Whole blood samples were collected prior to challenge (baseline) and on days 2, 4, 6, and 8 post-challenge for clinical hematology, and processed to serum for clinical chemistry analyses. Complete blood cell counts and hematology values were measured using an Advia® 120 Hematology Analyzer (Siemens, Deerfield, IL). Clinical chemistry analyses were performed using an Advia® 1200 Chemistry System (Siemens).
Advia chemistry system 1200
Manufactured by Siemens
Sourced in United Kingdom
The ADVIA Chemistry System 1200 is an automated, high-throughput clinical chemistry analyzer designed for routine and specialized laboratory testing. It offers a comprehensive menu of assays and delivers reliable results to support clinical decision-making. The system's core function is to perform a wide range of in vitro diagnostic tests on various biological samples, such as serum, plasma, and urine, using advanced spectrophotometric and potentiometric technologies.
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5 protocols using advia chemistry system 1200
1
Longitudinal Blood Analysis Post-Challenge
2
Serum Biomarker Analysis Protocol
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Serum levels of amylase, lipase, and a full panel of clinical chemistry parameters were measured at the time points previously described using Advia 1200 Chemistry System from Siemens according to manufacturer's instructions.
3
Lactate Quantification in Heart Tissue
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Lactate was measured in heart tissue lysates using ADVIA Chemistry Lactate Enzymatic Assay and ADVIA Chemistry System 1200 (Siemens). Lactate is oxidized by lactate oxidase to pyruvate and hydrogen peroxide, and it was measured by the formation of dye from hydrogen peroxide and a chromogen in the presence of a peroxidase according to manufacturer instructions. Absorbance was measured at 545/694 nm.
4
Measurement of Lactate and LDH in Heart Tissue
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Lactate was measured in heart tissue lysates using ADVIA Chemistry Lactate Enzymatic Assay and ADVIA Chemistry System 1200 (Siemens, Frimley, UK). Lactate is oxidised by lactate oxidase to pyruvate and hydrogen peroxide and it was measured by the formation of dye from hydrogen peroxide and a chromogen in the presence of a peroxidase. Absorbance was measured at 545/694 nm. LDH was measured using ADVIA Chemistry System 2400 (Siemens, Frimley, UK). Lactate dehydrogenase catalyses the conversion of lactate to pyruvate in the presence of NAD. The enzymatic activity of lactate dehydrogenase is proportional to the rate of production of NADH. The amount of NADH produced is determined by increase in absorbance at 340/410 nm.
5
Enzymatic Lactate Quantification in Heart
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Lactate was measured in heart tissue lysates using ADVIA Chemistry Lactate Enzymatic Assay and ADVIA Chemistry System 1200 (Siemens, Frimley, UK). Lactate is oxidized by lactate oxidase to pyruvate and hydrogen peroxide and it was measured by the formation of dye from hydrogen peroxide and a chromogen in the presence of a peroxidase. Absorbance was measured at 545/694 nm.
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