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3 protocols using formamide

1

Nanomedicine for Cancer Therapy

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Sodium hyaluronate (HA, 10 kDa) was purchased from Bloomage Freda Biopharma Co., LTD. Polyethyleneimine (PEI, linear, 25 kDa) was obtained from Polysciences Inc. Qdot™ 800 ITK™ Amino (PEG) Quantum Dots were purchased from Thermo-Fisher Scientific Corp. Inhibitor of miR-27a (anti-miR-27a) and cy3-labeled RNA were purchased from Gene Pharma Co., Shanghai. 1-Ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) and sulforhodamine B were obtained from Sigma-Aldrich. RPMI-1640 medium was obtained from GIBCO. Fetal bovine serum (FBS), penicillin, streptomycin, assay kits for cell cycle and cell apoptosis were from the Beyotime Institute of Biotechnology (Jiangsu, China). Antibodies for western blotting were purchased from Abcam. Assay kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine (CRE) and total triglycerides (TG) were purchased from Nanjing Jiancheng Bioengineering Institute. Formamide and ethyl alcohol were obtained from Beijing Chemical Works. All reagents were commercially available products with analytical grade purity and were used without further purification.
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2

Fabrication of PCDTBT and CN-PPV Devices

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PCDTBT ((C43H47N3S3)nC12H10, Mw = 20 000–100 000)
and CN-PPV ((C36H46N2O4)n) were both provided by Sigma Chemical
Co., Ltd. Mg(NO3)2·6H2O, Al(NO3)3·9H2O, urea, formamide, and toluene
were all of analytical grade and obtained from Beijing Chemical Factory,
China. SiO2 (300 nm)/Si wafer and silver paste were provided
by J&K Co., Ltd.
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3

Synthesis and Characterization of Metal Chalcogenide Nanostructures

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All chemicals were used as received without further purification. Tin (II) bromide (SnBr2, 99 %) and Germanium (IV) chloride (GeCl4, 99.9999 %) were obtained from Sigma-Aldrich. Sodium sulfide nonahydrate (Na2S·9H2O, 98 %) was purchased from Alfa Aesar. Cupric acetate (Cu (OAc) 2, 99 %), sulfur powder (99.99 %), 1-dodecanethiol (98 %), and oleylamine (80–90 %) were obtained from Aladdin. Hexane (analytical reagent), ethanol (analytical reagent), formamide (analytical reagent), and acetone (analytical reagent) were purchased from Beijing chemical works.
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