Pure MYR (1 mg) and different ratio formulations of MyNF (containing 1 mg MYR) were dissolved in 1 mL deionized water and shaken (Vortex-Gene 2, Scientific Industries, Bohemia, NY, USA) for 10 min, respectively. Subsequently, each sample was filtered with a 0.45 µm syringe filter (13 mm Acrodisc® syringe filters with GHP membrane, Pall Corporation, Port Washington, NY, USA) and then diluted 100-fold with pure water before HPLC analysis, after which the MYR concentration of each sample was calculated by the MYR standard curve.
Acrodisc syringe filters with ghp membrane
Manufactured by Pall Corporation
Sourced in United States
Acrodisc® syringe filters with GHP membrane are a type of lab equipment used for filtration purposes. They feature a hydrophilic GHP membrane that is designed to remove particulates and clarify samples. The filters are available in a range of pore sizes to suit different filtration needs.
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5 protocols using acrodisc syringe filters with ghp membrane
1
MYR Quantification in Formulations
2
Solubility Determination of Morin and Liposomal Formulation
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Measuring the solubility of morin and its liposomal formulation is the primary step towards preparing an appropriate concentration for further experiments. Specifically, 1 mg of free morin and liposomal morin that contains equal amounts of morin were dissolved in water (at room temperature) under a vigorous vortex (Gene 2, Scientific Industries, Bohemia, NY, USA) for uniform mixing. Solutions were subsequently filtered through a 0.45 µm syringe filter (13 mm Acrodisc® syringe filters with GHP membrane, Pall Corporation, Port Washington, NY, USA) prior to being ten-fold diluted for HPLC analysis. The above-mentioned standard curve was employed to determine the concentrations of samples and then calculate the aqueous solubility of morin in free form and in liposomal formulations.
3
Solubility Enhancement of 734THI via PVP K30
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Phase solubility studies were performed in compliance with the method of Higuchi and Connors.24 In brief, an excess amount of raw 734THI was added to 1 mL of pure water containing different concentrations of PVP K30 (ranging from 1 to 27 mg). The suspensions were vigorously shaken for 24 h at room temperature using a vibrator (Vortex-Gene 2, Scientific Industries, Inc., NY, USA), and then the solution was filtered through a 0.45 μm syringe filter (13 mm Acrodisc® syringe filters with GHP membrane; Pall Corporation, NY, USA). The concentration of 734THI in each sample was analyzed by the HPLC method. The stability constants (Ks) were calculated according to the slope of the straight portion of the phase solubility diagram. Equation 1 is as follows:
where S0 is the 734THI solubility in pure water.
where S0 is the 734THI solubility in pure water.
4
Evaluating 7HP Solubility and Yield
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For yield, different ratios of 7HP were dissolved in methanol. 734THIF concentrations of all samples were determined by HPLC, performed in triplicate, and calculated as follow: Yield (%) = 734THIF weight (mg)/theoretical amount of 734THIF (mg) × 100%.
For water solubility evaluation, different ratios of 7HP were dissolved in water and then shaken for 1 h by Vortex-Gene 2 (Scientific Industries, Bohemia, NY, USA). All samples were filtered with 0.45 μm syringe filter (13 mm Acrodisc® syringe filters with GHP membrane; Pall Corporation, NY, USA) before analysis by HPLC.
For water solubility evaluation, different ratios of 7HP were dissolved in water and then shaken for 1 h by Vortex-Gene 2 (Scientific Industries, Bohemia, NY, USA). All samples were filtered with 0.45 μm syringe filter (13 mm Acrodisc® syringe filters with GHP membrane; Pall Corporation, NY, USA) before analysis by HPLC.
5
Solubility Assay for ACM and AHP
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In brief, 1 mg of raw ACM and AHP (corresponding to 1 mg of ACM) was added to 1 mL of distilled water. Each sample was vigorously shaken with a mixer (Vortex-Gene 2, Scientific Industries, Bohemia, NY, USA) for 10 min and then filtered with a 0.45 μm syringe filter (13 mm Acrodisc® syringe filters with GHP membrane; Pall Corporation, Port Washington, NY, USA)). All the samples were analyzed by the HPLC method as described above, and then the solubility of ACM was calculated.
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