Wild-type male C57BL/6J mice, IL-6KO mice, and RAG-/- mice were purchased from The Jackson Laboratory (Bar Harbor, ME). All gene-deficient mice used are on the C57BL/6 background. IFNAR-/-48 were provided by Dr. Paul W. Dempsey (Department of Microbiology and Molecular Genetics, University of California, Los Angeles) and Dr. Tadatsugu Taniguchi (Department of Immunology, Tokyo University, Japan) to Dr. W. Overwijk and crossed to the C56BL/6 background. IL15Rα-/- mice49 were originally generated by and obtained from Dr. Averil Ma through Dr. Leo Lefrancois and crossed to the C57BL/6 background. All animal experiments were performed according to the institutional guidelines for the care and use of laboratory animals.
Wild type c57bl 6j male mice
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
Wild-type C57BL/6J male mice are a commonly used inbred mouse strain. They serve as a standard laboratory animal model.
Automatically generated - may contain errors
Lab products found in correlation
B6 5 lepob j, by Jackson ImmunoResearch (2 mentions)
Extract n amp tissue pcr kit, by Merck Group (2 mentions)
Pomc cre male mice, by Jackson ImmunoResearch (2 mentions)
Rag mice, by Jackson ImmunoResearch (1 mentions)
Il 6 ko mice, by Jackson ImmunoResearch (1 mentions)
Cg tg k18 ace2 2prlmn j, by Jackson ImmunoResearch (1 mentions)
K18 hace2 c57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
Ai9 cre reporter mice, by Jackson ImmunoResearch (1 mentions)
R26 hm4di mcitrine, by Jackson ImmunoResearch (1 mentions)
Alzet 1002, by Durect (1 mentions)
Standard laboratory chow, by Inotiv (1 mentions)
Avertin, by Merck Group (1 mentions)
C57bl 6j female mice, by Jackson ImmunoResearch (1 mentions)
Male cd 1 retired breeder mice, by Charles River Laboratories (1 mentions)
Orca flash4.0 v2 scmos, by Hamamatsu Photonics (1 mentions)
C57bl 6 ins2akita j mice, by Jackson ImmunoResearch (1 mentions)
Male new zealand white rabbits, by Charles River Laboratories (1 mentions)
50 protocols using wild type c57bl 6j male mice
1
Mouse Strains for Immunology Research
2
C57BL/6J Mice Animal Study Protocol
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Wild-type male C57BL/6J mice were purchased from Jackson Labs. All procedures involving animals were undertaken in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals by the National Institutes of Health. The study protocol was approved by the University Committee on the Use and Care of Animals at the University of Michigan (PRO00008999 and PRO00010712).
3
Murine and Human Cell Lines
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Wild-type male C57BL/6J mice were purchased from The Jackson laboratory (Bar Harbour, ME, USA). NT2196 and NMuMG cells were kindly provided by Dr. William Muller (McGill University, Montréal, Canada) and have been described elsewhere [30 (link)]. MCF7 and MCF10A cells were purchased from ATCC. All reagents were purchased from Sigma-Aldrich unless otherwise stated.
4
C57BL/6J Mice Experimental Protocol
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Wild-type male C57BL/6J mice (8–12 weeks of age) were obtained from Jackson Laboratories (Bar Harbor, Maine) and housed in pathogen-free conditions for 1 week prior to experiments. This study was approved by the Institutional Animal Care and Use Committee and the Animal Care and Use Review Office of the US Army and conformed to the ARRIVE guidelines. The ARRIVE checklist is included in the Supplemental Digital Content (http://links.lww.com/TA/D54 ).
5
C57BL/6J Mouse Housing Protocols
6
SARS-CoV-2 Mouse Model Protocols
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All mice studies were conducted in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. Animal experiments were performed as specified in protocols approved by the Institutional Animal Care and Use Committee (IACUC) at the Washington University School of Medicine (Assurance Number: A3381–01). All dissections and virus inoculations were performed under anesthesia, induced and maintained by using ketamine hydrochloride and xylazine, and every effort was made to minimize suffering. Wild-type male C57BL/6J mice (cat no. 000664) were obtained from The Jackson Laboratory and used at 6 to 8 weeks of age for all experiments. Heterozygous K18-hACE2 C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J, cat no. 34860) were obtained from The Jackson Laboratory. Stat6tm1Gru (Stat6−/−) (005977) were purchased from Jackson Laboratories and bred at Washington University under specific pathogen-free conditions. 10BiT reporter mice crossed to Foxp3gfp reporter mice were kindly provided by Chyi Song Hsieh (Washington University in St. Louis)42 (link). All mice were housed in the animal facility, fed standard chow diets, and kept at ~70°F with humidity at ~50%, and the dark/light cycle was 12 h/12 h.
7
Bafiliomycin A1 Treatment in C57BL/6J Mice
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Wild type male C57BL/6J mice (#000664) at 10 weeks of age were purchased from Jackson Laboratories (Bar Harbor, ME, USA). Mice were randomized to receive either BafA1 or vehicle.
8
Remote Ischemic Postconditioning Neuroprotection
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The Institutional Animal Care and Use Committee of Georgia Regents University (GRU) approved the experimental procedures as per the National Institute of Health guidelines. C57BL/6 J wild-type male mice (10 ± 1 weeks old) were procured from Jackson Laboratory (Bar Harbor, Maine) and were housed in GRU’s AAALAC accredited facility. In these experiments, we use the term “remote ischemic postconditioning” (RIPostC) as we start the conditioning 1 week after the VCI procedure for 2-week period. We randomized 20 mice into 3 groups: a sham-operated group for procedures of BCAS and therapy (Sham group, N = 5), an untreated BCAS/VCI-induced group (with RIPostC sham procedure), i.e., BCAS group (N = 7), and a VCI-induced RIPostC-treated BCAS + RPostC-group (N = 8). The outcome measures were blinded. Cognitive function and CBF changes were considered as the primary outcomes to calculate the sample size of 5 in each group to provide >80 % power at α = 0.05, as reported by us earlier [10 (link)].
9
Behavioral Experiments on Hcrt Neurons
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All behavioural experiments conducted to target Hcrt neurons were done on C57BL/6 J wild-type male mice obtained at ~7 weeks of age from the Jackson Laboratory. LepRb::IRES-cre knockin mice69 (link) were kindly provided by Dr Martin G. Jr Myers (University of Michigan, Ann Arbor, MI, USA) and bred (>6 generations) onto a C57BL6/J background. LepRb::cre transgenic mice homozygous for the obese spontaneous mutation LepOb/Ob were obtained by intercrossing heterozygous LepOb/+ mice (B6.V-Lepob/J from the Jackson Laboratory, stock number 000632) with LepRb::cre mice. Mice were housed in individual Plexiglas recording chambers in custom-designed stainless-steel cabinets at constant temperature (22±1 °C), humidity (40–60%) and circadian cycle (12-h light–dark cycle, starting at 0900 hours). Food and water were available ad libitum except for chronic FR procedures (see Stress procedures). All experiments were performed in accordance with the guidelines described in the US National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of Stanford University.
10
C57BL/6J Male Mice Experiments
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C57BL/6J, wild-type male mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA). The animal use protocol TAMU #2019-0144 was approved by the Texas A&M University Institutional Animal Care and Use Committee and procedures were performed in accordance with the NIH Guidelines for the Care and Use of Laboratory Animals. All mice were between the ages of 10–17 weeks of age and weighed ~25–30 g each.
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