Peroxidase conjugated goat anti rabbit igg

The protein samples were analyzed by SDS polyacrylamide gel electrophoresis [25 (link)]. SDS-PAGE gels were prepared using a 5% stacking gel and a 15% separation gel (MDBio, Taiwan, China). The proteins were visualized by staining with Coomassie Blue R-250 (Sangon Biotech, Qingdao, China).
Western blot analysis was performed using Rb c-phycocyanin (Maded by Boster Biological Technology co.ltd, Qingdao, China) and peroxidase-conjugated goat anti-rabbit IgG (Sangon Biotech, Qingdao, China) as primary and secondary antibodies, and the dilution factors were 1:1000 (primary antibodies) and 1:2000 (secondary antibodies). The expression of phycocyanin was judged by observing whether there is a hybridization strip in the nitrocellulose membrane.

SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) was prepared using polyacrylamide gel premix (MDBio, China), with 5% stacking gel and 15% separating gel. After electrophoresis, the gel was stained with Coomassie Brilliant Blue R250 solution or transferred to polyvinylidene fluoride membrane for Western blot detection.
Native-PAGE (native polyacrylamide gel electrophoresis) was prepared using polyacrylamide gel premix (MDBio, China), with a stacking gel of 6% and a separating gel of 8%. After electrophoresis, it was stained with Coomassie Brilliant Blue R250 solution.
In western blot detection, to detect the protein expression, the primary antibody was histidine-tag antibody (Sangon Biotech, China), secondary antibody peroxidase-conjugated goat anti-mouse IgG (Sangon Biotech, China); and to specifically detect the target phycobiliprotein, the primary antibody was phycocyanin and allophycocyanin specific antibody (Sino Biological Inc., China), and the secondary antibody was peroxidase-conjugated goat anti-rabbit IgG (Sangon Biotech, China). After hybridization, DAB solution (MDBio, China) was used for color development.