Cat assay kit

Manufactured by Merck Group

Sourced in United States

The CAT assay kit is a laboratory test kit developed by Merck Group to measure the activity of the enzyme Catalase (CAT) in various biological samples. The kit provides the necessary reagents and protocols to quantify the catalase activity, which is an important antioxidant enzyme found in cells and tissues.

Automatically generated - may contain errors

Lab products found in correlation

Sod assay kit, by Merck Group (2 mentions) Wst 1 reagent, by Merck Group (1 mentions) Gsh standard, by Merck Group (1 mentions) Oxiselect tbars assay kit, by Cell Biolabs (1 mentions) Mda assay kit, by Beyotime (1 mentions) Sod assay kit, by Beyotime (1 mentions) Iron assay kit, by Abcam (1 mentions) Lipid peroxidation mda assay kit, by Abcam (1 mentions) In vitro ros rns assay kit, by Cell Biolabs (1 mentions) Sod activity colorimetric assay kit, by Abcam (1 mentions)

Close spelling variants of this product

Colorimetric CAT assay kit

7 protocols using cat assay kit

Quantifying Algal Oxidative Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

The H₂O₂ content of algal cells were determined by treating the cells with 1 M potassium iodide and the absorbance was read spectrophotometrically at 390 nm. The values were determined from the standard using fresh H₂O₂ solutions [71 (link)]. The MDA content was determined by mixing 1 ml of 0.5% thiobarbituric acid in 20% trichloroacetic acid and 0.5 ml supernatant with vortex mixing. The mixture was kept 15 min in boiling water bath and the cooled mixture was centrifuged at 2790 g for 10 min. The absorbance was read at 450, 532 and 600 nm and the values were used in the following equation to determine the MDA content [72 (link)]:

MDA (μ mol / g fresh weight) = [6.45 * ({OD}_{532} - {OD}_{600})] - [0.56 {OD}_{450}] / fresh weight (g) .

The SOD and CAT activities of hormone-treated lysed cell suspension were determined by WST-1 reagent (Sigma Aldrich, USA) protocol and CAT assay kit (Sigma Aldrich, USA) protocol, respectively.

Sivaramakrishnan R, & Incharoensakdi A. (2020). Plant hormone induced enrichment of Chlorella sp. omega-3 fatty acids. Biotechnology for Biofuels, 13, 7.

+ Open protocol

+ Expand

Oxidative Stress Markers in Kidney Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lipid peroxidation was determined using a method to measure the formation of thiobarbituric acid reactive substances (TBARSs). The level of malondialdehyde (MDA) in kidney tissue was measured spectrophotometrically using an OxiSelect TBARS assay kit (Cell Biolabs, San Diego, CA). MDA values were expressed as nM/mg protein. Total glutathione (GSH) contents of kidney tissue were measured as described previously (34 (link)). A tissue homogenate was prepared, and then samples were added to metaphosphoric acid and allowed to stand for 5 min to precipitate proteins. Phosphate buffer and 5,5′-dithiobis-2-nitro-benzoic acid were added for color development. GSH was determined by measuring absorbance at 415 nm and absolute concentrations were calculated using a GSH standard (Sigma Aldrich, St. Louis, MO). Values of total GSH were expressed as nM/mg protein. Superoxide dismutase (SOD) activity was measured using a SOD assay kit (Fluka). Values of SOD were expressed as U/mg protein. Glutathione peroxidase (GSH-Px) activity was determined using the cellular activity assay kit CGP-1 (Sigma Aldrich). Values of GSH-Px were expressed as U/mg protein. Catalase activity (CAT) was determined by a CAT assay kit (Sigma Aldrich) using the decomposition rate of the substrate H₂O₂ as determined at 240 nm. Total CAT values were expressed as U/mg protein.

Lee B.S., Lee C., Yang S., Ku S.K, & Bae J.S. (2019). Renal protective effects of zingerone in a mouse model of sepsis. BMB Reports, 52(4), 271-276.

+ Open protocol

+ Expand

Antioxidant Evaluation in Retinal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The homogenized retinal tissues or Müller cells were subjected to centrifugation to obtain the supernatants. The levels of SOD (superoxide dismutase), CAT (catalase), and MDA (malondialdehyde) in the supernatants were estimated by a SOD assay kit (#S0101M, Beyotime, China), a CAT assay kit (#CAT100, Sigma-Aldrich, USA), and a MDA assay kit (#S0131M, Beyotime) according to the suppliers' instructions, respectively.

Bian J., Ge W, & Jiang Z. (2024). miR-26a-5p Attenuates Oxidative Stress and Inflammation in Diabetic Retinopathy through the USP14/NF-κB Signaling Pathway. Journal of Ophthalmology, 2024, 1470898.

+ Open protocol

+ Expand

Enzymatic Antioxidant Activities

Check if the same lab product or an alternative is used in the 5 most similar protocols

SOD activity was measured using a SOD assay kit (Fluka). CAT activity was measured using a CAT assay kit (Sigma-Aldrich) based on the decomposition rate of the substrate hydrogen peroxide (H₂O₂), which was measured at 240 nm.

Sim H., Lee W., Choo S., Park E.K., Baek M.C., Lee I.K., Park D.H, & Bae J.S. (2021). Sulforaphane Alleviates Particulate Matter-Induced Oxidative Stress in Human Retinal Pigment Epithelial Cells. Frontiers in Medicine, 8, 685032.

+ Open protocol

+ Expand

Antioxidant Enzyme Activity Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

The analyses were carried out using the commercial assay kit:

–

TAC—OxiSelectTM Total Antioxidant Capacity Assay Kit (Cell BioLabs, Inc., Upper Heyford, UK, no. STA-360);

–

SOD Assay Kit (Sigma Aldrich, Schnelldorf, Germany, no. 1916-1KT-F);

–

CAT Assay Kit (Sigma Aldrich, Schnelldorf, Germany no. CAT100-1KT);

–

GPx—Glutathione peroxidase Assay Kit (Sigma Aldrich, Schnelldorf, Germany, no. MAK437);

–

GSH—EnzyChromTM GSH/GSSG Assay Kit (Bio Assay Systems, Hayward, CA, USA, no. EGTT-100).

All antioxidant enzyme activities were calculated per 1 mg of protein.
The original protocols owned by the companies are available in an electronic version on the manufacturers’ website.

Skowronek P, & Strachecka A. (2023). Cannabidiol (CBD) Supports the Honeybee Worker Organism by Activating the Antioxidant System. Antioxidants, 12(2), 279.

+ Open protocol

+ Expand

Evaluating Oxidative Stress and Inflammation in Kidney Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kidneys from each group were snap-frozen and stored at −80°C until use. Kidney homogenates were prepared according to the protocols of different assays. SOD level and CAT level were assessed with a SOD assay kit and CAT assay kit (Sigma-Aldrich, United States). Kidney tissues of mice in each group were sliced using a frozen micro-slicer to evaluate ROS’s scavenging effect. Frozen kidney tissue slides (about 5 μm thickness) were washed with PBS. One of the slides was stained with 1 mM DCFH-DA for 30 min to detect superoxide formation, and another slide was stained with TNFα- and IL1β antibodies and DAPI to evaluate the development of inflammation in the tissues. Then a cover glass was applied to each slide using Vectashield mounting medium. Finally, a laser confocal microscope was used to observe the results.

Hou X., Shi J., Zhang J., Wang Z., Zhang S., Li R., Jiang W., Huang T., Guo J, & Shang W. (2022). Treatment of Acute Kidney Injury Using a Dual Enzyme Embedded Zeolitic Imidazolate Frameworks Cascade That Catalyzes In Vivo Reactive Oxygen Species Scavenging. Frontiers in Bioengineering and Biotechnology, 9, 800428.

+ Open protocol

+ Expand

Evaluating Glab's Impact on Oxidative Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

To observe the effect of Glab on oxidative stress in NP, activities of superoxide dismutase (SOD) and catalase (CAT), contents of glutathione (GSH), malondialdehyde (MDA), and iron, as well as intracellular reactive oxygen species (ROS) were respectively measured by colorimetric SOD activity assay kit (#ab65354; Abcam), CAT assay kit (#CAT100; Sigma-Aldrich), fluorometric GSH assay kit (#ab65322; Abcam), lipid peroxidation (MDA) assay kit (#ab118970; Abcam), iron Assay kit (#ab83366; Abcam), and in vitro ROS/RNS assay kit (#STA-347; Cell Biolabs) following protocols provided by the manufacturers.

Tan H., Chen J., Li Y., Li Y., Zhong Y., Li G., Liu L, & Li Y. (2022). Glabridin, a bioactive component of licorice, ameliorates diabetic nephropathy by regulating ferroptosis and the VEGF/Akt/ERK pathways. Molecular Medicine, 28, 58.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!