Anti collagen 1 and 3

Manufactured by Abcam

Sourced in United Kingdom

Anti-Collagen I and III is a laboratory reagent used to detect and/or quantify Collagen I and III in various samples. It is a specific antibody that binds to these collagen types.

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Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (2 mentions) Anti β actin, by Merck Group (1 mentions) Anti cd45, by Abcam (1 mentions) Rabbit anti ace2, by Abcam (1 mentions) Anti il 6, by Abcam (1 mentions) Anti cd68, by Abcam (1 mentions)

Spelling variants of this product

Collagen I (278 citations) Anti-collagen I (151 citations) Collagen III (84 citations) Anti-collagen III (31 citations) Collagen I and collagen III (3 citations) Anti-collagen I and – collagen III (2 citations)

2 protocols using anti collagen 1 and 3

Quantitative Collagen Analysis

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Cell extracts were performed by using RIPA buffer (Thermo Fisher Scientific). The samples were analyzed by western blotting using the following antibodies: anti-Collagen I and III (Abcam), anti-β actin (Millipore).

Sivasubramanian S., Chandrasekar G., Svensson Akusjärvi S., Thangam R., Sathuvan M., Kumar R.B., Hussein H., Vincent S., Madhan B., Gunasekaran P, & Kitambi S.S. (2017). Phenotypic Screening Identifies Synergistically Acting Natural Product Enhancing the Performance of Biomaterial Based Wound Healing. Frontiers in Pharmacology, 8, 433.

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Histopathological Analysis of Mouse Hearts

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Mouse hearts were dissected and fixed in 4% formalin. Heart sections were embedded in paraffin and sectioned to 5-μm thickness for staining with hematoxylin and eosin (H&E). Extracellular matrix (ECM) deposition was valued by staining with masson's trichrome. Slides were incubated overnight with the following primary antibodies: rabbit anti-ACE2, anti-collagen I and III, anti-CD45, anti-CD68 and anti-IL-6 (all Abcam, Cambridge，UK). The sections were incubated with a goat antibody (ZSGB-BIO, Peking, China) as the secondary antibody. Matrix accumulation was quantified using Image-Pro Plus 6.0.

Zhai C.G., Xu Y.Y., Tie Y.Y., Zhang Y., Chen W.Q., Ji X.P., Mao Y., Qiao L., Cheng J., Xu Q.B, & Zhang C. (2018). DKK3 overexpression attenuates cardiac hypertrophy and fibrosis in an angiotensin-perfused animal model by regulating the ADAM17/ACE2 and GSK-3β/β-catenin pathways. Journal of molecular and cellular cardiology, 114.

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