HPLC-grade solvents, ascorbic acid, potassium hydroxide (KOH) and butylated hydroxytoluene were obtained from Fisher (Fair Lawn, NJ, USA). dl-Tocol (internal standard, purity >95%) was purchased from Matreya LLC (Pleasant Gap, PA, USA) and α-tocopheryl acetate was purchased from Sigma (St. Louis, MO, USA). NGT is not available commercially and was synthesized from γ-T as described [7 (link)]. Briefly, an ethanolic solution of γ-T was acidified with glacial acetic acid and nitration was subsequently induced by sodium nitrite. After terminating the reaction with KOH, and washing the mixture with water, the crude product was extracted using hexane, dried under nitrogen gas and reconstituted in ethanol. Purity of NGT was >99% as determined by LC-MS and its concentration was determined spectrophotometrically (Shimadzu UV-2600, Columbia, MD, USA) in ethanol using ε410 nm = 1976 M−1cm−1 [12 (link)].
α tocopheryl acetate
Manufactured by Merck Group
Sourced in United States
α-tocopheryl acetate is a form of vitamin E commonly used in laboratory applications. It serves as a chemical standard and reference material for analytical procedures involving vitamin E. The compound provides a stable and well-defined source of the α-tocopherol form of vitamin E for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Butylated hydroxy toluene, by Thermo Fisher Scientific (1 mentions)
Ascorbic acid, by Thermo Fisher Scientific (1 mentions)
Potassium hydroxide koh, by Thermo Fisher Scientific (1 mentions)
Uv 2600, by Shimadzu (1 mentions)
Absolute ethanol, by Thermo Fisher Scientific (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Retinyl acetate, by Merck Group (1 mentions)
Dl α tocopherol, by Merck Group (1 mentions)
Anhydrous sodium sulfate, by Honeywell (1 mentions)
Direct q uv, by Merck Group (1 mentions)
Lc ms grade methanol, by Merck Group (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
α tocopherol, by Merck Group (1 mentions)
Retinol, by Merck Group (1 mentions)
3 protocols using α tocopheryl acetate
1
Synthesis and Characterization of NGT
2
Simultaneous Quantification of Fat-Soluble Vitamins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the standards (retinol, retinyl acetate, dl-α-tocopherol, α-toco-pheryl acetate and cholecalciferol), the isotope- labeled internal standards of cholecalciferol (6,19,19-d3 solution) and α-tocopherol (phenyl-5,7-dimethyl-d6)) as well as L-ascorbic acid, butylated hydroxyl-toluene (BHT), α-amylase from Aspergillus oryzae and sodium hydroxide were purchased from Sigma Aldrich (Steinheim, Germany). Methanol, ethanol, and Acetonitrile HPLC grade, and absolute ethanol and petroleum ether analytical grade, were obtained from Fischer Scientific (Geel, Belgium). Acetonitrile and Methanol LC-MS grade were acquired from Merck (Darmstadt, Germany), while anhydrous sodium sulfate was purchased from Honeywell (Offenbach, Germany). Distilled water was provided by a MilliQ purification apparatus (Millipore Direct-Q UV, Bedford, MA, USA). Chromafil Regenerated Cellulose (RC) syringe filters (15 mm diameter, 0.2 mm pore size) were obtained from Macherey-Nagel (Düren, Germany). Solid-phase extraction (SPE) Strata Silica 500 mg/6 mL cartridges (55 m, 70Å) were purchased from Phenomenex (Torrance, CA, USA).
3
Vitamin and Mineral Standard Procurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We purchased standard Cu, Fe, Mn, Zn, Ca, and Mg from Buck Scientific, USA, and the immunoglobulin kits from Chronolab, Switzerland. Sigma Chemical Co., USA, supplied the standard α-tocopherol (vitamin E), α-tocopheryl acetate, and retinol (vitamin A). For HPLC-based analysis, Active Fine Chemicals Limited, Dhaka, Bangladesh, supplied HPLC-grade chemicals and reagents. We purchased other supportive chemicals of the recommended grade from Merck, Germany.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!