One normal human breast epithelial cell line (MCF-10A) and four BC cell lines (MDA-MB-231, MDA-MB-468, MCF7 and MDA-MB-415) were obtained from the Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). These cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco-BRL, Grand Island, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA). All the cells were maintained at 37°C in a humidified incubator with 5% CO2.
Mda mb 231
Manufactured by Shanghai Cell Bank
Sourced in China
MDA-MB-231 is a cell line derived from a human breast adenocarcinoma. It is commonly used in cancer research as a model for triple-negative breast cancer. The core function of MDA-MB-231 is to provide a well-characterized in vitro system for studying various aspects of breast cancer biology and evaluating potential therapeutic interventions.
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Lab products found in correlation
Mcf 7, by Shanghai Cell Bank (39 mentions)
Mcf 10a, by Shanghai Cell Bank (21 mentions)
Sk br 3, by Shanghai Cell Bank (17 mentions)
Mda mb 468, by Shanghai Cell Bank (14 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (11 mentions)
Mda mb 453, by Shanghai Cell Bank (10 mentions)
Bt 549, by Shanghai Cell Bank (9 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (9 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions)
Penicillin, by Merck Group (6 mentions)
Streptomycin, by Merck Group (6 mentions)
Fetal bovine serum (fbs), by Merck Group (6 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (6 mentions)
Trypsin, by Thermo Fisher Scientific (4 mentions)
Bt474, by Shanghai Cell Bank (4 mentions)
Mcf 7, by Thermo Fisher Scientific (4 mentions)
Hs578t, by Shanghai Cell Bank (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
62 protocols using mda mb 231
1
Breast Cancer Cell Line Culture
2
Xenograft Model of Breast Cancer
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Forty BALB/cnu-nu female nude mice aged 5-6 weeks and weighing 16-20 g (SLACK, Shanghai, China) were housed in the specific pathogen-free (SPF) environment. Human breast cancer cell line MDA-MB-231 (Shanghai Cell Bank, Chinese Academy of Sciences) was maintained at 37°C in L-15 medium containing 10% FBS. When the cell confluence reached 100%, cells in the logarithmic growth phase were harvested, digested, and centrifuged, and then cells were resuspended in normal saline at a density of 2 × 107/ml. Each mouse was subcutaneously inoculated with 0.1 ml of cell suspension at the right axillary.
3
Culturing Breast Cell Lines
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Normal breast epithelial cell MCF-10A and breast cancer cell lines SK-BR-3, MCF-7, MDA-MB-231, and MDA-MB-468 were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. For the experiment, the cells were first thawed and then re-suspended in DMEM medium containing +1% penicillin-streptomycin solution +10% fetal bovine serum. The cells were transferred to culture dishes and observed at 37°C and 5%CO2.
4
Cell Culture Protocol for Breast Cancer Lines
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MCF‐7, SK‐BR‐3, MDA‐MB‐231, and MDA‐MB‐453 cell lines were obtained from Shanghai Cell Bank. All cells were cultured in RPMI‐1640 medium (Invitrogen) containing 10% FCS (Invitrogen), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Sigma‐Aldrich). The cells were grown in sterile culture dishes at 37°C in a 5% CO2 atmosphere and passaged every 2 days, using 0.25% trypsin (Invitrogen) for cell detachment. All cell lines were authenticated using short tandem repeat DNA profiling.
5
Diverse Cancer Cell Line Cultures
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Human chronic myelogenous leukemia K562 cells, human promyelocytic leukemia HL-60 cells, human acute T lymphocytic leukemia Jurkat cells, human acute myeloid leukemia cell line Kasumi-1, human cervical carcinoma HeLa cells, human cervical squamous carcinoma SiHa cells, human cervical cancer cell lines CaSki, human hepatocellular carcinoma BEL-7402, human lung adenocarcinoma cell line A549, human breast cancer MDA-MB-231, human prostate cancer PC-3, mouse hepatoma cell line H22, and murine breast carcinoma cell line 4T1 were obtained from Shanghai Cell Bank, Chinese Academy of Science. The human non-small cell lung cancer cell line NCI-H1975 was provided by the American Type Culture Collection. A549 cell line was cultured in Ham’s F12K medium (F12K) with 10% fetal bovine serum and 1% penicillin/streptomycin. The K562 cell line was cultured in Iscove’s Modified Dulbecco’s medium (IMDM) with 10% fetal bovine serum and 1% penicillin/streptomycin. HeLa cell line was cultured in modified Eagle’s medium (MEM) with 10% fetal bovine serum and 1% penicillin/streptomycin, and others were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium with 10% fetal bovine serum and 1% penicillin/streptomycin. All the cells were cultured at 37 °C with 5% CO2 in a humidified incubator.
6
Culturing Human Breast Cancer Cells
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Human breast cancer cell lines MDA-MB-231 and MDA-MB-435S were obtained from the Shanghai Cell Bank of the Chinese Academy of Science, Shanghai, China. Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Corning, U.S.A.) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. All cells were cultured in a humidified incubator (Thermo Fisher Scientific, Waltham, MA, U.S.A.) with 5% CO2 at 37°C.
7
Culturing Breast Cell Lines for Research
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Normal breast epithelial cells MCF10A and breast cancer cell lines MDA-MMB-436, HS578T, SKBR3, MDA-MB-231, and MCF-7 were purchased from the Shanghai Cell Bank of the Chinese Academy of Science. MCF10A cells were maintained in Dulbecco's Modified Eagle Medium (DMEM)/F12 medium supplemented with 5% horse serum, epidermal growth factor (20 ng/mL), insulin (10 µg/mL), hydrocortisone (0.5 mg/mL), cholera toxin (100 ng/mL), and penicillin (100 U/mL) and streptomycin (100 µg/mL) (Thermo Fisher, Waltham, USA). MDA-MMB-436, HS578T, MDA-MB-231, and MCF-7 were cultured in Roswell Park Memorial Institute 1640 medium containing 10% fetal bovine serum (FBS, Life Technology, Pleasanton, USA), 2mM L-glutamine, 20 mM HEPES, 1 mM sodium pyruvate, 100 U/mL penicillin, and 100 μg/mL streptomycin medium supplemented with 2 mM glutamine and 15% FBS. HS578T cells were cultured in DMEM medium supplemented with 10% FBS, 0.01 mg/mL bovine insulin and 100 U/mL penicillin, and 100 μg/mL streptomycin (Thermo Fisher). Cells were cultured in a 5% saturated CO2 atmosphere at 37°C.
8
Establishment and Maintenance of Cancer Cell Lines
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The human breast cancer cell lines MDA-MB-231, MCF-7 and T-47D, human hepatocellular carcinoma (HCC) cell lines HepG2 and SMMC7721, human nasopharyngeal carcinoma cell lines HNE1 and CNE-2Z, human gastric cancer cell line SGC7901, human endometrial cancer cell line ISK, and human non-small cell lung cancer cell line A549 were bought from Shanghai Cell Bank (Shanghai, China). The human colon carcinoma SW480 was obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were obtained, frozen and cultured in our laboratory. The cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) or Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco), supplemented with 10 % fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were grown in an atmosphere containing 5%CO2 at 37 °C.
9
Breast Cancer Cell Culture Protocols
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Breast cancer cell lines MDA-MB-231, MCF-7 and 4T1 were purchased from the Shanghai Cell Bank located in the Chinese Academy of Science (Shanghai, China). MDA-MB-231 cells were cultured in DMEM high glucose complete medium, containing 10% foetal bovine serum and 1% penicillin-streptomycin. MCF-7 and 4T1 cells were cultured using RPMI 1640 complete medium containing 10% foetal bovine serum and 1% penicillin-streptomycin. Cells were cultured under conditions of 5% CO2 at 37 °C.
10
Culturing Human Breast Cancer Cells
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Human breast cancer cell lines Michigan Cancer Foundation (MCF)-7 and MDA-MB-231 were purchased from the Chinese Academy of Sciences Shanghai Cell Bank. The cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 complete medium (Gibco, Carlsbad, California, USA) at 37 °C in an incubator containing 5% CO2. The complete medium comprised 10% fetal bovine serum (FBS), penicillin (100 U/ml) and streptomycin (100 μg/ml).
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