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β cadherin

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β‐cadherin is a cell adhesion protein that plays a role in cell-cell adhesion and signaling processes. It is a member of the cadherin family of calcium-dependent cell-cell adhesion molecules.

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Lab products found in correlation

Vimentin, by Cell Signaling Technology (2 mentions) E cadherin, by Cell Signaling Technology (2 mentions) Phosphor pi3k, by Cell Signaling Technology (1 mentions) Phosphor mtor, by Cell Signaling Technology (1 mentions) Phosphor akt, by Arigo Biolaboratories (1 mentions) Gapdh, by Arigo Biolaboratories (1 mentions) Bca protein assay kit, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions) Human recombinant tgfβ, by R&D Systems (1 mentions) Primary antibody against β actin, by Merck Group (1 mentions) Goat anti rabbit igg, by Merck Group (1 mentions) Emodin, by Merck Group (1 mentions) Hrp conjugated goat anti mouse igg, by Merck Group (1 mentions) Mmp 2, by Cell Signaling Technology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) P creb, by Cell Signaling Technology (1 mentions) P src, by Cell Signaling Technology (1 mentions) Prsk2, by Cell Signaling Technology (1 mentions) Anti phospho perk1 2, by Cell Signaling Technology (1 mentions)

3 protocols using β cadherin

1

Protein Expression Analysis in Cell Lysates

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Cell lysis was prepared on ice and subjected to centrifugation at 4°C, 14 000 g/min for 10 minutes. Proteins were quantified using a BCA Protein Assay Kit (Sigma‐Aldrich) and separated by 10% SDS‐PAGE gel. Subsequently, proteins were loaded on a PVDF membrane (Millipore, Billerica, MA), and incubated with specific antibodies. Antibodies were purchased from Abcam (SELM, PI3K, phosphor‐PI3K, mTOR, phosphor‐mTOR, vimentin, N‐cadherin, and β‐cadherin), Cell Signaling Technology (Akt, phosphor‐Akt, MMP2, MMP9, anti‐rabbit, and anti‐mouse secondary antibodies), and Arigo (GAPDH).
Jiang H., Shi Q., Ge L., Zhuang Q., Xue D., Xu H, & He X. (2019). Selenoprotein M stimulates the proliferative and metastatic capacities of renal cell carcinoma through activating the PI3K/AKT/mTOR pathway. Cancer Medicine, 8(10), 4836-4844.
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2

Epithelial-Mesenchymal Transition Protein Analysis

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The compounds emodin and 4’,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Human recombinant TGF-β was purchased from R&D Systems (Minneapolis, MN, USA). Primary antibodies against Snail, E-cadherin, β-cadherin, MMP-2 and Slug were purchased from Cell Signaling Technology (Beverly, MA, USA). Primary antibody against vimentin was purchased from Abcam Inc. (Cambridge, MA, USA). Primary antibodies against Twist were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Primary antibody against β-actin was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Secondary antibodies, HRP-conjugated Goat anti-Mouse IgG and Goat anti-Rabbit IgG, were obtained from Millipore (Billerica, MA, USA).
Hsu H.C., Liu L.C., Wang H.Y., Hung C.M., Lin Y.C., Ho C.T, & Way T.D. (2017). Stromal Fibroblasts from the Interface Zone of Triple Negative Breast Carcinomas Induced Epithelial-Mesenchymal Transition and its Inhibition by Emodin. PLoS ONE, 12(1), e0164661.
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3

Immunoblot Analysis of Signaling Pathways in Tumor Xenografts

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Protein concentrations of the total cellular protein extracts from tumor xenografts and cell line lysates were determined using the Bio-Rad protein assay (Bio-Rad, Hercules, CA, USA). Total protein (50 μg per sample) from either tumor xenograft or cell line lysates was electrophorectically separated on 10% SDS polyacrylamide gels and electro-blotted onto a Hybond membrane. After incubation with 5% nonfat milk in Tris-buffered saline containing 0.1% Tween-20, the blots were probed with anti-phospho p-Erk1/2 (Thr202/Tyr204), p-Creb (Ser133), p-Rsk2 (Ser386), p-Src (Tyr416), p-Fak (Tyr576/577), p-Pax (y118), p-Pkcα (s657), and p-Stat3 (y705), polyclonal rabbit antibodies (Cell Signaling Technology Inc., Davners, MA). The same blots were subsequently stripped and re-probed with anti-total (t) Her2, Her3, Erk1/2, cSrc, Creb, Fax, Pax, Pkcα, Rsk2, Stat3, Fascin, E-Cadherin, β-Cadherin, Vimentin, Snail, and β-Actin antibodies (Cell Signaling Technology Inc.).
Mao L., Summers W., Xiang S., Yuan L., Dauchy R.T., Reynolds A., Wren-Dail M.A., Pointer D., Frasch T., Blask D.E, & Hill S.M. (2016). Melatonin Represses Metastasis in Her2-postive Human Breast Cancer Cells by Suppressing RSK2 Expression. Molecular cancer research : MCR, 14(11), 1159-1169.
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