Autospec premier

Manufactured by Waters Corporation

Sourced in United States

The Autospec Premier is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a modular design, allowing users to configure the system to meet their specific requirements. The Autospec Premier provides precise and reproducible separations, ensuring reliable and consistent results.

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Lab products found in correlation

Gf254 plates, by Qingdao Haiyang Chemical (3 mentions) Voyager de pro, by Thermo Fisher Scientific (1 mentions) Av 600 spectrometer, by Bruker (1 mentions) Agilent 6210 esi tof, by Agilent Technologies (1 mentions) Ecz 400r, by JEOL (1 mentions) Ecs 400 spectrometer, by JEOL (1 mentions) Nicolet 380 ft ir instrument, by Thermo Fisher Scientific (1 mentions) G1311c 1260 quat pump vl, by Agilent Technologies (1 mentions) Packed c18, by YMC (1 mentions) G1315d 1260 dad vl detector, by Agilent Technologies (1 mentions) Avance 500 nmr spectrometer, by Bruker (1 mentions) Sephadex lh 20, by Merck Group (1 mentions) Avance 2, by Bruker (1 mentions) Micromass lct tof, by Waters Corporation (1 mentions) Ecx 400, by JEOL (1 mentions) Avance 700, by Bruker (1 mentions)

Close spelling variants of this product

AutoSpec Premier P776 spectrometer (18 citations) AutoSpec Premier P776 (14 citations) Autospec Premier P776 mass spectrometer (13 citations) Micromass Autospec Premier (2 citations)

5 protocols using autospec premier

Synthesis and Characterization of 10,10'-Dibromo-9,9'-bianthryl

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Unless otherwise
stated, all manipulations
of air- and/or moisture-sensitive compounds were carried out in oven-dried
glassware under an atmosphere of N₂. All solvents and reagents
were purchased from Alfa Aesar, Spectrum Chemicals, Acros Organics,
TCI America, and Sigma-Aldrich and used as received unless otherwise
noted. Organic solvents were dried by passing through a column of
alumina and were degassed by vigorous bubbling of N₂ through
the solvent for 20 min. Flash column chromatography was performed
on SiliCycle silica gel (particle size 40–63 μm). Thin-layer
chromatography was carried out using SiliCycle silica gel 60 Å
F-254 precoated plates (0.25 mm thick) and visualized by UV absorption.
All ¹H and ¹³C NMR spectra were recorded on
a Bruker AV-600 spectrometer and are referenced to residual solvent
peaks (CDCl₃, ¹H NMR = 7.26 ppm, ¹³C NMR = 77.16 ppm; CD₂Cl₂, ¹H NMR
= 5.32 ppm, ¹³C NMR = 53.84 ppm). EI mass spectrometry
was performed on an AutoSpec Premier (Waters) system in positive ionization
mode. MALDI mass spectrometry was performed on a Voyager-DE PRO (Applied
Biosystems Voyager System 6322) instrument in positive mode using
a matrix of dithranol. 10,10′-Dibromo-9,9′-bianthryl
was synthesized following a reported procedure.^{74 (link)}

Jacobse P.H., Daugherty M.C., Čerņevičs K., Wang Z., McCurdy R.D., Yazyev O.V., Fischer F.R, & Crommie M.F. (2023). Five-Membered Rings Create Off-Zero Modes in Nanographene. ACS Nano, 17(24), 24901-24909.

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Characterization of Organoboron Compounds

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Dried solvents and argon as protection gas were used. ¹¹B, ¹³C, ¹⁹F, ²⁹Si, and ⁷⁷Se NMR spectra were recorded with a JEOL ECZ 400R or with a JEOL ECS 400 spectrometer (128.25, 100.51, 376.13, 79, and 76.24 MHz, respectively). Chemical shifts δ are reported in ppm relative to BF₃⋅OEt₂ (¹¹B), Me₄Si (¹³C, ²⁹Si), CFCl₃ (¹⁹F), and Me₂Se (⁷⁷Se).
For ESI‐TOF mass spectra, the samples were measured from CH₃CN, CH₃CN/CH₂Cl₂, CH₃OH, or CH₃CN/CH₃OH solutions with an Agilent 6210 ESI‐TOF, Agilent Technologies, Santa Clara, CA, USA. The solvent flow rate was adjusted to 4 μL min⁻¹, spray voltage set to 4 kV, drying gas flow rate was set to 15 psi (1 bar; ESI‐TOF=electrospray ionization–time of flight). Non‐ionic compounds were analyzed with a HR‐EI‐MS (Autospec Premier, Waters Co., Milford, MA, USA) using 80 eV electron energy.

Poleschner H, & Seppelt K. (2020). Attempts to Synthesize a Thiirane, Selenirane, and Thiirene by Dealkylation of Chalcogeniranium and Thiirenium Salts. Chemistry (Weinheim an Der Bergstrasse, Germany), 27(2), 649-659.

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Analytical Techniques for Natural Products

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Optical rotations were measured on an Anton Paar MCP 5100 polarimeter. IR spectra were obtained using a Nicolet 380 FT-IR instrument (Thermo, USA) using KBr pellets. HRESIMS were determined by a Waters Autospec Premier (Waters, USA) mass spectrometer. The NMR spectra were recorded on Bruker Avance 500 NMR spectrometers (Bruker, Germany) with TMS as an internal standard. The high performance liquid chromatography (HPLC) was performed with an analytic reversed-phased column (YMC–packed C₁₈, 250 mm × 10 mm, 5 µm) (YMC, Japan) using a G1311C 1260 Quat Pump VL and detected with a G1315D 1260 DAD VL detector (190–500 nm) (Agilent Technologies 1260 infinity, USA). For column chromatography, silica gel (60–80, 200–300 mesh, Qingdao Haiyang Chemical Co., Ltd, China), ODS gel (20–45 µm, Fuji Silysia Chemical Co., Ltd, USA), and Sephadex LH-20 (Merck, Germany) were used. TLC analysis was performed on precoated silica gel GF254 plates (Qingdao Haiyang Chemical Co., Ltd, China), and spots were visualized by spraying with 5% H₂SO₄ in EtOH followed by heating. Thermo fisher scientific plate reader was used for enzyme inhibition assays.

Yang L., Yang Y.L., Dong W.H., Li W., Wang P., Cao X., Yuan J.Z., Chen H.Q., Mei W.L, & Dai H.F. (2019). Sesquiterpenoids and 2-(2-phenylethyl)chromones respectively acting as α-glucosidase and tyrosinase inhibitors from agarwood of an Aquilaria plant. Journal of Enzyme Inhibition and Medicinal Chemistry, 34(1), 853-862.

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Organometallic Compound Synthesis Procedures

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Most manipulations of organometallic compounds were carried out using standard Schlenk techniques under an atmosphere of dry argon. CH₂Cl₂, hexane, toluene, and chloroform were dried by distillation over CaH₂, pyridine over KOH. Melting points were determined on a Kofler apparatus of the Boetius type. ¹H and ¹³C NMR spectra were recorded on a Bruker Avance II spectrometer (400 and 100 MHz, respectively). Spectra are referenced relative to the chemical shift (δ) of TMS. Mass spectra were obtained with Micromass LCT TOF and AutoSpec Premier (Waters) spectrometers. Infrared spectra were recorded on a FT-IR spectrometer as KBr pellets or as solid samples using the ATR technique. Flash chromatography (FC) was performed on silica gel 230–400 mesh. Yields refer to chromatographically purified products unless otherwise stated. Catalysts 3 and 5 were commercially available. Substrates for testing catalysts in RCM reactions were prepared by allylation of commercial diethyl malonate with allyl bromide and/or 3-chloro-2-methylpropene according to Hensle [24 (link)]. Their purity was estimated by ¹H NMR spectroscopy and found to be at least 95 %. Other chemicals are commercially available and used as received. N-Mesityl-N’-(2-nitrophenyl)ethylenediamine (9) was prepared according to literature [14 (link)].

Malinowska M., Kozlowska M., Hryniewicka A., Witkowski S, & Morzycki J.W. (2016). New indenylidene-type metathesis catalysts bearing unsymmetrical N-heterocyclic ligands with mesityl and nitrobenzyl substituents. Monatshefte Fur Chemie, 147, 1091-1100.

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NMR and Mass Spectra Analysis

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1 H and 13 C NMR spectra were acquired on a JEOL ECX 400 (400 MHz), JEOL ECP 500 (500 MHz) and a Bruker Avance 700 (700 MHZ) in CDCl3 as a solvent. The chemical shifts were reported relative to CDCl3 (δ = 1 H: 7.26 ppm, 13 C: 77.16 ppm). The multiplicities of the signals are described using the following abbreviations: s = singlet, d = doublet, t = triplet, q = quartet, p = quintuplet, br = broad and combinations thereof.
The spectra were evaluated with the software MestRec.
Mass spectra were obtained on a ESI-FTICR-MS: Ionspec QFT-7 (Agilent/Varian), or a HR-EI-MS: Autospec Premier (Waters).

Schwan J., Kleoff M., Hartmayer B., Heretsch P, & Christmann M. (2018). Synthesis of Quinolinone Alkaloids via Aryne Insertions into Unsymmetric Imides in Flow. Organic letters, 20(23).

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