Du897 emccd

Manufactured by Oxford Instruments

Sourced in Japan

The DU897 EMCCD is a high-performance electron-multiplying charge-coupled device (EMCCD) camera. It features a large sensor size, high quantum efficiency, and low readout noise, making it suitable for various low-light imaging applications.

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Lab products found in correlation

Nis elements 5, by Nikon (1 mentions) Perfect focus system, by Nikon (1 mentions) Ti2 e, by Nikon (1 mentions) N storm microscope, by Nikon (1 mentions) Elements ar software, by Nikon (1 mentions) Csu x1 spinning disk head, by Oxford Instruments (1 mentions) Eclipse ti e inverted, by Yokogawa (1 mentions) Nis elements software, by Nikon (1 mentions) Ti e inverted microscope, by Oxford Instruments (1 mentions)

Close spelling variants of this product

DU897 (90 citations) DU897 EMCCD camera (39 citations) IXon X3 DU897 EMCCD camera (2 citations) DU897 Ultra EMCCD camera (2 citations) IXON3 Ultra DU897 EMCCD (2 citations)

10 protocols using du897 emccd

In Vivo Imaging of mPEG-C/S/S-MUCNPs

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Firstly, 50 μL of mPEG-C/S/S-MUCNPs (500 μg/mL) were injected intradermally into the subcutaneous tissue of nude mice. The system includes two external adjustable CW 980 nm lasers (0–5 W) (Shanghai Connet Fiber Optics Co., Shanghai, China) sources and an Andor DU897 EMCCD as the signal collector. Images of luminescent signals were processed with the Kodak Molecular Imaging software (Rochester, NY, USA).

Luo Y., Zhang W., Liao Z., Yang S., Yang S., Li X., Zuo F, & Luo J. (2018). Role of Mn2+ Doping in the Preparation of Core-Shell Structured Fe3O4@upconversion Nanoparticles and Their Applications in T1/T2-Weighted Magnetic Resonance Imaging, Upconversion Luminescent Imaging and Near-Infrared Activated Photodynamic Therapy. Nanomaterials, 8(7), 466.

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Confocal Imaging of Cellular Dynamics

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All confocal images presented in this study were captured using a Nikon A1Rsi point scanning confocal microscopy with a Nikon Ti2‐E motorized inverted microscopy and Perfect Focus System. Images were taken with an Andor DU897 EMCCD and a 100× objective immersed with oil (CFI Plan ApochromatVC N.A. = 1.40). The system was driven by NIS Elements 5.01 (Nikon) software. Time‐lapse imaging for cell tracking and proliferation analysis was recorded using an IMQ Biostation with a 37 °C, 5% CO2 humidified chamber. The imaging interval was 5 mins for 96 h and images were taken using a 10× objective.

Jiang K., Lim S.B., Xiao J., Jokhun D.S., Shang M., Song X., Zhang P., Liang L., Low B.C., Shivashankar G.V, & Lim C.T. (2023). Deleterious Mechanical Deformation Selects Mechanoresilient Cancer Cells with Enhanced Proliferation and Chemoresistance. Advanced Science, 10(22), 2201663.

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Quantum Dot Imaging Protocol

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The reaction products were diluted 100-fold in the imaging buffer (1 mg mL^–1 glucose oxidase, 0.4% (w/v) d-glucose, 0.04% mg mL^–1 catalase, 50 μg mL^–1 BSA, 67 mM glycine–KOH, 1 mg mL^–1 trolox, 2.5 mM MgCl₂, pH 9.4). For TIRF imaging, 10 μL of samples was directly pipetted to the coverslips. A sapphire 488 nm laser (50 mW, Coherent, U.S.A.) was used to excite the 605QDs. The photons from the 605QD and Cy5 were collected by a 100× objective (Olympus, Japan) and imaged with an exposure time of 100 ms by an Andor Ixon DU897 EMCCD. Generally, six frames of images from six different locations were acquired for every sample and a region of interest (200 × 400 pixels) of each image was selected for Cy5 molecule counting through the image J software.

Wang Z.Y., Wang L.J., Zhang Q., Tang B, & Zhang C.Y. (2017). Single quantum dot-based nanosensor for sensitive detection of 5-methylcytosine at both CpG and non-CpG sites †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc04813k. Chemical Science, 9(5), 1330-1338.

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Automated Microscopy for Immobilized Samples

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Animals were immobilized with 0.4mM levamisole and mounted on 10% agarose pads in M9 buffer. Volumes over time were captured using an automated TiE inverted fluorescence microscope platform with an X1 spinning disk head (Yokogawa) and DU-897 EM-CCD (Andor Technology), piezo-electric Z stage (Mad City Labs) and Apo TIRF 100x 1.49 NA oil immersion objective (Nikon Instruments, Inc.). Analysis of fluorescence intensity was accomplished using NIS-Elements and Fiji/Image J software.

Sundararajan L., Smith C.J., Watson J.D., Millis B.A., Tyska M.J, & Miller DM I.I.I. (2019). Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response. PLoS Genetics, 15(6), e1008228.

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Single-Molecule TIRF Imaging of QDs

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For TIRF imaging, 10 μL of samples was directly pipetted onto the coverslips. A sapphire 488 nm laser (50 mW, Coherent, USA) was used to excite the QDs. The photons from the QD and Cy5 were collected by a 100× objective (Olympus, Japan) and imaged with an exposure time of 100 ms by an Andor Ixon DU897 EMCCD. A region of interest (600 × 600 pixels) of the images was selected for Cy5 molecule counting by using image J software.

Wang Z.Y., Li D.L., Tian X, & Zhang C.Y. (2021). A copper-free and enzyme-free click chemistry-mediated single quantum dot nanosensor for accurate detection of microRNAs in cancer cells and tissues. Chemical Science, 12(31), 10426-10435.

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Visualizing FtsZ Dynamics in TIRF Microscopy

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FtsZ dynamics were imaged by near-TIRF illumination on a Nikon N-STORM microscope equipped with an NA 1.49 Nikon 100× TIRF objective, laser source (488 nm), additional 2.5× magnification optics and a 16 μm pixel Andor iXon DU897 EMCCD, giving a final pixel size at the image plane of 64 nm. Exposure times were 1 s and images were taken at 1 s intervals. The temperature at the sample was 30.0°C.

Bisson Filho A.W., Hsu Y.P., Squyres G.R., Kuru E., Wu F., Jukes C., Sun Y., Dekker C., Holden S., VanNieuwenhze M.S., Brun Y.V, & Garner E.C. (2017). Treadmilling by FtsZ Filaments Drives Peptidoglycan Synthesis and Bacterial Cell Division. Science (New York, N.Y.), 355(6326), 739-743.

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Live Imaging and Cortical Ablation

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Live imaging and cortical ablation were performed on a Nikon Eclipse Ti-E inverted microscope equipped with a Yokogawa CSU-X1 spinning disk head, 1.4 NA 60X oil objective, Andor DU-897 EMCCD and a dedicated 100 mW 405 diode ablation laser, generously provided by the Nikon Centre of Excellence at Vanderbilt University. The instrument was controlled using Nikon Elements AR software. For ablation, a 1.4 μm x 1.4 μm ROI was used for all experiments. A DIC and/or fluorescence image was acquired before ablation, followed by ablation using a miniscanner. A pixel dwell time of 500 ms, 50% laser power was used for a duration of 1 s, followed by acquiring DIC or fluorescence images at 2 s intervals. Samples were maintained at 37°C with 5% CO₂ using Tokai Hit Stage Incubator.
To image MIIA and MIIB in fixed ESC colonies, large image stitching was performed using the 60X objective in Elements software. Z sections were acquired at 1 mm intervals for the entire stitch and maximum projections were displayed and used for generating line scans. To image endogenous MIIA and MIIB in fixed HeLa cells, single slices through the middle of the cell were acquired using the 60X objective.

Taneja N., Bersi M.R., Baillargeon S.M., Fenix A.M., Cooper J.A., Ohi R., Gama V., Merryman W.D, & Burnette D.T. (2020). Precise Tuning of Cortical Contractility Regulates Cell Shape during Cytokinesis. Cell reports, 31(1), 107477.

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Axin2 Expression Analysis by RNAscope

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RNAScope technology (ACD Bio) was used to perform RNA in situ hybridization (RNA ISH) experiments (Wang et al., 2012 (link)) according to manufacturer’s instructions, with the Axin2 (400331) probe used. IF images were acquired using an automated TiE inverted fluorescence microscope platform equipped with an encoded motorized stage and Plan Apo 60× 1.40 NA objective (Nikon Instruments, Inc.). The Nikon TiE inverted fluorescence confocal microscope was outfitted with a Yokogawa X1 spinning disk head and Andor DU-897 EM-CCD. Lasers utilized for excitation included 405, 488, 561, and 647nm, and emission filters were 455/50, 525/36, 641/75, and 700/74 (peak/bandwidth), respectively. NIS-Elements software (Nikon Instruments, Inc.) was utilized for image acquisition.

Aros C.J., Vijayaraj P., Pantoja C.J., Bisht B., Meneses L.K., Sandlin J.M., Tse J.A., Chen M.W., Purkayastha A., Shia D.W., Sucre J.M., Rickabaugh T.M., Vladar E.K., Paul M.K, & Gomperts B.N. (2020). Distinct spatiotemporally dynamic Wnt-secreting niches regulate proximal airway regeneration and aging. Cell stem cell, 27(3), 413-429.e4.

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Quantifying Lipid Membrane Composition

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The abundance of cholesterol (Chol), SM, PC, and PE on the cell membrane was analyzed by a commercial TIRFM (Nikon TiE Inverted Microscope) equipped with a 100 × 1.49 NA objective and an Andor iXon DU897 EMCCD. During imaging, BODIPY-cholesterol and NBD-SM were excited by a 488-nm laser, and the emission was collected by using a 500–550 nm band-pass filter. TMR-PC and TMR-PE were excited by a 561-nm laser, and the emission was collected by using a 580–620 nm band-pass filter. After the respective process, we imaged the fluorescence intensity of the bottom surface of each group of cells and then used the ImageJ program to perform statistical analysis (about 30 cells per group).

Ruan H., Zou C., Xu Y., Fang X., Xia T, & Shi Y. (2022). N-(3-Oxododecanoyl) Homoserine Lactone Is a Generalizable Plasma Membrane Lipid-Ordered Domain Modifier. Frontiers in Physiology, 12, 758458.

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Hybrid Color-Fluorescence Imaging System

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A home-made EagleRay-V3 imaging system previously developed for intraoperative imaging 38 (link) was employed for the experimental measurements herein. The EagleRay-V3 system is a hybrid color-fluorescence system employing an iXon-3 electron multiplying charge-coupled device (DU-897 EMCCD; Andor Technology, Belfast, Ireland) for fluorescence detection and a 12-bit-CCD camera (pixelfly qe, PCO, Kelheim, Germany) for color detection. A 300-mW continuous laser diode (BWF2-750-0, B&W Tek, Newark) at 750 nm was used for fluorescence excitation and a 250-W halogen lamp (KL-2500 LCD, Scott, Mainz, Germany) was employed for white-light illumination.

Anastasopoulou M., Koch M., Gorpas D., Karlas A., Klemm U., Garcia-Allende P.B, & Ntziachristos V. (2016). Comprehensive phantom for interventional fluorescence molecular imaging. Journal of biomedical optics, 21(9).

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