Waters 2998 uv vis diode array detector

Manufactured by Waters Corporation

The Waters 2998 UV-Vis Diode Array Detector is a laboratory instrument used for the detection and analysis of chemical compounds. It employs a diode array technology to provide full spectral information across a wide wavelength range. The detector is designed to be used in conjunction with liquid chromatography systems for the identification and quantification of analytes.

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Lab products found in correlation

Waters 2424 els detector, by Waters Corporation (4 mentions) 3100 mass detector, by Waters Corporation (3 mentions) Xbridge beh c18 column, by Waters Corporation (2 mentions) Avance ultrashield 400 mhz spectrometer, by Bruker (1 mentions) Tco peg4 nhs, by Vector Laboratories (1 mentions) Waters 3100 mass detector, by Waters Corporation (1 mentions) Xterra ms c18 column, by Waters Corporation (1 mentions) Ascend 400 mhz spectrometer, by Bruker (1 mentions) 2475 multi wavelength fluorescence detector, by Waters Corporation (1 mentions) Microflex maldi tof ms, by Bruker (1 mentions) Dual fl spectrophotometer, by Horiba (1 mentions)

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Waters 2998 (39 citations) UV detector (22 citations) Diode array detector (12 citations) 2998 diode array detector (11 citations) Waters 2998 diode-array detector (5 citations) Waters 2998 UV detector (4 citations) UV–VIS detector (4 citations)

4 protocols using waters 2998 uv vis diode array detector

Synthesis and Characterization of Clickable Reagents

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All reagents were obtained from commercial sources and used without further purification. TCO-PEG₃-Amine, TCO-PEG₄-NHS, Methyltetrazine-PEG₄-NHS, and tetrazine-fluorophores (AF488, Cy3, AF594) were purchased from Click Chemistry Tools, LLC. Flash column chromatography was performed using Sorbtech purity flash cartridges or Biotage SNAP Bio C18 columns for reversed phase chromatography. NMR spectra were recorded on a Bruker Avance UltraShield 400 MHz spectrometer. Chemical shifts are reported in parts per million (δ) and referenced to the residual solvent. Reactions were monitored via liquid chromatography-mass spectrometry (LC-MS) on a Waters instrument equipped with a Waters 2424 ELS Detector, Waters 2998 UV-Vis Diode array Detector, and a Waters 3100 Mass Detector. UV-Vis analysis of antibodies was performed on a NanoDrop 1000 spectrophotometer.

Marquard A.N., Carlson J.C, & Weissleder R. (2020). Expanding the scope of antibody rebridging with new pyridazinedione-TCO constructs. Bioconjugate chemistry, 31(6), 1616-1623.

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NMR Spectroscopy and HPLC-MS Analysis

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NMR spectra were recorded on a Bruker Avance UltraShield 400 MHz spectrometer. ¹H NMR chemical shifts were reported in ppm relative to SiMe₄ (δ = 0) and were referenced internally concerning residual protons (δ = 4.79 for D₂O). Peak assignments, calculated chemical shifts, and peak integrals were based on reference solvent peaks. 2D Rotating Frame Overhauser Enhancement Spectroscopy (2D‐ROESY) experiments were performed to assess the interactions between dipolarly coupled hydrogens, and integrals were normalized to the reference hydrogen (H¹) of the sbCD. All experiments were performed in D₂O (0.5 mL) at a fixed sCD concentration (26 mM) with 10% (CD₃)₂SO. High‐performance liquid chromatography‐mass spectrometry analysis (HPLCMS) was performed on a Waters instrument equipped with a Waters 2424 ELS Detector, Waters 2998 UV–Vis Diode array Detector, and a Waters 3100 Mass Detector. Separations employed an HPLC‐grade water/acetonitrile (0.1% formic acid) solvent gradient with XTerra MS C18 Column, 125 Å, 5 µm, 4.6 × 50 mm column; Waters XBridge BEH C18 Column, 130 Å, 3.5 µm, 4.6 × 50 mm.

Enbergs N., Halabi E.A., Goubet A., Schleyer K., Fredrich I.R., Kohler R.H., Garris C.S., Pittet M.J, & Weissleder R. (2024). Pharmacological Polarization of Tumor‐Associated Macrophages Toward a CXCL9 Antitumor Phenotype. Advanced Science, 11(15), 2309026.

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NMR and HPLC-MS Characterization of Cyclodextrin Complexes

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NMR spectra were recorded on a Bruker Avance UltraShield 400 MHz spectrometer. ¹H NMR chemical shifts are reported in ppm relative to SiMe₄ (δ = 0) and were referenced internally concerning residual protons (δ = 4.79 for D₂O). Peak assignments, calculated chemical shifts and peak integrals are based on reference solvent peaks. Two-dimensional Rotating Frame Overhauser Enhancement Spectroscopy (2D-ROESY) experiments were performed to assess the interactions between dipolarly coupled hydrogens, and integrals were normalized to the reference hydrogen (H¹) of the s-β-CD. All experiments were performed in D₂O (0.7 mL) at a fixed s-β-CD concentration (26 mM) with 10% (CD₃)₂SO. High-performance liquid chromatography-mass spectrometry analysis (HPLC-MS, LCMS) was performed on a Waters instrument equipped with a Waters 2424 ELS Detector, Waters 2998 UV-Vis Diode array Detector, and a Waters 3100 Mass Detector. Separations employed an HPLC-grade water/acetonitrile (0.1% formic acid) solvent gradient with XTerra MS C18 Column, 125Å, 5 μm, 4.6 mm X 50 mm column; Waters XBridge BEH C18 Column, 130Å, 3.5 μm, 4.6 mm X 50 mm.

Harrod R, & Lovett P.S. (1995). Peptide inhibitors of peptidyltransferase alter the conformation of domains IV and V of large subunit rRNA: a model for nascent peptide control of translation.. Proceedings of the National Academy of Sciences of the United States of America, 92(19), 8650-8654.

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Comprehensive Analytical Characterization

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¹H and ¹³C nuclear magnetic resonance spectra were recorded on a Bruker Ascend 400 MHz spectrometer. MALDI data was acquired on a Bruker microflex MALDI-TOF MS. Silica Gel 60 (40–63 μm) was used for flash column purification. High performance liquid chromatography-mass spectrometry analysis (HPLC-MS) was performed with a Waters instrument equipped with a Waters 2424 ELS Detector, Waters 2998 UV–vis Diode array Detector, Waters 2475 Multiwavelength Fluorescence Detector, and a Waters 3100 Mass Detector. Separations employed Waters XTerra RP C18 5 μm or Waters XSelect CSH Fluoro-Phenyl 2.5 μm column, with a water:acetonitrile solvent gradient (0.1% formic acid). Fluorescence measurements were conducted with a QuantaMaster 400 fluorimeter (PTI, New Jersey, USA), and UV–vis absorption spectra on a HORIBA Dual-FL spectrophotometer.

Meimetis L.G., Boros E., Carlson J.C., Ran C., Caravan P, & Weissleder R. (2015). Bioorthogonal Fluorophore Linked DFO–Technology Enabling Facile Chelator Quantification and Multimodal Imaging of Antibodies. Bioconjugate chemistry, 27(1), 257-263.

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