Mab453

Manufactured by R&D Systems

Sourced in United States

MAB453 is a mouse monoclonal antibody that recognizes human PDGF receptor alpha. It can be used for the detection of PDGF receptor alpha in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

MAB453-500 (2 citations)

8 protocols using mab453

Murine Intracranial Aneurysm Model

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murine intracranial aneurysms were created in female 8-12 week-old C57BL/6 mice (Charles River Laboratories, Wilmington, MA) using a method described previously.^{10 (link)} For the antibody blockade, 100 μg/mL anti-CXCL1 antibody (MAB453, R&D Systems) or 100 μg/mL IgG2A control antibody were injected retro-orbitally every two days.

Nowicki K.W., Hosaka K., He Y., McFetridge P.S., Scott E.W, & Hoh B.L. (2014). A novel high-throughput in vitro model for identifying hemodynamic-induced inflammatory mediators of cerebral aneurysm formation. Hypertension, 64(6), 1306-1313.

+ Open protocol

+ Expand

Dorsal Air Pouch Inflammation Model

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dorsal air pouches were prepared by injection of 2.5 mL of air on day 0 and day 3 in CD-1 mice, as previously described.25 (link) On day 6, mice received 0.25 mL of one of the following treatments diluted in 0.5% carboxymethyl cellulose (CMC, Sigma-Aldrich): (1) vehicle, CMC alone; (2) IL-17 (1 µg); (3) nIL-17 (1 µg); (4) IL-17 (1 µg) plus MAB421 or Ab-IPL-IL-17 (10 µg); (5) IL-17 (1 µg) plus anti-JE (10 µg, MAB479, R&D System) and (6) IL-17 (1 µg) plus anti-KC (10 µg, MAB453, R&D System). Mice were sacrificed after 24 hours, and lavage fluids were recovered, and centrifuged at 220 g for 10 min at 4°C. Cell pellets and inflammatory exudates were banked for subsequent analysis of inflammatory cyto-chemokines. The route, timing and frequency of administration as well as the selected dosages of tested compounds were selected according to updated literature.12 16 (link) Cell number was determined by TC20 automated cell counter (Bio-Rad) using Bio-Rad’s TC20 automated cell counter uses disposable slides, TC20 trypan blue dye (0.4% trypan blue dye w/v in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution, Sigma-Aldrich) and a CCD camera to count cells based on the analyses of capture images.25 (link)

Saviano A., Manosour A.A., Raucci F., Merlino F., Marigliano N., Schettino A., Wahid M., Begum J., Filer A., Manning J.E., Casillo G.M., Piccolo M., Ferraro M.G., Marzano S., Russomanno P., Bellavita R., Irace C., Amato J., Alfaifi M., Rimmer P., Iqbal T., Pieretti S., Vellecco V., Caso F., Costa L., Giacomelli R., Scarpa R., Cirino G., Bucci M., McGettrick H.M., Grieco P., Iqbal A.J, & Maione F. (2023). New biologic (Ab-IPL-IL-17) for IL-17-mediated diseases: identification of the bioactive sequence (nIL-17) for IL-17A/F function. Annals of the Rheumatic Diseases, 82(11), 1415-1428.

+ Open protocol

+ Expand

Bone Marrow Labeling by Microinjection

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bone marrow tagging by microinjection were performed as previously described^{29 (link)}. Microinjection of the tibia marrow was performed sequentially under 0.3% pentobarbital anesthesia, using a 1 ml syringe mounted with a 5 mm-outer-diameter veterinary needle. For each procedure aseptic technique was used. For the tibia marrow injection, we injected at the chosen injection site of tibia plateau just below the knee and the bone wall was perforated with a veterinary needle. The red cell tracker or CXCL1/2 antibodies (Clone # 48415, R&D Systems, MAB453, 0.2 μg/ml or 5 ng/mice once; Clone # 40605, R&D Systems, MAB452, 2 μg/ml or 40 ng/mice once) was injected in the marrow cavity over 30 s. Animals were sacrificed 12 h after injection.

Yu Y., Wang R.R., Miao N.J., Tang J.J., Zhang Y.W., Lu X.R., Yan P.Y., Wang J, & Jia X.M. (2022). PD-L1 negatively regulates antifungal immunity by inhibiting neutrophil release from bone marrow. Nature Communications, 13, 6857.

+ Open protocol

+ Expand

Cytokine Profiling of Respiratory Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Culture supernatants and BAL samples were assayed for IL-1β, MIP-1α, TNFα, IL-6, IL-10, IL-18, IL-17A and CXCL1 by ELISA. Antibody pairs for the IL-1β (MAB401 and BAF401; clone 30311 and polyclonal; R&D Systems; 8 and 4 μg ml⁻¹, respectively) and CXCL1 (mouse: MAB453 and BAF453; clone 48415 and polyclonal; R&D Systems; 8 and 0.4 μg ml⁻¹, respectively; human: MAB275, clone 20326, 4 μg ml⁻¹; BAF275, polyclonal, 40 ng ml⁻¹) and CCL3/ MIP-1α (Quantikine ELISA kit, Product #MMA00) ELISAs were from R&D Systems. Antibody pairs for IL-1α (capture: product #14-7011-85, clone ALF-161; detection: product #13-7111-85, polyclonal), TNFα (capture: product #14-7325-85, clone 1F3F3D4; detection: product #13-7326-85, clones MP6-XT22 and MP6-XT3), IL-6 (capture: product #14-7061-85, clone MP5-20F3; detection: product #13-7062-85, clone MP5-32C11), IL-10 (capture: product #14-7101-85, clone JES5-16E3; detection: product #13-7102-85, clone JES5-2A5) and IL-17A (capture: product #14-7175-85, clone eBio 17CK15A5; detection: product #13-7177-85, clone eBio17B7) were from eBiosciences and used at a 1:250 dilution. Antibody pairs for IL-18 were from MBL International (capture: product #D047-3, clone 74, 1:1,000; detection: product #D048-6, clone 93-10C, 1:2,000).

Ulland T.K., Jain N., Hornick E.E., Elliott E.I., Clay G.M., Sadler J.J., Mills K.A., Janowski A.M., Volk A.P., Wang K., Legge K.L., Gakhar L., Bourdi M., Ferguson P.J., Wilson M.E., Cassel S.L, & Sutterwala F.S. (2016). Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment. Nature Communications, 7, 13180.

+ Open protocol

+ Expand

Modulating intestinal barrier via CXCL1

Check if the same lab product or an alternative is used in the 5 most similar protocols

One day after the completion of the DSS treatment, mice received an intraperitoneal injection of 100 µg of either a rat anti-mouse CXCL1 (R and D Systems Cat# MAB453, RRID: AB_2087696) or a rat IgG2A isotype control (R and D Systems Cat# MAB006, RRID: AB_357349). Intestinal barrier function was assessed 3 weeks after the treatments using Ussing chambers as described above.

Grubišić V., Bali V., Fried D.E., Eltzschig H.K., Robson S.C., Mazei-Robison M.S, & Gulbransen B.D. (2022). Enteric glial adenosine 2B receptor signaling mediates persistent epithelial barrier dysfunction following acute DSS colitis. Mucosal Immunology, 15(5), 964-976.

+ Open protocol

+ Expand

Staphylococcus aureus Skin Abscess Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were anaesthetized intraperitoneally (i.p.) with pentobarbital sodium (50 mg/kg) before shaving the dorsal area. The mice were then subcutaneously (s.c.) injected with the S. aureus strain USA300-TCH1516 (1 × 10⁷ CFU) in 50 μL of sterile phosphate buffered saline (PBS). The abscess area was measured using a Vernier caliper and photographed with a millimetre ruler as a reference. Mice were euthanized, and the lesional skin was collected by 8 mm punch biopsies at day 2 post-infection. A portion of the skin was homogenized mechanically in cold PBS (at a ratio of 5 mL per gram tissue), and the total bacterial burden was determined by plating out serial dilutions on tryptic soy broth (TSB) agar plates. In other experiments, 100 μg of SB225002 (Selleck, Houston, TX, USA) was administered i.p. 1 h prior to infection and 23 h post-infection. The control mice received equal amounts of vehicle. 5 μg of MAB453 (R&D, Emeryville, CA, USA) was administered i.p. 1 h prior to infection and 23 h post-infection. The control mice received equal amounts of isotype IgG.

Liu Z.Z., Yang Y.J., Zhou F.H., Ma K., Lin X.Q., Yan S.Q., Gao Y, & Chen W. (2021). GSDMD contributes to host defence against Staphylococcus aureus skin infection by suppressing the Cxcl1–Cxcr2 axis. Veterinary Research, 52, 71.

+ Open protocol

+ Expand

Conditioned Media Effects on Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

For conditioned media preparation, EO771 cells were plated on 6 well plates at a density of 5×10⁴ cells/well and cultured for 96 hours. Conditioned media were collected, centrifuged for 5min at 1500 rpm and added for 48h in 3T3 fibroblasts that had been cultured for 16 hours in serum free media on 12-well plates at a density of 5×10⁵ cells/well. Experiments were performed in triplicates, and similar results were obtained.
For Ccl3 and Cx1 neutralization, antibodies were added in the conditioned media after their collection at concentrations 20 μg/ml for mouse monoclonal Ccl3 (MAB450; R&D Systems) and 1ug/ml for mouse monoclonal Cxcl1 (MAB453; R&D Systems).

Farmaki E., Kaza V., Papavassiliou A.G., Chatzistamou I, & Kiaris H. (2016). Induction of the MCP Chemokine Cluster Cascade in the Periphery by Cancer Cell-Derived CCL3. Cancer letters, 389, 49-58.

+ Open protocol

+ Expand

Depletion of CXCL1 or CCL2 from Conditioned Medium

Check if the same lab product or an alternative is used in the 5 most similar protocols

Conditioned medium was depleted of CXCL1 or CCL2 using antibodies previously loaded onto protein A/G Plus-Agarose beads (Santa Cruz Biotechnology, Dallas TX) at a ratio of 10 μL beads to 1μg antibody. Beads were washed before and after antibody loading. Antibodies were purified anti-CXCL1 (R and D systems MAB453, Minneapolis MN) used at 2 μg/mL conditioned medium, anti-CCL2 (R and D systems AF-479) at 5 μg/mL, Rat IgG2a isotype control (R and D systems MAB006) at 2 μg/mL, or goat IgG (R and D systems AB-108) at 5 μg/mL. Incubation with conditioned medium was on a rocking platform overnight at 4°C, after which the beads were pelleted and the depleted conditioned medium was collected and used for downstream applications.

Dill R, & Walker A.M. (2016). Role of Prolactin in Promotion of Immune Cell Migration into the Mammary Gland. Journal of mammary gland biology and neoplasia, 22(1), 13-26.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!