Our microscopic system for in vivo nanoimaging was described in detail in our previous studies (Kobirumaki-Shimozawa et al., 2016 (link); Shimozawa et al., 2017 (link)). In brief, an upright microscope (BX-51WI; Olympus Co.) combined with a Nipkow confocal scanner (CSU21; Yokogawa Electric Co.) and an electron-multiplying charge-coupled device camera (iXonEM+; Andor Technology Ltd.) were used at a 512 × 170–pixel resolution at an exposure time of 9.8 ms. A 60× water immersion lens (LUMPLFLN 60×W, numerical aperture 1.00; Olympus Co.) was used to visualize the LV surface. α-Actinin-AcGFP–expressing myocytes in the left ventricle were excited by a 488-nm laser light (HPU50211-PFS; Furukawa Electric Co.), and the resultant fluorescence signals were detected by using a BA510–550 bandpass emission filter (Olympus Co.).
Nipkow confocal scanner csu21
Manufactured by Yokogawa
Sourced in Japan
The Nipkow confocal scanner (CSU21) is a type of confocal microscope system that uses a spinning Nipkow disk to rapidly scan a sample. The Nipkow disk contains a series of pinholes that allow for the sequential illumination and detection of points within the sample, providing optical sectioning capabilities.
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Lab products found in correlation
Ixonem, by Oxford Instruments (2 mentions)
Bx51wi, by Olympus (2 mentions)
Lumplfln 60 w, by Olympus (1 mentions)
Ba510 550 bandpass emission filter, by Olympus (1 mentions)
Xlfluor 2x 340, by Olympus (1 mentions)
Lumplfln 40xw, by Olympus (1 mentions)
Xlumplfln20xw, by Olympus (1 mentions)
Ba510 550, by Olympus (1 mentions)
Ba575if, by Olympus (1 mentions)
2 protocols using nipkow confocal scanner csu21
1
In Vivo Nanoimaging of Cardiomyocytes
2
In Vivo Nanoimaging of Cardiomyocytes
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The details of the microscopic system for in vivo nanoimaging have been described in our previous studies [5 (link), 6 (link)]. In brief, an upright microscope (BX-51WI, Olympus Co., Tokyo, Japan) combined with a Nipkow confocal scanner (CSU21, Yokogawa Electric Co., Tokyo, Japan) and an electron multiplying CCD (EMCCD) camera (iXonEM+, Andor Technology Ltd, Belfast, Northern Ireland) were used at a 512 × 512 (or 512 × 170) pixel resolution at an exposure time of 28 (or 9.8) ms. A water immersion lens, either 60× (LUMPLFLN 60XW, N/A 1.00, Olympus Co.), 40× (LUMPLFLN 40XW, N/A 0.80, Olympus Co.), or 20× (XLUMPLFLN 20XW, N/A 1.00, Olympus Co.), and also a 2× lens (XLFluor 2X/340, N/A 0.14, Olympus Co.) were used to visualize the LV surface.
AcGFP-expressing myocytes were excited by a 488 nm laser light (HPU50211-PFS, Furukawa Electric Co., Tokyo, Japan), and the resultant fluorescence signals (emission filter: BA510–550, Olympus Co., Tokyo, Japan) were detected. In the experiments with CellMask, the heart was excited at 532 nm (MiniGreen FCIM-100; Snake Creek Lasers, Friendsville, PA, USA), and the resultant fluorescence signals (emission filter: BA575IF, Olympus Co.) were detected. When excited at 532 nm, the wavelength range for the detection of the fluorescence of CellMask was >575 nm.
AcGFP-expressing myocytes were excited by a 488 nm laser light (HPU50211-PFS, Furukawa Electric Co., Tokyo, Japan), and the resultant fluorescence signals (emission filter: BA510–550, Olympus Co., Tokyo, Japan) were detected. In the experiments with CellMask, the heart was excited at 532 nm (MiniGreen FCIM-100; Snake Creek Lasers, Friendsville, PA, USA), and the resultant fluorescence signals (emission filter: BA575IF, Olympus Co.) were detected. When excited at 532 nm, the wavelength range for the detection of the fluorescence of CellMask was >575 nm.
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