We used a Leica TCS SP8 confocal laser scanning microscope, white light laser, Leica HCX PL APO 10X/0.4 NA dry objective (2.2 mm working distance) or Leica HC PL APO 40X/1.25 NA oil objective (0.24 mm working distance), and a SuperZ galvometric scanning stage. Using the 10X objective, one half of an immunostained and cleared core was scanned, the sample flipped over, and the other half scanned. 3D scanning was performed in a defined X/Y/Z (1.82/1.82/7.5 μm) volume with 10 frame averaging and bidirectional scanning using 488 nm excitation and 495–530 nm emission filter for DyLight488, 550 nm and 563–579 nm filters for DyLight550, 594 nm and 603–624 nm filter for DyLight594, 633 nm and 648–662 nm filter for DyLight633, 670 nm and 698–721 nm filter for DyLight680, 670 nm and 749–800 nm filter for DyLight755, and 550 nm excitation and 700–722 nm emission filters for LDS 751 nuclear staining.
Hc pl apo 40x 1.25 na oil objective
Manufactured by Leica
The HC PL APO 40X/1.25 NA oil objective is a high-quality microscope objective lens designed for use in various laboratory applications. It features a magnification of 40X and a numerical aperture of 1.25, making it suitable for high-resolution imaging and detailed observation of samples. The lens is optimized for use with oil immersion techniques to enhance image quality and resolution.
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Lab products found in correlation
4 protocols using hc pl apo 40x 1.25 na oil objective
1
Confocal Imaging of Cleared Tissue
2
Multicolor Confocal Imaging of Macrosections
3
Confocal Imaging of Cleared Tissue
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We used a Leica TCS SP8 confocal laser scanning microscope, white light laser, Leica HCX PL APO 10X/0.4 NA dry objective (2.2 mm working distance) or Leica HC PL APO 40X/1.25 NA oil objective (0.24 mm working distance), and a SuperZ galvometric scanning stage. Using the 10X objective, one half of an immunostained and cleared core was scanned, the sample flipped over, and the other half scanned. 3D scanning was performed in a defined X/Y/Z (1.82/1.82/7.5 μm) volume with 10 frame averaging and bidirectional scanning using 488 nm excitation and 495–530 nm emission filter for DyLight488, 550 nm and 563–579 nm filters for DyLight550, 594 nm and 603–624 nm filter for DyLight594, 633 nm and 648–662 nm filter for DyLight633, 670 nm and 698–721 nm filter for DyLight680, 670 nm and 749–800 nm filter for DyLight755, and 550 nm excitation and 700–722 nm emission filters for LDS 751 nuclear staining.
4
Confocal Microscopy Imaging Protocol
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We used a Leica SP5 AOBS II tandem scanner spectral confocal microscope, Leica HCX PL APO 10X/0.4 NA dry objective (2.2 mm working distance) or Leica HC PL APO 40X/1.25 NA oil objective (0.24 mm working distance), and a SuperZ galvometric scanning stage to image immunostained macrosections mounted between coverslips in 80 w/v% D-fructose solutions. 3D scanning was performed in a defined X/Y/Z (1.78/1.78/6.25 μm) volume with 8 frame averaging and bidirectional scanning using 488 nm (Argon) excitation and 495–528 nm emission filter for DyLight488, 514 nm (Argon) and 558–575 nm filters for DyLight550 and Cy3, 561 nm (DPSS) and 585–614 nm filter for DyLight594, 633 nm (HeNe) and 637–655 nm filter for DyLight633, and 633 nm (HeNe) and 713–752 nm filter for DyLight680.
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