Olodaterol hydrochloride

Ovalbumin (Serva Electrophoresis GmbH, Heidelberg, Germany) was dissolved and diluted in phosphate buffered saline (PBS; Lonza, Verviers, Belgium) for the immunization, and in 0.9% saline (Delta Select GmbH, Dreieich, Germany) for the OVA challenge. Aluminium hydroxide (ImjectAlum; ThermoScientific, Rockford, IL, USA) was dissolved and diluted in PBS. Citric acid monohydrate and pyrilamine maleate were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany) and dissolved and diluted in 0.9% saline. Respitose (Lactose monohydrate, ML003) was from Boehringer Ingelheim (Ingelheim, Germany). Codeine phosphate hemihydrate was purchased from Merck Company (Germany). Olodaterol hydrochloride, salmeterol xinafoate, formoterol fumarate, and indacaterol maleate were synthesized at the Department of Chemical Research, Boehringer Ingelheim Pharma GmbH & Co. KG (Biberach, Germany).

Olodaterol hydrochloride [BI 1744 Cl] was provided by Boehringer Ingelheim Pharma GmbH & Co. KG, Germany. Interleukin (IL-8) ELISA kit [Cat# CHC1303] was purchased from Life Technologies (Carlsbad, CA, USA). Anti-Muc5AC antibody [Cat# ab24071] for immunohistochemistry was purchased from Abcam (Cambridge, United Kingdom). ICI 118,551 hydrochloride [Cat# I127-5MG] was purchased from Sigma Aldrich (St. Louis, MO, US). Butaxamine hydrochloride [B1385-50MG] was also purchased from Sigma Aldrich.