Vibrance antifade mounting medium
Vibrance antifade mounting medium is a laboratory product designed to preserve and protect fluorescent signals in mounted samples. It helps maintain the brightness and stability of fluorescent dyes used in microscopy and other applications.
Lab products found in correlation
4 protocols using vibrance antifade mounting medium
Apoptosis Quantification in FFPE Tissue
Visualization of CDN Uptake in Tumor Spheroids
Quantification of Vascular Endothelial Cadherin in High Endothelial Venules
VE-cadherin fluorescence intensity was quantified using the hybrid cell count application (Keyence). After the HEV area with positive PNAd signals was manually encircled, the fluorescence intensity of VE-cadherin was automatically quantified in the PNAd-positive area.
Quantification of Intracellular Lipid Uptake
The cells were washed and incubated for 15 min with a 3% Pierce™ formaldehyde solution in PBS at room temperature in the dark. The samples were mounted with Vectashield® Vibrance™ Antifade mounting medium w/ DAPI and stored overnight at room temperature in the dark. Imaging was performed on a Leica TCS SP8 X inverted confocal microscope with 63×/1.40 oil objective magnification. DAPI fluorescence was recorded with a PMT detector at 419 to 484 nm, DiI fluorescence was recorded with a HyD SMD detector at 561 to 606 nm, and the lysotracker fluorescence was recorded with a HyD SMD detector at 657 to 764 nm. For sample excitation, lasers at 405, 553, and 647 nm were used. The illumination settings were kept fixed during the analysis.
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