Frequency of antigen specific IFN-γ producing T cells in spleen was evaluated using IFN-γ ELISPOT kit (BD Biosciences). Immobilon-P hydrophobic PVDF plates (Millipore) were briefly treated with 35% ethanol, washed 2 times with PBS, and coated overnight with anti-mouse IFN-γ antibody at 4 °C. The following day, plates were blocked with 200 μL of R-10 medium with for 2 h at room temperature (RT). 100,000 splenocytes in R-10 medium were plated in each well, with or without restimulation with 10 μg/mL of SIINFEKL peptide overnight at 37 °C. Spots were then developed following the manufacturer’s instructions.
Immobilon p hydrophobic pvdf plates
Manufactured by Merck Group
Immobilon-P hydrophobic PVDF plates are a type of laboratory equipment used for various applications. They are made of polyvinylidene fluoride (PVDF), a hydrophobic material, and are designed to provide a stable and inert surface for various experimental procedures. The core function of these plates is to offer a controlled and consistent environment for sample preparation, separation, and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using immobilon p hydrophobic pvdf plates
1
ELISPOT Analysis of Antigen-Specific IFN-γ T Cells
2
Antigen-specific IFN-γ T Cell Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The frequency of antigen-specific
IFN-γ producing T cells in the spleen was evaluated using IFN-γ
ELISPOT kit (BD Biosciences). Immobilon-P hydrophobic PVDF plates
(Millipore) were briefly treated with 35% ethanol, washed 2 times
with PBS, and coated overnight with anti-mouse IFN-γ antibody
at 4 °C. The following day, plates were blocked with 200 μL
R-10 medium with for 2 h at room temperature. 100 000 splenocytes
in R-10 medium were plated in each well with or without restimulation
with 10 μg/mL of SIINFEKL peptide overnight at 37 °C. Spots
were then developed following the manufacturer’s instructions.
Unstimulated control showed minimum numbers of spot. Data presented
herein are obtained using n = 4–6 animals
(except for vehicle control in linker length study which was n = 3).
IFN-γ producing T cells in the spleen was evaluated using IFN-γ
ELISPOT kit (BD Biosciences). Immobilon-P hydrophobic PVDF plates
(Millipore) were briefly treated with 35% ethanol, washed 2 times
with PBS, and coated overnight with anti-mouse IFN-γ antibody
at 4 °C. The following day, plates were blocked with 200 μL
R-10 medium with for 2 h at room temperature. 100 000 splenocytes
in R-10 medium were plated in each well with or without restimulation
with 10 μg/mL of SIINFEKL peptide overnight at 37 °C. Spots
were then developed following the manufacturer’s instructions.
Unstimulated control showed minimum numbers of spot. Data presented
herein are obtained using n = 4–6 animals
(except for vehicle control in linker length study which was n = 3).
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