Anti mouse pe

Manufactured by Jackson ImmunoResearch

Anti-mouse PE is a fluorescent dye-conjugated antibody used for the detection and quantification of mouse-derived proteins or cells in various immunoassays and flow cytometry applications.

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2 protocols using anti mouse pe

Comprehensive Antibody Panel for Immunoanalysis

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The following primary antibodies were used for flow cytometry: anti-MICA (clone 159227; R&D Systems), anti-MICB (clone 236511, R&D Systems), anti-ULBP1 (clone 170818; R&D Systems), anti-ULBP2/5/6 (clone 165903; R&D Systems) and anti-ULBP3 (clone 166514, R&D Systems).
The following primary antibodies were used for immunofluorescence: anti-PDI (ab3672, Abcam), anti-FLAG tag (Clone L5, Biolegend) and anti-MICA (clone 159227, R&D Systems).
The following primary antibodies were used for western blotting: anti-MICA (Clone EPR6568, Abcam), anti-FLAG tag (Clone L5, Biolegend), anti-GAPDH (clone 6C5, Santa Cruz) and anti-vinculin (clone EPR8185, Abcam).
The following secondary antibodies were used: anti-mouse AlexaFluor 647, anti-mouse PE, anti-mouse biotin, anti-rabbit biotin, anti-rat biotin, anti-rabbit Cy3, anti-rat 488, streptavidin-AlexaFluor 647, streptavidin-horseradish peroxidase (HRP), anti-mouse-HRP, anti-rat-HRP and anti-rabbit-HRP, all purchased from Jackson Laboratories.

Seidel E., Dassa L., Schuler C., Oiknine-Djian E., Wolf D.G., Le-Trilling V.T, & Mandelboim O. (2021). The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing. PLoS Pathogens, 17(5), e1008807.

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LRP6 Cell Surface Expression

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HEK293T cell were transfected for 48 hr prior to splitting into 60 cm plates with 400,000 cells/plate and allowed to attach overnight. Cells were disassociated with 0.5 mM EDTA in DPBS and washed with FACS Buffer (2% FBS in DPBS). Cells were stained in FACS buffer for 1 hour at on ice using 5 μg/mL LRP6 antibody (R&D) or Isotype Control (R&D) and secondary staining with 1:100 anti-mouse PE (Jackson Immunoresearch, West Grove, PA) for 45 mins. After staining, cells were fixed with 2% paraformaldehyde in FACS buffer and analyzed by the UNC-Flow Cytometry Core.

Agajanian M.J., Walker M.P., Axtman A.D., Ruela-de-Sousa R.R., Serafin D.S., Rabinowitz A.D., Graham D.M., Ryan M.B., Tamir T., Nakamichi Y., Gammons M.V., Bennett J.M., Couñago R.M., Drewry D.H., Elkins J.M., Gileadi C., Gileadi O., Godoi P.H., Kapadia N., Müller S., Santiago A.S., Sorrell F.J., Wells C.I., Fedorov O., Willson T.M., Zuercher W.J, & Major M.B. (2019). WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop. Cell Reports, 26(1), 79-93.e8.

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