The cells collected after 24 h were ≥90% viable. DCs were stained with antibodies against CD11C-PE/BV421, CD86-Percp-Cy5.5, CD80-FITC, and CD40-FITC (BD Bioscience, San Jose, California).
Cd86 percp cy5
The CD86-PerCP-Cy5.5 is a fluorescently-labeled monoclonal antibody that binds to the CD86 surface antigen. CD86 is a co-stimulatory molecule expressed on antigen-presenting cells, and is involved in T cell activation. The PerCP-Cy5.5 fluorescent label allows for detection of CD86-positive cells using flow cytometry.
Lab products found in correlation
5 protocols using cd86 percp cy5
Monocyte-Derived Dendritic Cell Protocol
The cells collected after 24 h were ≥90% viable. DCs were stained with antibodies against CD11C-PE/BV421, CD86-Percp-Cy5.5, CD80-FITC, and CD40-FITC (BD Bioscience, San Jose, California).
Monocyte-Derived Dendritic Cell Activation by HSPs
The endotoxin concentration in HSP60 was checked by LAL Chromogenic Endotoxin Quantitation Kit (Thermofisher Scientific, IL). The level of endotoxin in the HSP60 was ≤1 EU/mg, according to manufacturer information, which is equal to ≤0.1 ng/mg. Our working concentration 5 μg/mL of HSP60 contains ≤0.0005 ng endotoxin. A similar concentration of endotoxin was determined in HSP90.
Multiparameter Flow Cytometry Staining
Multi-parameter Flow Cytometry Analysis
Phenotypic Analysis of Cryopreserved PBMCs
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