According to described previously 21 (link), the closed-chest approach was used to obtain the LV hemodynamics in anesthetized mice using a Millar MPVS-300 system equipped with a Millar SPR-839 catheter. At the end of the experiment, the anesthetized mice were fixed on the operating table, and their neck hair was cut. The ventral side of the neck was cut longitudinally to the right, and the right common carotid artery was isolated on the inside of the sternocleidomastoid muscle. A wedge-shaped incision was then cut, followed by inserting the catheter into the LV through the right carotid artery. The following indicators were used for the continuous monitoring of hemodynamics. Left ventricular end-systolic pressure (LVSP) and left ventricular end-diastolic pressure (LVEDP), and left ventricular systolic and diastolic rate (±dp/dt). The collected data were then analyzed using the Lab Chart Pro software (AD Instruments).
Spr 839 catheter
Manufactured by Millar
The SPR-839 catheter is a medical device designed for various clinical applications. It features a flexible, biocompatible construction and is intended for use by healthcare professionals.
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2 protocols using spr 839 catheter
1
Closed-chest LV Hemodynamics in Mice
2
Noninvasive Cardiac Measurements in Mice
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Blood pressure in conscious mice was measured with a noninvasive tail-cuff system (Kent Scientific Corporation). Surface ECG was recorded in anaesthetized mice (1.5% isoflurane in 95% O2) by using subcutaneous electrodes connected to the Animal Bio amplifier and PowerLab station (AD Instruments). LV hemodynamics were obtained in anaesthetized mice in the closed-chest approach with a Millar MPVS-300 system equipped with a Millar SPR-839 catheter. The mouse was intubated, ventilated, and warmed with a heating pad. The catheter was then inserted into the LV through the right carotid artery. During data collection, ventilation was interrupted to acquire data without lung motion artifacts. Calibration of the SPR-839 catheter and assessment of LV blood volume were performed according to the manufacturer’s instructions. Data were analyzed with LabChart Pro software (AD Instruments).
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