Alpha tubulin antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Alpha-tubulin antibody is a laboratory research tool used to detect and study alpha-tubulin, a protein component of the cytoskeleton in eukaryotic cells. It can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to visualize and analyze the distribution and expression of alpha-tubulin in biological samples.

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Lab products found in correlation

Pjnk antibodies, by Cell Signaling Technology (1 mentions) Cyp2e1 antibody, by Merck Group (1 mentions) Acetaminophen apap, by Merck Group (1 mentions) Tri reagent, by Thermo Fisher Scientific (1 mentions) Rnase free dnase, by Promega (1 mentions) Ecl chemiluminescence detection kit, by Thermo Fisher Scientific (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Rip3 antibody, by ProSci (1 mentions) Growth supplement, by Lonza (1 mentions) Fibroblast basal medium, by Lonza (1 mentions) Nhlfs, by Lonza (1 mentions) Alexa fluor 488 conjugated donkey anti rabbit secondary antibody, by Abcam (1 mentions) Sb431542, by R&D Systems (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Acetylated α tubulin 6 11b 1, by Santa Cruz Biotechnology (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) Bortezomib, by Selleck Chemicals (1 mentions) Mm 1r, by American Type Culture Collection (1 mentions) Vimentin antibody, by Abcam (1 mentions) General tissue culture materials, by Avantor (1 mentions)

Close spelling variants of this product

Alpha-tubulin (26 citations) Anti-alpha-tubulin antibody (3 citations) Anti-alpha-tubulin mouse monoclonal antibody (2 citations)

6 protocols using alpha tubulin antibody

Cytosolic and Nuclear Protein Extraction

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Cells were washed with ice‐cold PBS twice and harvested in PBS. One‐fourth of the cells were transferred to a new Eppendorf. PBS was removed after centrifuging the cells at 1,500 g for 3 min. The total lysate was collected by adding a RIPA lysis buffer (10 mM Tris, pH 7; 150 mM NaCl; 1% Triton X‐100; 1% Na deoxycholate; 1% SDS; 1× protease inhibitor) into one‐fourth of the cells. The remaining cells were lysed by an NP‐40 lysis buffer (1× TBS; 0.5% NP‐40; 1× protease inhibitor). The suspension, containing cytosolic proteins, was transferred into a new Eppendorf. The remaining pellets were washed with an NP‐40 lysis buffer twice and then lysed by a RIPA lysis buffer as a nuclear fraction. Alpha‐tubulin antibody (Santa Cruz Biotechnology) was used as a cytosolic marker, while antibody against Lamin B2 (Millipore) was used as a nuclear marker. All experiments were repeated three times. Histograms were the means ± SD from the three independent experiments.

Wang Y., Huang J., Chen W., Wang R., Kao M., Pan Y., Chan S., Tsai K., Kung H., Lin K, & Wang L. (2019). Dysregulation of cystathionine γ‐lyase promotes prostate cancer progression and metastasis. EMBO Reports, 20(10), e45986.

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Acetaminophen Toxicity Mechanism Analysis

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Acetaminophen (APAP) was purchased from Sigma Chemical Co. (St. Louis, MO). RIP1, JNK and pJNK antibodies were from Cell Signaling. RIP3 antibody was from ProSci Incorporated (San Diego, California, USA). Alpha-tubulin antibody was from Santa Cruz Biotechnologies (Santa Cruz, CA, USA). CYP2E1 antibody was from Milipore (Temecula, California, USA). Chemiluminescence (ECL) detection kit was from Pierce Biotechnology (Rockford, IL, USA). TRI reagent was from Invitrogen (Carlsbad, CA, USA). RNase-free DNase was from Promega Corporation (Madison, WI, USA). In Situ Cell Death Detection Kit (Roche, Cat. No. 11684817910). All other reagents were purchased from Sigma Chemical Co. (St. Louis, MO) if not otherwise stated.

Lu Y., Zhang C., Chen Y.H., Wang H., Zhang Z.H., Chen X, & Xu D.X. (2017). Immature mice are more susceptible than adult mice to acetaminophen-induced acute liver injury. Scientific Reports, 7, 42736.

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Fibroblast Characterization and Activation

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Fibroblast basal medium, growth supplements, and NHLFs were purchased from Lonza (Walkersville, MD). Human-derived transforming growth factor beta (TGFβ), as well as the TGFβ receptor blocker SB431542, was obtained from R&D Systems, Inc. (Minneapolis, MN). Poly-L-lysine was purchased from Sigma-Aldrich (St. Louis, MO). The anti-Collagen I antibody was purchased from Fitzgerald (Acton, MA), while the alpha tubulin antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). Alexa-fluor 488-conjugated donkey anti-rabbit secondary antibody, alexa-fluor 488-conjugated alpha-smooth muscle actin (α-SMA) primary antibody, and the beta actin primary antibody were purchased from Abcam (Cambridge, MA). ProLong gold antifade with DAPI mountant was obtained from Molecular Probes (Thermo Fisher Scientific, Waltham, MA).

Davidson D.C., Derk R., He X., Stueckle T.A., Cohen J., Pirela S.V., Demokritou P., Rojanasakul Y, & Wang L. (2016). Direct stimulation of human fibroblasts by nCeO2in vitro is attenuated with an amorphous silica coating. Particle and Fibre Toxicology, 13, 23.

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Evaluating Bortezomib and SAHA Effects on Myeloma

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Dexamethasone (Dex)-sensitive MM.1S and Dex-resistant MM.1R were obtained from the American Type Culture Collection (Manassas, VA, USA). RPMI-8226 and U266, NCI-H929, SKO, and LP1 human MM cells were provided by Changzheng Hospital, Shanghai. All MM cell lines were cultured with RPMI-1640 + 10% FBS medium. BIS was synthesized in Shanghai Institute of Materia Medica. BIS was dissolved first in DMSO at a concentration of 10 mM, and then in culture medium (0.5–8 μM) immediately before use. SAHA was purchased from MedExpress company.
Bortezomib was obtained from Selleck Chemicals for the in vitro studies. It was dissolved first in DMSO at a concentration of 20 mM, and then in culture medium before use. Bortezomib formulated with mannitol was dissolved in saline for the in vivo studies.
Histone H3, acetyl-Histone H3 (Lys23), and poly(ADP-ribose) polymerase antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Acetylated-α-tubulin (6-11B-1) and alpha-tubulin antibody were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Zhou Y.B., Zhang Y.M., Huang H.H., Shen L.J., Han X.F., Hu X.B., Yu S.D., Gao A.H., Sheng L., Su M.B., Wei X.L., Zhang Y., Zhang Y.F., Gao Z.W., Chen X.Y., Nan F.J., Li J, & Hou J. (2021). Pharmacodynamic, pharmacokinetic, and phase 1a study of bisthianostat, a novel histone deacetylase inhibitor, for the treatment of relapsed or refractory multiple myeloma. Acta Pharmacologica Sinica, 43(4), 1091-1099.

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Chromatin Immunoprecipitation and Transcription Factor Regulation

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General tissue culture materials were obtained from VWR International. Antibodies against Snail and BRD4 were obtained from Abcam. Antibodies against c-MYC, p21 and FOSL1 were purchased from Cell Signaling, HMGA2 antibody was from Biocheck Inc, while vimentin antibody was from Abcam. Alpha-tubulin antibody was obtained from Santa Cruz, while E-cadherin antibody was from BD Bioscience. Secondary antibodies were purchased from Sigma. The EZ-Chip and EZ-Zyme Chromatin Prep kits were from Millipore. The anti-BRD4 antibody for ChIP assay was purchased from Bethyl Laboratories, while the anti-RNA polymerase II antibody and control IgG antibody were from Millipore. BET inhibitor JQ1 was obtained from BPS Bioscience, while I-BET151 was acquired from Tocris Bioscience. BRD4, c-MYC, and FOSL1 siRNAs were purchased from Life Technologies.

Sahai V., Kumar K., Knab L.M., Chow C.R., Raza S.S., Bentrem D.J., Ebine K, & Munshi H.G. (2014). BET bromodomain inhibitors block growth of pancreatic cancer cells in three-dimensional collagen. Molecular cancer therapeutics, 13(7), 1907-1917.

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Western Blot Analysis of Phospho-Erk

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Cells were seeded in 6-well plates at a density of 1×10⁵ cells per well. After 24h, cells were starved in starvation medium (DMEM or RPMI media supplemented with 1% penicillin/streptomycin/glutamine, and 20 mM HEPES). Cells were lysed in ice-cold RIPA buffer supplemented with protease (cOmplete, Sigma #4693159001) and phosphatase inhibitors (PhosSTOP, Sigma #4906845001). After a 10 min centrifugation at 4°C, supernatants were supplemented with 5X Laemmli’s sample buffer and were boiled for 10 min. SDS PAGE was performed in with in NuPAGE Bis-Tris gels (Invitrogen) using MES buffer (ThermoFisher #NP0002), and blots were transferred onto nitrocellulose membranes using the BioRad Trans-Blot Semi-Dry Transfer Cell. Transferred blots were then blocked with Odyssey blocking buffer (LI-COR #927–4000) and antibody stained using the Freedom Rocker liquid handling system. Western blots were imaged on a LI-COR Odyssey imager, and images were quantified using ImageJ. Phospho-Erk antibody was obtained from Cell Signaling Technologies (#4370), alpha- tubulin antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, #23948,1:1000), and IRDye conjugated secondary antibodies (#926–3221, #926–68020) were obtained from LI-COR.

Bugaj L.J., Sabnis A.J., Mitchell A., Garbarino J.E., Toettcher J.E., Bivona T.G, & Lim W.A. (2018). Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway. Science (New York, N.Y.), 361(6405), eaao3048.

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