We performed immunofluorescence staining by a published procedure [27 (link)]. Lung sections were treated with primary rabbit polyclonal antibody, claudin-18 (diluted 1:200, Proteintech, IL, USA), and phosphorylated-SPAK (p-SPAK) (diluted 1:100, OriGene, MD, USA) for immunofluorescent labeling. The secondary antibody was goat anti-mouse IgG-FITC (diluted 1:200, Santa Cruz Biotechnology, USA) and Rhodamine (TRITC) AffiniPure Goat Anti-Rabbit IgG (diluted 1:200, Jackson ImmunoResearch Inc. PA, USA). The slides were mounted with VECTASHIELD Antifade Mounting Medium (Vector Laboratories, Inc. CA, USA) and DAPI. Images were obtained using a DeltaVision system (Applied Precision) comprising a wide-field inverted microscope (model IX-71; Olympus) with × 60/1.42 Plan Apo N or × 100/1.40 Super-Plan APO objectives.
Phosphorylated spak p spak
Manufactured by OriGene
Sourced in United States
Phosphorylated-SPAK (p-SPAK) is a recombinant protein produced by OriGene. It represents the phosphorylated form of the SPAK (STE20/SPS1-related proline-alanine-rich kinase) protein. SPAK is a serine/threonine-protein kinase that plays a role in the regulation of ion homeostasis and cell volume.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti mouse igg fitc, by Santa Cruz Biotechnology (2 mentions)
Claudin 18, by Proteintech (2 mentions)
Deltavision system, by Cytiva (2 mentions)
Vectashield antifade mounting medium, by Vector Laboratories (2 mentions)
Rhodamine tritc affinipure goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
2 protocols using phosphorylated spak p spak
1
Immunofluorescent Labeling of Lung Claudin-18 and p-SPAK
2
Immunofluorescent Localization of Claudin-18 and p-SPAK
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunofluorescence staining was performed using a published procedure [28] . We treated lung sections with primary rabbit polyclonal antibody, claudin-18 (diluted 1:200, Proteintech, IL, USA), and phosphorylated-SPAK (p-SPAK) (diluted 1:100, OriGene, MD, USA) for immunofluorescent labeling. The secondary antibody was goat anti-mouse IgG-FITC (diluted 1:200, Santa Cruz Biotechnology, USA) and Rhodamine (TRITC) A niPure Goat Anti-Rabbit IgG (diluted 1:200, Jackson ImmunoResearch Inc. PA, USA). The slides were mounted with VECTASHIELD Antifade Mounting Medium (Vector Laboratories, Inc. CA, USA) and DAPI. Images were obtained using a DeltaVision system (Applied Precision) comprising a wide-field inverted microscope (model IX-71; Olympus) with ×60/1.42 Plan Apo N or ×100/1.40 Super-Plan APO objectives.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!