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Tenovin 1

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Sourced in Canada, United States

Tenovin-1 is a small molecule inhibitor that targets the sirtuin family of enzymes. It is commonly used in laboratory research applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) 27 ohc, by Santa Cruz Biotechnology (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Fulvestrant, by Cayman Chemical (1 mentions) β estradiol, by Merck Group (1 mentions) Nutlin 3, by Cayman Chemical (1 mentions) Sodium phenylbutyrate, by Merck Group (1 mentions) Panobinostat, by Cayman Chemical (1 mentions) Sirtinol, by Cayman Chemical (1 mentions) Dulbecco s modified eagle medium dmem f12, by Thermo Fisher Scientific (1 mentions) Senescence detection kit, by Abcam (1 mentions) Anti cox 2, by Santa Cruz Biotechnology (1 mentions) Tween 20 and ecltm western blotting detection reagent, by Cytiva (1 mentions) Anti cdk2 and anti cdk4, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Merck Group (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Anti gfap, by Merck Group (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)

3 protocols using tenovin 1

1

Exploring p53 and Estrogen Signaling

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27-OHC was purchased from Santa Cruz Biotechnologies (Santa Cruz, CA), the p53 activator, tenovin-1 from Tocris Bioscience (Ellisville, MO), Nutlin-3 and Fulvestrant from Cayman Chemicals (Ann Arbor, MI), the reporter constructs encoding p53 response elements conjugated to the firefly luciferase gene from SA Biosciences (Frederick, MD), and β-estradiol from Sigma-Aldrich (St. Louis, MO). All cell culture reagents, with the exception of fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA) were from Invitrogen (Carlsbad, CA). Human MCF7 and MDA-MB 231 cell lines were purchased from ATCC (Manassas, VA).
Raza S., Ohm J.E., Dhasarathy A., Schommer J., Roche C., Hammer K.D, & Ghribi O. (2015). The cholesterol metabolite 27-hydroxycholesterol regulates p53 activity and increases cell proliferation via MDM2 in breast cancer cells. Molecular and cellular biochemistry, 410(1-2), 187-195.
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2

Resuspension and Dilution of Common Histone Deacetylase Inhibitors

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Drugs for this study were purchased as follows: tenovin-1 was purchased from Tocris Bioscience (catalog no. 336510), sirtinol from Cayman Chemical (catalog no. 10523), sodium phenylbutyrate from Sigma (catalog no. P21005), panobinostat from Cayman Chemical (catalog no. 13280), selisistat/EX527 from Cayman Chemical (catalog no. 10009798), and AGK2 from Cayman Chemical (catalog no. 13145). Drugs were resuspended in dimethyl sulfoxide (DMSO), except for NaPB, which was resuspended in water. Drugs were diluted in culture media for use.
Hackett B.A., Dittmar M., Segrist E., Pittenger N., To J., Griesman T., Gordesky-Gold B., Schultz D.C, & Cherry S. (2019). Sirtuin Inhibitors Are Broadly Antiviral against Arboviruses. mBio, 10(4), e01446-19.
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3

Tenovin-1 Modulates Cellular Signaling

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The following materials were used in this study: Dulbecco’s modified Eagle medium (DMEM)/F12, Penicillin-Streptomycin (P/S), 0.25% trypsin-EDTA from Gibco BRL (Grand Island, NY, USA); 10% Fetal Bovine Serum (FBS) from Gibco BRL, and dimethyl sulfoxide from Invitrogen (Carlsbad, CA, USA); Tween® 20 and ECLTM Western blotting detection reagent from Amersham Life Science (Arlington Heights, IL, USA); tenovin-1 from Tocris bioscience (Minneapolis, MN, USA); anti-β-actin from Sigma (St. Louis, MO, USA); anti-iNOS from Abcam (Cambridgeshire, UK); anti-COX2 from Santa Cruz Biotechnology (CA, USA); anti-phospho-H3 and anti-histone H3 from Cell Signaling Technology (MA, USA); anti-CDK2 and anti-CDK4 from Santa Cruz Biotechnology; anti-GFAP from EMD Millipore (MA, USA); senescence detection kit from Abcam.
Bang M., Ryu O., Kim D.G., Mabunga D.F., Cho K.S., Kim Y., Han S.H., Kwon K.J, & Shin C.Y. (2018). Tenovin-1 Induces Senescence and Decreases Wound-Healing Activity in Cultured Rat Primary Astrocytes. Biomolecules & Therapeutics, 27(3), 283-289.
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