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355 protocols using acetone

1

Synthesis of Fluorescent Polyurethane Polymer

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Isophorone diisocyanate (IPDI) and polytetrahydrofuran glycol 2000 (PTHF2000) were bought from Aladdin (Shanghai) Co., Ltd. Polycarbonate diol 2000 (PCDL2000) was procured from Nanjing Chemical Material Corporation Co., Ltd. The 1-((2-hydroxyethyl)amino)-4-(methylamino)-10-anthracenedione (NH–blue–OH, as shown in Scheme 1) in a raw powder form was acquired from FILO Color & Chemicals (Wuxi) Co., Ltd. Dibutyltin dilaurate (DBTDL) and acetone were all obtained from Sinopharm Chemical Reagent Co., Ltd. acetone was dried with CaH2 and always kept with 4A molecular sieves before utilization.
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2

Gelatin Extraction and Characterization

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Type A gelatin from pigskin was purchased from China National Pharmaceutical Group Corporation (Beijing, China) and used after dialysis. The isoelectric point (pI, 8.5) of the dialyzed gelatin after complete deionization was determined by fluorescence spectroscopy. Sodium dodecyl sulfate (SDS) was purchased from Alfa Aesar and recrystallized from ethanol before use. Ally glycidyl ether (AGE) and chloroplatinic acid hexahydrate (H2Pt6Cl6•6H2O) were obtained from Alfa Aesar. Hexamethylcyclotrisiloxane (D3, >95%), n–butyllithium (C4H9Li, >99%), and chlorodimethylsilane (C2H7ClSi, >99%) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Benzene, deionized water (conductivity = 2.06 µS cm−1, dielectric constant ε = 80.40), methanol (ε = 32.70), ethanol (ε = 24.50), isopropanol alcohol (ε = 17.90), acetone (ε = 20.70), tetrahydrofuran (THF, ε = 7.58), and 1,4-dioxane (ε = 2.25) solvents (China National Pharmaceutical Group Corporation) were all of analytical reagent (AR) grade and strictly dehydrated before use.
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3

Phytochemical Profiling of Carrots

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We used carrots from Beinong market in Beijing, China. Abscisic acid (chromatographic grade, ≥98%) and ethephon (chromatographic grade, ≥90%) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Methanol, ethanol, and acetonitrile were purchased from Merk Ltd. (Darmstadt, Germany). BHT (chromatographic grade, ≥99%) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Acetone was purchased from the China National Pharmaceutical Group Corporation (Beijing, China). Methyl tertbutyl ether was purchased from CNW Technologies GmbH (Dusseldorf, Germany). NaCl and KOH were purchased from Shanghai Rhawn Chemical Technology Co., Ltd. (Shanghai, China). All of the standards (chromatographic grade, ≥95%) were purchased from Olchemim Ltd. (Olomouc, Czech Republic) and Sigma (Saint Louis, MO, USA). Formic acid was obtained from Sigma and BOC (New York, NY, USA).
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Quantitative Detection of CRP and PCT

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Goat anti-mouse IgG, mouse monoclonal CRP antibody (CRP135 mAb), mouse monoclonal PCT antibody (14C12 mAb), its paired mouse monoclonal calcitonin antibody (14A2 mAb), CRP-free serum, and PCT-free serum were obtained from Hytest. Analytically pure reagents: cadmium oxide (CdO), selenium powder (Se), powdered sulfur (S), oleic acid (OA), zinc oxide powder (ZnO), octadecene (ODE), octadecyl amine (ODA), trioctylphosphine oxide (TOPO), sodium azide were obtained from Sigma-Aldrich; mercaptoacetic acid (MAA), trichloromethane, normal hexane, methanol, acetone, heptane, glycine, sodium tetraborate, and sucrose were from China National Pharmaceutical Group. Standard human CRP and standard human PCT, bovine serum albumin (BSA), and phosphate-buffered saline (PBS) were from Sigma-Aldrich. Guarantee reagents: N-hydroxysuccinimide (NHS) and carbodiimide (EDC) were from China National Pharmaceutical Group. Nitrocellulose membrane and glass cellulose membrane were from Millipore; the whole blood separator was from Shanghai JieYi Biotechnology; and the stickiness baseplate was from Shanghai Liangxin Science and Technology Ltd. Ultrapure water (Milli-Q) is used in the detections.
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5

Bacterial Cellulose Hydrolysis and Analysis

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Bacterial cellulose was provided by Hainan Yida Co., Ltd. (Guangdong, China). Cellulase from Aspergillus niger (product #C0057, 10 000 U/g, slightly brown powder) was purchased from Okyo Chemical Industry (TCI). The following chemicals were purchased from Sinopharm Chemical Reagent Co., Ltd: dimethylacetamide (DMAc, ≥ 99%), lithium chloride (LiCl, ≥97%), sulfuric acid (≥98%), Avicel citric acid (≥99.5%), sodium citrate dihydrate (meets USP testing specifications), 3,5-dinitrosalicylic acid (DNS, ≥98%), ethyl alcohol (EtOH, ≥99%), acetone (≥
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Synthesis of Cesium Lead Bromide Perovskites

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Cesium bromide (CsBr, 99.5% metals basis), lead(ii) bromide (PbBr2, AR, 99.0%), oleylamine (OM, 80–90%) and dimethyl sulfoxide (DMSO) were purchased from Aladdin®. Ethyl acetate (EA), n-hexane (n-H), oleic acid (OA, AR), absolute ethanol and acetone were purchased from Sinopharm Chemical Reagent. All reagents were used as received without any further purification.
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7

Quantitative Analysis of PCBs

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The compounds in Table 1 were provided in a standard solution from o2si smart solutions (Charleston, SC, USA) containing 18 PCB congeners (indicator PCBs: 28, 52, 101, 118, 138, 153, and 180; dioxin-like PCBs: 77, 81, 105, 114, 118, 123, 126, 156, 157, 167, 169, and 189) at 10 mg/L each in n-hexane. MWCNTs-COOH (length, 10–30 μm; diameter, 10–20 nm) were purchased from Nanjing XFNANO Materials Tech Co., Ltd. (Nanjing, China). Primary secondary amine (PSA; particle size, 40–60 μm), graphitized carbon black (GCB; particle size, 40–120 μm), and octadecylsilane (C18; particle size, 40–60 μm) were from Tianjin Bonna-Agela Technologies Co., Ltd. (Tianjin, China). n-Hexane was of HPLC grade and purchased from Oceanpak Alexative Chemical., Ltd (Goteborg, Sweden). All other chemical reagents like acetonitrile, acetone, toluene, anhydrous Na2SO4, and anhydrous MgSO4 in this research were of analytical grade or better and purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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8

Fabrication of PVDF-HFP/PI Composite Membranes

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Polyvinylidene fluoride hexafluoropropylene (PVDF-HFP, Mw = 400,000) was purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Polyimide (PI) resin was supplied by the Hangzhou Plastic UNITA Special Technology Co., Ltd. (Hangzhou, China). Dimethylformamide (DMF) and acetone were obtained from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All chemical reagents were used as received without further purification.
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9

Synthesis and Characterization of Zr-based MOF for PCA Detection

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All reagents used in this study were of analytical grade unless otherwise stated. 2-aminoterephthalic acid (ATA) (99%) and Zirconium chloride (ZrCl 4 ) (98%) were gained from Aladdin Reagent Co., Ltd. (Shanghai, China). Dimethylformamide (DMF) (99%) , tetrahydrofuran (THF), mesitylene (97%), acetonitrile (CAN)and 1,4-dioxane were gained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 1,3,5-Tris (4-aminophenyl) benzene (TAPB) (97%) and 2,5-Divinylterephthalaldehyde (DVA) were obtained from Shanghai Macklin Biochemical Technology Co., Ltd., (Shanghai, China). Methanol, acetone, and glacial acetic acid (HAC) were achieved from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). 4-chloro-2-methylphenoxy acetic acid (MCPA), 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4Dichlorprop (2,4-DP), 2,4dichlorophenoxybutyric acid (2,4-DB), and 2-(2,4,5-trichlorophenoxy) propanoic acid (2,4,5-TP), were purchased from ANPEL Laboratory Technology Inc. (Shanghai, China). For the standard solution of 100 μg⋅L -1 , five kinds of PCAs were dissolved in a 100 mL volumetric flask with Methanol and stored in 4 • C. A series of working aqueous solutions were obtained by diluting the standard solution with pure water.
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10

Synthesis of Metal-Organic Frameworks

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All solvents and chemicals used in this research were commercial products. Ethanol, N, N−dimethylformamide (DMF), mEthanol, chloroform, ethyl acetate, acetone, acetonitrile, zinc chloride (ZnCl2), p−benzenedicarboylic acid (p−H2BDC), propylene carbonate (PC), Ethanol, lithium perchlorate (LiClO4) and potassium carbonate were purchased from Sinopharm Chemical reagent Co. Ltd. (Shanghai, China), 2,5−Dibromoanisole, 4−pyridineboronic acid, (beta−4)−platinum and tris(dibenzylideneacetone)dipalladium(0), 4−Bromomethylbenzoic acid, and 1,4−Dioxane were bought from Energy Chemical (Shanghai, China).
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