Ps176 180 ikkα β

Manufactured by Cell Signaling Technology

Sourced in United States

PS176/180-IKKα/β is a rabbit monoclonal antibody that recognizes IKKα/β when phosphorylated at serines 176 and 180. This antibody can be used to detect the activation of the IKKα/β complex, which is a key step in the NF-κB signaling pathway.

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Lab products found in correlation

Pt183 y185 jnk, by Cell Signaling Technology (2 mentions) Pt202 y204 erk, by Cell Signaling Technology (2 mentions) Ps32 36 iκbα, by Cell Signaling Technology (2 mentions) Py416 c src, by Cell Signaling Technology (2 mentions) Pt180 y182 p38, by Promega (2 mentions) Mouse trip6, by BD (2 mentions) Traf6, by Santa Cruz Biotechnology (2 mentions) C src, by Santa Cruz Biotechnology (2 mentions) Histone h3, by Cell Signaling Technology (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Gapdh, by Santa Cruz Biotechnology (2 mentions) Caspase 8, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg h l hrp, by Dianova (1 mentions) Gapdh, by Merck Group (1 mentions)

3 protocols using ps176 180 ikkα β

Immunoblotting, Co-IP, and Recombinant Protein Purification

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Experimental procedures of immunoblotting, co-immunoprecipitation, and purification of recombinant glutathione-S-transferase (GST)-tagged or non-tagged proteins were performed as described previously [19 (link), 34 (link)]. Immunoblotting was performed using antibody specific to human TRIP6, A20 (Bethyl Laboratories), pT183/Y185-JNK, pT202/Y204-ERK, pS32/36-IκBα, pS176/180-IKKα/β, pY416-c-Src, CYLD, IκBα, IKKβ, Histone H3 (Cell Signaling, Danvers, MA, USA), pT180/Y182-p38 (Promega, Madison, WI, USA), ERK, JNK, mouse TRIP6 (BD Biosciences, San Jose, CA, USA), HA (Cayman, Ann Arbor, MI, USA), FLAG (Sigma-Aldrich), GFP, TRAF6, p38, c-Src, GAPDH, GST, ubiquitin, or phosphotyrosine (Santa Cruz Biotechnology, Dallas, TX, USA).

Lin F.T., Lin V.Y., Lin V.T, & Lin W.C. (2016). TRIP6 antagonizes the recruitment of A20 and CYLD to TRAF6 to promote the LPA2 receptor-mediated TRAF6 activation. Cell Discovery, 2, 15048-.

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Immunoblotting and Protein Purification Protocols

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Experimental procedures of immunoblotting, co-immunoprecipitation, and purification of recombinant glutathione-S-transferase (GST)-tagged or non-tagged proteins were performed as described previously [19 (link), 34 (link)]. Immunoblotting was performed using antibody specific to human TRIP6, A20 (Bethyl Laboratories), pT183/Y185-JNK, pT202/Y204-ERK, pS32/36-IκBα, pS176/180-IKKα/β, pY416-c-Src, CYLD, IκBα, IKKβ, Histone H3 (Cell Signaling, Danvers, MA, USA), pT180/Y182-p38 (Promega, Madison, WI, USA), ERK, JNK, mouse TRIP6 (BD Biosciences, San Jose, CA, USA), HA (Cayman, Ann Arbor, MI, USA), FLAG (Sigma-Aldrich), GFP, TRAF6, p38, c-Src, GAPDH, GST, ubiquitin, or phospho-tyrosine (Santa Cruz Biotechnology, Dallas, TX, USA).

Lin F.T., Lin V.Y., Lin V.T, & Lin W.C. (2016). TRIP6 antagonizes the recruitment of A20 and CYLD to TRAF6 to promote the LPA2 receptor-mediated TRAF6 activation. Cell Discovery, 2, 15048-.

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Comprehensive Signaling Pathway Analysis

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MLKL (#14993), Caspase-8 (#4927), p38 MAPK (#9212), MAPKAPK2 (D1E11) (#12155), pS166-RIPK1 (#31122), pS345-MLKL (#37333), phospho-p44/42 Erk1/2) (Thr202/Tyr204) (pERK1/2) (#4370), phospho-p38 (Thr180/Tyr182) (pp38) (#9215), phospho-SAPK/JNK (Thr183/Tyr185) (pJNK) (G9) (#9255), SAPK/JNK (#9252), pS32/36-IkBα (#9246), IkBα (#9242), pS176/180-IKKα/β (#2697), IKKα (#2682) antibodies were from Cell Signaling Technology (CST). Further antibodies used were against RIPK1 (#610459, BD Biosciences), GAPDH (#MAB374, Millipore), EF2 (sc-166415, Santa Cruz Biotechnology), ERK2 (sc-154, Santa Cruz Biotechnology). The secondary antibodies used were goat anti-mouse IgG (H+L)-HRP (#115-035-003, Dianova) and goat anti-rabbit IgG (H+L)-HRP (111-035-003, Dianova).

Chauhan C., Kraemer A., Knapp S., Windheim M., Kotlyarov A., Menon M.B, & Gaestel M. (2023). 5-Iodotubercidin sensitizes cells to RIPK1-dependent necroptosis by interfering with NFκB signaling. Cell Death Discovery, 9, 262.

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