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Anti p73

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Anti-p73 is a protein that binds and detects the presence of the p73 protein. It is commonly used in laboratory research applications.

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Lab products found in correlation

Anti chk1, by Cell Signaling Technology (2 mentions) Anti brca1, by Cell Signaling Technology (2 mentions) Anti e2f1, by Cell Signaling Technology (2 mentions) Anti p chk1, by Cell Signaling Technology (2 mentions) Anti gapdh, by Santa Cruz Biotechnology (2 mentions) Anti chk2, by Cell Signaling Technology (2 mentions) Anti p53, by Merck Group (2 mentions) Anti atr, by Cell Signaling Technology (2 mentions) Anti nbs1, by Cell Signaling Technology (2 mentions) Anti mre11, by Cell Signaling Technology (2 mentions) Anti p chk2, by Cell Signaling Technology (2 mentions) Anti topbp1, by Santa Cruz Biotechnology (2 mentions) Anti p63, by Cell Signaling Technology (2 mentions) Anti brca2, by Cell Signaling Technology (2 mentions) Anti rad51, by Cell Signaling Technology (2 mentions) Anti keratin 10, by Santa Cruz Biotechnology (2 mentions) Anti loricrin, by Santa Cruz Biotechnology (2 mentions) Anti p topbp1, by Abcepta (2 mentions) Anti p atr, by Cell Signaling Technology (2 mentions) Ecl prime, by Cytiva (2 mentions)

6 protocols using anti p73

1

Antibody Characterization for EMT Research

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The following antibodies were used: rabbit polyclonal anti-Slug (1:1000), anti-Twist1 (1:500), anti-ZEB1 and anti-ZEB2 (1:500, Santa Cruz Biotechnology, Santa Cruz, CA). Rabbit monoclonal anti-E-cadherin (1:1000, Santa Cruz). Rabbit polyclonal anti-MDM2 (C-18) (1:500, Santa Cruz). Mouse monoclonal anti-α-tubulin (1:2000) and anti-fibronectin (1:1000) (Cell Signaling, Danvers, MA). Mouse monoclonal anti-N-cadherin (1:500), anti-ZO-1 (1:1000) and anti-vimentin (1:1000) (Abcam, Cambridge, UK). Mouse monoclonal anti-Restin (1:500), anti-His (1:1500) and anti-Flag (1:2000) (Sigma). Mouse monoclonal anti-p73 (1:1000), anti-p53 (1:1000) and anti-p63 (1:1000) (Abcam). Horseradish peroxidase–conjugated secondary antibodies were obtained from Amersham Biosciences.
Lu Z., Jiao D., Qiao J., Yang S., Yan M., Cui S, & Liu Z. (2015). Restin suppressed epithelial-mesenchymal transition and tumor metastasis in breast cancer cells through upregulating mir-200a/b expression via association with p73. Molecular Cancer, 14, 102.
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2

Antibody Panel for Cellular Signaling

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The following antibodies were used in this study: anti-PGAM1 (NBP1-49532; Novus Biologicals), anti-CtIP (sc-271339; Santa Cruz Biotechnology, Inc.), anti-Mre11 (ab214; Abcam), anti–H2AX-pS139 (9718; Cell Signaling Technology), anti–β-actin (60008-1-Ig; Proteintech), anti–Lamin B1 (12987-1-AP; Proteintech), anti-GAPDH (60004-1-Ig; Proteintech), anti-RPA32 (ab2175; Abcam), anti–RPA32-pS4S8 (NBP1-23017; Novus Biologicals), anti-BrdU (5292; Cell Signaling Technology), anti-Cdh1 (ab3242; Abcam), anti-p21 (2947; Cell Signaling Technology), anti-RAD51 (sc-8349; Santa Cruz Biotechnology, Inc.), anti-PGD (sc-398977; Santa Cruz Biotechnology, Inc.), anti-PHGDH (ab57030; Abcam), anti-IgG (2729; Cell Signaling Technology), anti-Histone H3 (4620; Cell Signaling Technology), anti-p53 (9282; Cell Signaling Technology), anti-p73 (ab202474; Abcam), and anti–Cleaved Caspase-3 (9661; Cell Signaling Technology).
Qu J., Sun W., Zhong J., Lv H., Zhu M., Xu J., Jin N., Xie Z., Tan M., Lin S.H., Geng M., Ding J, & Huang M. (2017). Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells. The Journal of Cell Biology, 216(2), 409-424.
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3

Western Blot Protein Analysis

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Proteins were separated by electrophoresis based on its molecular weight and transferred from the gel to a PVDF membrane. Then, primary antibodies probing the target protein after the PVDF membrane were blocked by bovine serum albumin. The first antibodies were then detected by second antibodies, which could recognize them conjugated to enzyme horseradish peroxidase. The information of antibodies were as follows: anti-Rb (Epitomics, Burlingame, USA), anti-E2F1(Epitomics, Burlingame, USA), anti-P/CAF monoclonal antibodies (Santa Cruz Biotechnology, Dallas, USA), anti-p73 (Abcam, Boston, USA) and anti-Caspase7 (Abcam, Boston, USA).
Ma W., Yu J., Qi X., Liang L., Zhang Y., Ding Y., Lin X., Li G, & Ding Y. (2015). Radiation-induced microrna-622 causes radioresistance in colorectal cancer cells by down-regulating Rb. Oncotarget, 6(18), 15984-15994.
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4

Western Blot Analysis of DNA Damage Response Proteins

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The antibodies from this study were as follows: anti-keratin-10, anti-loricrin, anti-TopBP1, and anti-GAPDH (Santa Cruz, Santa Cruz, CA); anti-E2F1, anti-p63, anti-ATR, anti-p-ATR, anti-CHK1, anti-p-CHK1, anti-CHK2, anti-p-CHK2, anti-RAD51, anti-Mre11, anti-NBS1, anti-BRCA1, and anti-BRCA2 (Cell Signaling, Danvers, MA); anti-p73 (Abcam, Cambridge, MA); anti-p53 (Millipore, Burlington, MA); anti-p-TopBP1 (Abgent, San Diego, CA). AKT inhibitors MK2206 (2μM) and LY294002 (25μM) were purchased from SelleckChem (Houston, TX). Cell lysates were processed and assayed by western blot analysis as previously described 60 (link). Briefly, J2 feeders were firstly removed by Versene (PBS containing 0.5 mM EDTA) treatment and keratinocytes were collected and lysed in RIPA lysis buffer on ice for 30 minutes. The samples were then electrophoresed on SDS-page gels and transferred to PVDF membranes. At last the membranes were developed using ECL prime or ECL reagents (Amersham, Pittsburgh, PA) and chemiluminescence signals were detected using Eastman Kodak x-ray films.
Hong S., Xu J., Li Y., Andrade J., Hoover P., Kaminski P.J, & Laimins L.A. (2019). Topoisomerase IIβ-binding protein 1 activates expression of E2F1 and p73 in HPV positive cells for genome amplification upon epithelial differentiation. Oncogene, 38(17), 3274-3287.
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5

Western Blot Analysis of Apoptosis-Related Proteins

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Total cell lysates were generated using lysis buffer (50 mmol/l Tris-HCl pH 7.6, 250 mmol/l NaCl, 0.1% Triton X-100, 5 mmol/l EDTA) supplemented with complete protease inhibitor cocktail (Roche, Grenzach-Wyhlen, Germany) and PhosSTOP Phosphatase Inhibitor Cocktail (Roche). After sonification and boiling, a specific amount of protein was resuspended in Laemmli and analyzed by western blotting. The primary antibodies used were as follows: anti-p53 (clone: DO-1—#sc-126, Pab1801—#sc-98), anti-p63 (clone: 4A4—#sc-8431) (Santa-Cruz Biotechnology, Heidelberg, Germany); anti-BAX (polyclonal—#2772), anti-BAK (polyclonal—#3814), anti-JNK1 (clone: 2C6—#3708) (Cell Signaling, Denvers, MA, USA); anti-p73 (polyclonal—#ab14430) (Abcam, Cambridge, UK); anti-phospho-c-Jun (Ser73) (polyclonal—#06-659) (Upstate, Charlottesville, VA, USA).
Weilbacher A., Gutekunst M., Oren M., Aulitzky W.E, & van der Kuip H. (2014). RITA can induce cell death in p53-defective cells independently of p53 function via activation of JNK/SAPK and p38. Cell Death & Disease, 5(7), e1318-.
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6

Western Blot Analysis of DNA Damage Response Proteins

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The antibodies from this study were as follows: anti-keratin-10, anti-loricrin, anti-TopBP1, and anti-GAPDH (Santa Cruz, Santa Cruz, CA); anti-E2F1, anti-p63, anti-ATR, anti-p-ATR, anti-CHK1, anti-p-CHK1, anti-CHK2, anti-p-CHK2, anti-RAD51, anti-Mre11, anti-NBS1, anti-BRCA1, and anti-BRCA2 (Cell Signaling, Danvers, MA); anti-p73 (Abcam, Cambridge, MA); anti-p53 (Millipore, Burlington, MA); anti-p-TopBP1 (Abgent, San Diego, CA). AKT inhibitors MK2206 (2μM) and LY294002 (25μM) were purchased from SelleckChem (Houston, TX). Cell lysates were processed and assayed by western blot analysis as previously described 60 (link). Briefly, J2 feeders were firstly removed by Versene (PBS containing 0.5 mM EDTA) treatment and keratinocytes were collected and lysed in RIPA lysis buffer on ice for 30 minutes. The samples were then electrophoresed on SDS-page gels and transferred to PVDF membranes. At last the membranes were developed using ECL prime or ECL reagents (Amersham, Pittsburgh, PA) and chemiluminescence signals were detected using Eastman Kodak x-ray films.
Hong S., Xu J., Li Y., Andrade J., Hoover P., Kaminski P.J, & Laimins L.A. (2019). Topoisomerase IIβ-binding protein 1 activates expression of E2F1 and p73 in HPV positive cells for genome amplification upon epithelial differentiation. Oncogene, 38(17), 3274-3287.
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