Mouse tnfα high sensitivity elisa

Manufactured by Thermo Fisher Scientific

The Mouse TNFα High Sensitivity ELISA is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of mouse tumor necrosis factor alpha (TNFα) levels in cell culture supernatants, serum, and plasma samples.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

TNFα mouse high sensitivity ELISA kit TNFα ELISA high sensitivity High sensitivity ELISA Mouse TNFα ELISA TNFα ELISA ELISAs

2 protocols using mouse tnfα high sensitivity elisa

Quantifying TNFα in Bone Marrow

Check if the same lab product or an alternative is used in the 5 most similar protocols

TNFα in bone marrow supernatants was quantitated by Mouse TNFα High Sensitivity ELISA (Thermo Fisher). Further information is available in Supplemental Experimental Procedures.

Patterson A.M., Liu L., Sampson C.H., Plett P.A., Li H., Singh P., Mohammad K.S., Hoggatt J., Capitano M.L., Orschell C.M, & Pelus L.M. (2020). A Single Radioprotective Dose of Prostaglandin E2 Blocks Irradiation-Induced Apoptotic Signaling and Early Cycling of Hematopoietic Stem Cells. Stem Cell Reports, 15(2), 358-373.

+ Open protocol

+ Expand

Quantifying Protein and Cytokine Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein samples were thawed on ice and diluted 1:50 (for BCA protein analysis, Micro BCA Protein Analysis Kit, ThermoFisher Scientific) or 1:8 (for ELISA, Mouse TNF-α High Sensitivity ELISA, ThermoFisher Scientific) in ice-cold ddH20. Total protein and TNF-α quantification were performed in duplicates following manufacturers’ protocols. TNF-α levels were calculated as pg/mg total protein, then normalized to non-pregnant control group.

Zupan B., Liu B., Taki F., Toth J.G, & Toth M. (2017). Maternal brain TNF programs innate fear in the offspring. Current biology : CB, 27(24), 3859-3863.e3.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!