We used a 15% SDS-PAGE gel to isolate proteins, and the proteins were transferred to a PVDF membrane. The primary antibodies used in our study were mouse anti-GAPDH (1:5000; Santa Cruz, USA), rabbit anti-RPL35 (1:100; ABclonal, China), rabbit anti-DDX10 (1:300; Proteintech, USA), mouse anti-FLAG (1:2,000; Sigma, USA), rabbit anti-RPL18 (1:100; ABclonal, China), rabbit anti-HNRNPU (1:100; ABclonal, China), rabbit anti-NCL (1:100; ABclonal, China), rabbit anti-PRMT5 (1:100; ABclonal, China), rabbit anti-E2F1 (1:100; ABclonal, China), rabbit anti-E2F4 (1:100; ABclonal, China), rabbit anti-E2F8 (1:100; ABclonal, China), goat anti-rabbit IgG (1:2,000; CST, USA), and goat anti-mouse IgG (1:2,000; CST, USA).
Rabbit anti ddx10
Manufactured by Proteintech
Sourced in China, United Kingdom
Rabbit anti-DDX10 is a primary antibody that targets the DDX10 protein. DDX10 is a member of the DEAD-box RNA helicase family, which are involved in various cellular processes such as transcription, translation, and RNA processing. This antibody can be used for the detection of DDX10 in various experimental applications.
Automatically generated - may contain errors
2 protocols using rabbit anti ddx10
1
Western Blot Characterization of Cellular Proteins
2
Western Blot Protein Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We used a 15% SDS-PAGE gel to isolate proteins, and the proteins were transferred to a PVDF membrane. The primary antibodies used in our study were mouse anti-GAPDH (1:5,000; Santa Cruz, USA), rabbit anti-RPL35 (1:300; ABCAM, UK), rabbit anti-DDX10 (1:300; Proteintech, USA), mouse anti-FLAG (1:2,000; Sigma, USA), rabbit anti-RPL18 (1:1,000; ABCAM, UK), rabbit anti-SRFBP1 (1:2,000; ABCAM, UK), rabbit anti-HNRNPU (1:2,000; Proteintech, USA), rabbit anti-nucleolin (1:1,000; ABCAM, UK), rabbit anti-PRMT5 (1:10,000; ABCAM, UK), goat anti-rabbit IgG (1:2,000; CST, USA), and goat anti-mouse IgG (1:2,000; CST, USA).
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