The RAW 264.7 murine macrophage cell line was obtained from the Korea Cell Line Bank (Seoul, Korea) and maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% (v/v) fetal bovine serum, L-glutamine (2 mM), penicillin (100 U/ml) and streptomycin (100 U/ml) (all from WelGENE Inc., Daegu, Korea) at 37°C in a humidified atmosphere containing 5% CO2 and 95% air. Schisandrin A was purchased from Sigma-Aldrich (cat. no. SML0054; Merck KGaA, Darmstadt, Germany), dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich; Merck KGaA) and final concentrations were adjusted by dilution with complete culture medium. The DMSO concentration did not exceed 0.05% (i.e., a non-cytotoxic range). To stimulate the cells, the medium was replaced with fresh DMEM, and LPS (E. coli Serotype 055:B5; cat. no. L2880; Sigma-Aldrich; Merck KGaA) was added in the presence or absence of Schisandrin A for the indicated periods.
Schisandrin a
Manufactured by Merck Group
Sourced in Sao Tome and Principe, United States
Schisandrin A is a pure compound isolated from the fruit of the Schisandra chinensis plant. It is a white crystalline solid with a molecular formula of C24H32O7. Schisandrin A is commonly used as a reference standard in analytical testing and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Streptomycin, by Welgene (1 mentions)
L glutamine, by Welgene (1 mentions)
Raw 264.7 murine macrophage cell line, by Korean Cell Line Bank (1 mentions)
Penicillin, by Welgene (1 mentions)
P iκbα, by Cell Signaling Technology (1 mentions)
Recombinant rat il 1β 501 rl 010, by R&D Systems (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Pge2 elisa kit, by R&D Systems (1 mentions)
Collagenase 2, by Merck Group (1 mentions)
P jnk, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by GenDEPOT (1 mentions)
Dulbecco s modified eagle s medium dmem, by GenDEPOT (1 mentions)
Schisandrol a, by Merck Group (1 mentions)
Trypsin ethylenediaminetetraacetic acid edta, by Thermo Fisher Scientific (1 mentions)
Hydrogen peroxide h2o2, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt dimethyl sulfoxide dmso, by Merck Group (1 mentions)
3 protocols using schisandrin a
1
Schisandrin A Modulates Macrophages
2
Schisandrin A Modulates Inflammatory Responses
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Schisandrin A, dimethylsulfoxide (DMSO) and collagenase II were obtained from Sigma-Aldrich (St. Louis, MO, United States). Schisandrin A was dissolved in DMSO and stored at −80°C. Control group was added with DMSO (Vehicle) in the cell experiments. Recombinant rat IL-1β (501-RL-010) and PGE2 ELISA kit were procured from R&D Systems (Minneapolis, MN, United States). Dulbecco’s modified Eagle’s medium F12 (DMEM/F12) was purchased from HyClone (Grand Island, NY, United States). Antibodies against MMP1, p65, Collagen II, and IκBα were provided from Proteintech Group (Wuhan, China). Antibodies specific for MMP13 and iNOS were purchased from Abcam (Cambridge, United Kingdom). Antibodies against COX2, MMP3, p-p65, p-IκBα, ERK1/2, p-ERK, p38, p-p38, JNK, p-JNK were supplied by Cell Signaling Technology (Beverly, MA, United States). Antibodies against Aggrecan was acquired from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Antibodies specific for GAPDH, ADAMTS5 and secondary antibodies were procured from Boster (Wuhan, China).
3
Antioxidant Activity Evaluation
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Schisandrol A, schisandrol B, schisandrin A, gomisin N, schisandrin C, 2,2-diphenyl-2-picrylhydrazyl (DPPH), L-ascorbic acid, potassium persulfate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), and hydrogen peroxide (H2O2) were procured from Sigma-Aldrich (St. Louis, MO, USA). The 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) single solution was obtained from Invitrogen (Camarillo, CA, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were purchased from GenDEPOT (Barker, TX, USA). Penicillin-streptomycin and trypsin-ethylenediaminetetraacetic acid (EDTA) were obtained from Gibco (Grand Island, NY, USA). TRI-Solution™ was purchased from BioScience Technology (Daegu, Korea).
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