L cysteine

Manufactured by Merck Group

Sourced in United States, Germany, United Kingdom, Italy, France, Canada, Poland, Sao Tome and Principe, Norway, Japan, Sweden, China, Israel, Macao, Switzerland, Spain, Austria, Brazil

L-cysteine is an amino acid that serves as a key component in the manufacturing of various laboratory reagents and equipment. It functions as a building block for proteins and plays a crucial role in the formulation of buffers, cell culture media, and other essential laboratory solutions.

Automatically generated - may contain errors

Lab products found in correlation

737 protocols using l cysteine

In Vitro Evaluation of Synergistic VSC Production

Check if the same lab product or an alternative is used in the 5 most similar protocols

An in vitro VSCs assay was carried out to test the hypothesis that S. moorei and F. nucleatum cultures in combination could produce higher amounts of VSCs, compared to the individual cultures. This assay was performed as described previously [7 (link)]. Briefly, S. moorei and F. nucleatum were cultured in broth until reaching the logarithmic phase (0.5 < optical density < 1.0) and were then centrifuged at 2876 g for 5 min. The pellets were washed and the bacterial inoculum was prepared in phosphate buffer saline (PBS; pH 7.7) at an optical density of 0.1 at 660 nm.
The following suspensions were kept in 20 mL headspace vials and incubated at 37°C for 1 h: 1) S. moorei—100 μL of S. moorei inoculum + 885 μL of PBS + 15 μL of 33 mM L-cysteine (Sigma, Poole, UK); 2) F. nucleatum—100 μL of F. nucleatum inoculum + 885 μL of PBS + 15 μL of 33 mM L-cysteine; 3) S. moorei + F. nucleatum—100 μL of S. moorei inoculum + 100 μL of F. nucleatum inoculum + 785 μL of PBS + 15 μL of 33 mM L-cysteine.
After incubation, the reaction was stopped by the addition of 500 μL of 3 M phosphoric acid during 10 min. A 1 mL volume of the headspace gas was sampled and the H₂S production was measured using the Oral Chroma™ system, as described in Section 2.1.3. Under these experimental conditions, CH₃SH and (CH₃)₂S were not detectable. The experiment was carried out using nine replicates.

Nani B.D., de Lima P.O., Marcondes F.K., Groppo F.C., Rolim G.S., de Moraes A.B., Cogo-Müller K, & Franz-Montan M. (2017). Changes in salivary microbiota increase volatile sulfur compounds production in healthy male subjects with academic-related chronic stress. PLoS ONE, 12(3), e0173686.

+ Open protocol

+ Expand

Intracerebroventricular l-Cysteine and AOAA Administration After SAH

Check if the same lab product or an alternative is used in the 5 most similar protocols

l-Cysteine (Sigma-Aldrich) was dissolved in vehicle (PBS) at a working concentration of 100 mM as determined by our previous research (15 (link)), and 30 µL of the l-cysteine solution was intracerebroventricularly administered 30 min after SAH. AOAA (Sigma-Aldrich) was dissolved in vehicle (PBS), and a 5 mg/kg dose was intraperitoneally administered with l-cysteine.

Li T., Wang L., Hu Q., Liu S., Bai X., Xie Y., Zhang T., Bo S., Gao X., Wu S., Li G, & Wang Z. (2017). Neuroprotective Roles of l-Cysteine in Attenuating Early Brain Injury and Improving Synaptic Density via the CBS/H2S Pathway Following Subarachnoid Hemorrhage in Rats. Frontiers in Neurology, 8, 176.

+ Open protocol

+ Expand

Crosslinking of 293T cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

1 × 10⁶ 293T cells were exposed to disuccinimidyl suberate (DSS; 1 mM, 0.3 mM, 0.1 mM), 1,8-bismaleimido-diethyleneglycol (BM(PEG)2; 1 mM, 0.3 mM, 0.1 mM) or N-γ-maleimidobutyryl-oxysuccinimide ester (GMBS; 1 mM, 0.3 mM, 0.1 mM) crosslinking for 5 min at R.T. in PBS or permeabilised with 100 μL of reaction buffer including 125 mM NaCl, 50 mM Tris pH 7.0 (for BM(PEG)2) or 50 mM PIPES pH7.0 (for DSS or GMBS), 5 mM MgCl₂ and 0.1% Triton X-100 at 37°C for 10 min before crosslinking. After quenching DSS with 20 mM Tris pH 7.0, BM(PEG)2 with 20 mM L-cysteine (Sigma-Aldrich) or GMBS with 20 mM Tris pH 7.0 and 20 mM L-cysteine, cells were processed for western blotting. 0.3 mM cross-linker was used for routine experiments while 1 mM was used for SAF-A IPs (Figures 4C and 4E).
For Figure 4B right, reaction buffer was supplemented with 5 mM ATP or ATPγS. For Figure 4D, after pre-treatment with 3 μg.ml^-1 PureLink RNaseA (ThermoFisher) in reaction buffer at 37°C for 10 min, 293T cell lysates were incubated in the presence or absence of 30 μg.ml^-1 total RNA from 293T cells and 5 units.ml^-1 Apyrase (NEB) at 37°C for 10 min, following by crosslinking. After RNaseA treatment reaction buffer was supplemented with RNasin Plus RNase Inhibitor (Promega) 1000 units.ml^-1, 1 mM DTT and 5 mM CaCl₂.

Nozawa R.S., Boteva L., Soares D.C., Naughton C., Dun A.R., Buckle A., Ramsahoye B., Bruton P.C., Saleeb R.S., Arnedo M., Hill B., Duncan R.R., Maciver S.K, & Gilbert N. (2017). SAF-A Regulates Interphase Chromosome Structure through Oligomerization with Chromatin-Associated RNAs. Cell, 169(7), 1214-1227.e18.

+ Open protocol

+ Expand

Culturing Clostridium difficile Strain 630

Check if the same lab product or an alternative is used in the 5 most similar protocols

Clostridium difficile strain 630 was purchased from ATCC (BAA-1382-FZ). The growth medium contained 38 g/L Brain-Heart Infusion (BHI, BD), 5 g/L yeast extract (Sigma), and 0.1% L-cysteine (Sigma). C. difficile cells were grown at 37 °C without shaking in an anaerobic chamber (Coy Laboratories). BHI contains 15 g/L peptone, 10 g/L peptone No. 3, 2 g/L dextrose, 5 g/L NaCl, 2.5 g/L Na₂HPO₄. For the preparation of agar plates, 38 g BHI, 5 g yeast extract and 15 g agar (Sigma) were mixed in 1 L DI water, autoclaved at 121 °C for 20 min, cooled to ~50 °C, and supplemented with 10 mL of filter-sterilized 10% (w/v) L-cysteine. Around 30 mL of the mixture was poured into each sterile 100 × 15 mm petri dish (Krackeler). The plates were kept in the anaerobic chamber for at least one day prior to use.

Wu X., Paskaleva E.E., Mehta K.K., Dordick J.S, & Kane R.S. (2016). Wall Teichoic Acids Are Involved in the Medium-Induced Loss of Function of the Autolysin CD11 against Clostridium difficile. Scientific Reports, 6, 35616.

+ Open protocol

+ Expand

Magnetic Anisotropy in (Ga,Mn)As Thin Films

Check if the same lab product or an alternative is used in the 5 most similar protocols

The AHPA-L in the experiments was obtained from RS Synthesis, LLC. L-cysteine, MHA, and ODT were acquired from Sigma-Aldrich, Inc. Molecules, except L-cysteine, were dissolved in pure ethanol at 1 mM concentration. L-cysteine was dissolved in deionized water at a concentration of 2.5 mM. The AHPA-L solution was kept at -18 ^oC for storage whereas L-cysteine, MHA and ODT solution were stored in ambient conditions.
The (Ga,Mn)As with perpendicular magnetic anisotropy was grown by low-temperature molecular-beam epitaxy (LT-MBE). 500 nm thick (In,Ga)As buffer layer was first grown at 450 °C on semi-insulating (001) GaAs substrates. (Ga,Mn)As films of 40 nm thickness with perpendicular magnetic anisotropy were later grown at substrate temperature of 270 °C. The (Ga,Mn)As thin film has a Curie temperature of 80 K and coercive field of 174 Oe.

Adhikari Y., Liu T., Wang H., Hua Z., Liu H., Lochner E., Schlottmann P., Yan B., Zhao J, & Xiong P. (2023). Interplay of structural chirality, electron spin and topological orbital in chiral molecular spin valves. Nature Communications, 14, 5163.

+ Open protocol

+ Expand

Isolation of Diverse Swine Gut Microbiota

Check if the same lab product or an alternative is used in the 5 most similar protocols

The broad spectra of commensal bacteria were isolated from colon and fecal samples of pigs/piglets bred in Czech farms (n = 25, samples taken at slaughterhouses) and wild boars (n = 25, samples collected during hunting). Samples were collected in tubes containing 5 g/L tryptone, 5 g/L nutrient broth No. 2, 2.5 g/L yeast extract (all Oxoid, Basingstoke, UK), 0.5 g/L L-cysteine, 1 mL/L tween 80 (both Sigma-Aldrich, St. Louis, MO, USA), and 30% glycerol (V.W.R., Radnor, PA, USA). After the sample collection and transfer to the place of analysis, log 10 serial dilutions of samples were plated on different media cultivated under various cultivation conditions (Table 1).
Bacterial isolates were obtained based on different cultivation characteristics of colonies from all used media. The oxygen, temperature, and substrate requirements were secured for the following sub-cultivation. Anaerobes were cultured in Wilkins–Chalgren Anaerobe broth supplemented with soya pepton (5 g/L; both Oxoid), L-cysteine (0.5 g/L), and tween 80 (1 mL/L, both Sigma-Aldrich), while aerobes in tryptone Soya broth (Oxoid), both for 24 h. Then, bacterial isolates were stored as stock cultures at −80 °C in 30% glycerol.

Horvathova K., Modrackova N., Splichal I., Splichalova A., Amin A., Ingribelli E., Killer J., Doskocil I., Pechar R., Kodesova T, & Vlkova E. (2023). Defined Pig Microbiota with a Potential Protective Effect against Infection with Salmonella Typhimurium. Microorganisms, 11(4), 1007.

+ Open protocol

+ Expand

Formulated Growth Media for Anaerobic Bacteria

Check if the same lab product or an alternative is used in the 5 most similar protocols

Supplemented Brain–Heart Infusion medium: Brain–Heart Infusion base (BD, Franklin Lakes, NJ, USA) was supplemented with yeast extract (5 g l⁻¹), vitamin (2 μ l⁻¹), hemin (5 mg l⁻¹), cysteine (0.5 g l⁻¹) and 5% each of newborn calf serum, horse serum, and sheep serum.
Supplemented LB medium: LB base in powder form (Sigma, St Louis, MO, USA) was combined with l-cysteine (Sigma), added KH₂PO₄ (6 g l⁻¹), (NH₄)₂SO₄ (6 g l⁻¹), NaCl (12 g l⁻¹), MgSO₄·7H₂O (2.5 g l⁻¹), CaCl₂·2H₂O (1.6 g l⁻¹), l-cysteine (0.25 g l⁻¹) (see detailed formulation in the Supplementary Materials) and deionized water, which was autoclaved at 121 °C for 20 min. After cooling, vitamin K1 (9.84 mg l⁻¹) and filter sterilized (0.22 μm pore size) solutions of hemin (0.005 g l⁻¹), lactose (0.05 g l⁻¹) and Tween-20 (0.01 g l⁻¹) were added.
All media was equilibrated overnight in the anaerobic chamber before inoculation with ASF members.

Biggs M.B., Medlock G.L., Moutinho T.J., Lees H.J., Swann J.R., Kolling G.L, & Papin J.A. (2016). Systems-level metabolism of the altered Schaedler flora, a complete gut microbiota. The ISME Journal, 11(2), 426-438.

+ Open protocol

+ Expand

Partitioning of MeHg-Cysteine Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Partitioning of MeHg complexed with L-cysteine was evaluated for the
ag+AC passive samplers. Isotherm solutions were spiked with a MeHgCl standard
(Brooks Rand Laboratories) diluted to five concentrations ranging from 30 to
3000 ng/L. All experimental isotherms were conducted in 60-mL polyethylene
terephthalate glycol (PETG) copolymer bottles. L-cysteine (Sigma-Aldrich, 98%)
was added to each experimental bottle to maintain a 10⁵:1 molar ratio
of L-cysteine to MeHg, and buffered with the addition of 1mM MOPS
(3-morpholinopropane-1-sulfonic acid). Solutions were incubated overnight in the
dark on a shaker table at 60 rpm at 4 °C prior to sampler addition to
ensure a stable MeHg distribution. Samplers were added to experimental bottles
in triplicate at each concentration level, and were allowed to equilibrate for
14 d in the dark on a shaker table at 60 rpm at 4 °C. After 14 d, ag+AC
samplers were carefully removed with forceps, then stored frozen. A portion of
the overlying water was acidified to 0.5% v/v with trace metal grade HCl to
preserve MeHg, then stored refrigerated at 4 °C.

Washburn S.J., Damond J., Sanders J.P., Gilmour C.C, & Ghosh U. (2022). Uptake Mechanisms of a Novel, Activated Carbon-Based Equilibrium Passive Sampler for Estimating Porewater Methylmercury. Environmental toxicology and chemistry, 41(9), 2052-2064.

+ Open protocol

+ Expand

Culturing Francisella novicida Strain U112

Check if the same lab product or an alternative is used in the 5 most similar protocols

Francisella tularensis subsp. novicida strain U112 (hereafter F. novicida) and the derivative strains were grown at 37 °C with aeration in brain heart infusion (BHI) medium supplemented with 0.2% L-cysteine (Sigma) and appropriate antibiotics. Antibiotic concentrations used were 100 μg ml⁻¹ ampicillin (AppliChem) or 15 μg ml⁻¹ kanamycin (AppliChem). A detailed strain list can be found in Supplementary Table 1. For infection with F. novicida, BHI medium was inoculated with bacteria from BHI agar plate (supplemented with 0.2% L-cysteine (Sigma) and appropriate antibiotics) and were grown overnight at 37 °C with aeration.

Brodmann M., Dreier R.F., Broz P, & Basler M. (2017). Francisella requires dynamic type VI secretion system and ClpB to deliver effectors for phagosomal escape. Nature Communications, 8, 15853.

+ Open protocol

+ Expand

Xanthan Gum Solution Preparation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aqueous solutions were prepared in either Phosphate Buffered Saline (PBS, Gibco without calcium and magnesium) or Remel^™ Butterfield’s Phosphate Buffer, Thermo Fisher Scientific (Waltham, MA). A 0.5% (weight/volume in buffer) xanthan gum (XG) solution (from Xanthomonas campestris), Sigma-Aldrich (St. Louis, MO), was prepared one day prior to conducting an experiment. The mixture was shaken at 35°C typically for 3 h to initiate the mixing process and then sterilized by autoclaving. After sterilization, the mixture was homogeneous and could be stored until use at room temperature. Additives such as L-cysteine (L-cysteine, ≥ 98.5% BioUltra), and sodium thioglycolate were obtained from Sigma-Aldrich (St. Louis, MO). Peptone buffered water (PBW, 5 g/mL) was purchased from Neogen (Lansing, MI). Sanicult Medium was purchased from Thermo Fisher Scientific (Waltham, MA).

Hutchison J.R., Brooks S.M., Kennedy Z.C., Pope T.R., Deatherage Kaiser B.L., Victry K.D., Warner C.L., Oxford K.L., Omberg K.M, & Warner M.G. (2019). Polysaccharide-based liquid storage and transport media for non-refrigerated preservation of bacterial pathogens. PLoS ONE, 14(9), e0221831.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!