Confirm mouse anti er antibody clone 6f11

Before 1988, ER and PgR were determined using isoelectric focusing on polyacrylamide gel as previously described [5 (link)]. After 1988, an enzyme immunoassay was used. For ER, studies have shown that results with these techniques correlate well with those obtained using methods based on dextran-coated charcoal and IHC [2 (link)]. The cytosol receptor values were normalized to DNA content, and a receptor content of ≥0.05 fmol/µg DNA was classified as positive. The IHC staining was performed using the Ventana HX automatic system BenchMark (Ventana Medical System, SA IllKirch, Cedex, France). Primary monoclonal antibodies were the CONFIRM™ mouse anti-ER antibody (clone 6F11) and the CONFIRM™ mouse anti-PR antibody (clone 16) from Ventana Medical Systems. Antigen retrieval and staining procedure were performed according to the instruction by the Ventana manufacture. Positive controls were run with each batch. Only the invasive part of the carcinoma was assessed, and for each case, all three cores of the TMA were reviewed. The receptor levels presented are based on an average of the three cores of the TMA. The proportion of stained nuclei was recorded as 0, 1–9 %, 10–24 %, 25–49 %, 50–74 %, 75–89 %, and ≥90 %. The scoring was done by two pathologists (B.L; L.S.).

ER and PR were detected by immunohistochemistry using CONFIRM™ mouse anti-ER antibody (clone 6F11) and CONFIRM™ mouse anti-PR antibody (clone 16) from Ventana Medical Systems. The staining was performed according to the manufacturer instructions using the Ventana^® automated slide stainer (Ventana Medical Systems, S.A., Illkirch, France). HER2 was detected with the DAKO AO0485 polyclonal rabbit antibody also according to the guidelines provided by the manufacturer [35 (link)].
Ki67 was stained with the monoclonal mouse anti-human Ki67 clone MIB-1 (DAKO M7240) using the DAKO Link48 Auto Stainer protocol [36 (link)].