The EV size and shape analysis was conducted by cryo-EM. Samples (4 µL) were adhered to QUANTIFOIL® holey carbon (2/1) copper grids (Ref. Q225CR-06; Quantifoil Micro Tools GmbH, Großlöbichau, Germany) after glow discharge with argon plasma. Next, grids were blotted to remove the liquid excess and vitrified in ethane in a Leica Automatic Plunge Freezer EM GP2 (Leica, Wetzlar, Germany). Samples were maintained in liquid nitrogen and detected using a JEM-2200FS/CR transmission electron microscope (JEOL Ltd., Akishima, Japan).
Jem 2200fs cr transmission electron microscope
Manufactured by JEOL
Sourced in Japan
The JEM-2200FS/CR is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of materials at the nanoscale. The microscope uses an electron beam to interact with the sample, allowing users to observe and study the detailed structure and composition of a wide range of materials.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using jem 2200fs cr transmission electron microscope
1
Cryo-EM analysis of extracellular vesicles
2
Vitrification and Imaging of sEVs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
sEVs were directly adsorbed onto glow-discharged holey carbon grids (QUANTIFOIL, Großlöbichau, Germany). Grids were then blotted at 95% humidity and rapidly plunged into liquid ethane with the aid of a VITROBOT (Maastricht Instruments BV, Maastricht, The Netherlands). Vitrified samples were imaged at liquid nitrogen temperature using a JEM-2200FS/CR Transmission Electron Microscope (JEOL, Tokyo, Japan), equipped with a field emission gun, and operated at an acceleration voltage of 200 kV.
3
Transmission Electron Microscopy Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron micrographs were taken on a JEOL 2100 microscope with thermionic gun LaB6 200 kV equipped with Gatan Orius high resolution CCD camera. TEM samples were prepared over Formvar/Carbon film on 200 mesh copper grids. Gels: Fresh gels were applied directly onto a grid and expelled solvent was carefully removed by capillarity with paper. The grids were immediately stained with one drop of 1% aqueous phosphotungstic acid for 2 min and the liquid was subsequently removed by capillary action. Nanogels: A drop of nanogels suspension was added over a grid and incubated for 2 min. Then, solvent was removed with filter paper by capillarity and a drop of OsO4 0.1% was added. After 5 min, the staining solution was removed by capillarity and the grid was washed with a drop of miliQ water. For Cryo-TEM technique, JEM-2200FS/CR transmission electron microscope (JEOL, Japan), equipped with an UltraScan 4000 SP (4008x4008 pixels) cooled slow-scan CCD camera (GATAN, UK) was used. A drop of the nanogel suspension was placed on the TEM grid and an automated vitrification robot Vitrobot TM was used to freeze the sample in liquid ethane.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!