cycle sequencing on Illumina HiSeq 2500 instruments.
Guimier A., Gabriel G.C., Bajolle F., Tsang M., Liu H., Noll A., Schwartz M., El Malti R., Smith L.D., Klena N.T., Jimenez G., Miller N.A., Oufadem M., Moreau de Bellaing A., Yagi H., Saunders C.J., Baker C.N., Di Filippo S., Peterson K.A., Thiffault I., Bole-Feysot C., Cooley L.D., Farrow E.G., Masson C., Schoen P., Deleuze J.F., Nitschké P., Lyonnet S., de Pontual L., Murray S.A., Bonnet D., Kingsmore S.F., Amiel J., Bouvagnet P., Lo C.W, & Gordon C.T. (2015). MMP21 is mutated in human heterotaxy and is required for normal left-right asymmetry in vertebrates. Nature genetics, 47(11), 1260-1263.
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3

Whole Genome Sequencing Library Prep

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated genomic DNA was prepared for WGS with a modification of the Illumina TruSeq sample preparation4 ,13 . Briefly, 500 ng of DNA was sheared with a Covaris S2 Biodisruptor, end-repaired, A-tailed, and adaptor-ligated. Polymerase chain reaction (PCR) was omitted. Libraries were purified with SPRI beads (Beckman Coulter). Quantitation was carried out by real-time PCR. Libraries were denatured with 0.1 M NaOH and diluted to 2.8 pM in hybridization buffer. Samples were each loaded onto two flowcells, followed by 2 × 100 cycle sequencing on Illumina HiSeq 2500 instruments.
Guimier A., Gabriel G.C., Bajolle F., Tsang M., Liu H., Noll A., Schwartz M., El Malti R., Smith L.D., Klena N.T., Jimenez G., Miller N.A., Oufadem M., Moreau de Bellaing A., Yagi H., Saunders C.J., Baker C.N., Di Filippo S., Peterson K.A., Thiffault I., Bole-Feysot C., Cooley L.D., Farrow E.G., Masson C., Schoen P., Deleuze J.F., Nitschké P., Lyonnet S., de Pontual L., Murray S.A., Bonnet D., Kingsmore S.F., Amiel J., Bouvagnet P., Lo C.W, & Gordon C.T. (2015). MMP21 is mutated in human heterotaxy and is required for normal left-right asymmetry in vertebrates. Nature genetics, 47(11), 1260-1263.
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