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Mouse anti rabbit igg antibody alkaline phosphatase

Manufactured by Merck Group
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The Mouse anti-rabbit IgG antibody:alkaline phosphatase is a laboratory reagent used in various immunoassay techniques. It consists of a mouse-derived antibody that specifically binds to rabbit immunoglobulin G (IgG) molecules, conjugated with the enzyme alkaline phosphatase. This conjugate can be used to detect and quantify the presence of rabbit IgG in biological samples.

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Lab products found in correlation

Sigmafast bcip nbt, by LI COR (2 mentions) Signalfire elite ecl reagent, by LI COR (2 mentions) C digit chemiluminescent blot scanner, by LI COR (2 mentions) Monoclonal murine anti polyhistidine antibody, by Merck Group (2 mentions) Phospho threonine antibody, by Cell Signaling Technology (2 mentions) Anti mouse igg whole molecule alkaline phosphatase antibody, by Merck Group (2 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (2 mentions) Trans blot turbo transfer system, by Bio-Rad (2 mentions)

Close spelling variants of this product

Anti-rabbit IgG-alkaline phosphatase antibody Anti-mouse IgG-Alkaline Phosphatase antibody Anti-mouse IgG-alkaline phosphatase Alkaline phosphatase Rabbit anti-IgG Anti-IgG mouse IgG rabbit IgG mouse Phosphatases Rabbit anti-

2 protocols using mouse anti rabbit igg antibody alkaline phosphatase

1

Immunoblotting of Mycobacterial Proteins

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Proteins were separated on 4–20% gradient SERVA gels and transferred onto a nitrocellulose membrane using a Trans‐Blot® Turbo™ Transfer System (Bio‐Rad). SIGMAFAST™ BCIP®/NBT or SignalFire™ Elite ECL Reagent were used to visualise proteins on C‐DiGit Chemiluminescent Blot Scanner (LI‐COR Biosciences). The following antibodies were used: custom polyclonal antibody raised against Lsr2 in rabbit (Gemini Biosciences); monoclonal murine anti‐polyhistidine antibody (Sigma‐Aldrich); phospho‐threonine antibody (Cell Signaling Technology); monoclonal anti‐MtbGroEL2 (Rv0440), clone IT‐70 (BEIResources); mouse anti‐rabbit IgG antibody:alkaline phosphatase (Sigma‐Aldrich; anti‐mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma‐Aldrich), and anti‐rabbit IgG, HRP‐linked antibody (Cell Signaling Technology).
Alqaseer K., Turapov O., Barthe P., Jagatia H., De Visch A., Roumestand C., Wegrzyn M., Bartek I.L., Voskuil M.I., O'Hare H.M., Ajuh P., Bottrill A.R., Witney A.A., Cohen‐Gonsaud M., Waddell S.J, & Mukamolova G.V. (2019). Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112. Molecular Microbiology, 112(6), 1847-1862.
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2

Western Blot Analysis of Lsr2 Protein

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Proteins were separated on 4–20% gradient SERVA gels and transferred onto a nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (Bio-Rad). SIGMAFAST™ BCIP®/NBT or SignalFire™ Elite ECL Reagent were used to visualise proteins on C-DiGit Chemiluminescent Blot Scanner (LI-COR Biosciences). The following antibodies were used: custom polyclonal antibody raised against Lsr2 in rabbit (Gemini Biosciences); monoclonal murine anti-polyhistidine antibody (Sigma-Aldrich); phospho-threonine antibody (Cell Signaling Technology); monoclonal anti-MtbGroEL2 (Rv0440), clone IT-70 (BEIResources); mouse anti-rabbit IgG antibody:alkaline phosphatase (Sigma-Aldrich; anti-mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma-Aldrich), and anti-rabbit IgG, HRP-linked antibody (Cell Signaling Technology).
Alqaseer K., Turapov O., Barthe P., Jagatia H., De Visch A., Roumestand C., Wegrzyn M., Bartek I.L., Voskuil M.I., O'Hare H.M., Ajuh P., Bottrill A.R., Witney A.A., Cohen‐Gonsaud M., Waddell S.J, & Mukamolova G.V. (2019). Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112. Molecular Microbiology, 112(6), 1847-1862.
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